Clarification of xenobiotic recognition mechanism at the blood-placentabarrier by transporter array

2007 Fiscal Year Final Research Report Summary

• Project/Area Number: 18590154
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Medical pharmacy
• Research Institution: Kyoritsu University of Pharmacy
• Principal Investigator: SAI Yoshimichi Kyoritsu University of Pharmacy, Department of Phairmaceutics, Associate Professor (40262589)
• Co-Investigator(Kenkyū-buntansha): NAKASHIMA Emi Kyoritsu University of Pharmacy, Department of Pharmaceutics, Professor (90115254) ; KOSE Noriko Kyoritsu University of Pharmacy, Department of Pharmaceutics, Research Fellow (60348612)
• Project Period (FY): 2006 – 2007
• Keywords: Placenta barrier / Nucleoside / Nucleobase / Transporter / Drug Drug Interaction / Contribution / Anti-virus drug / recognition of xenobiotics

Research Abstract

The uptake mechanisms of nucleosides at the blood-placenta barrier were clarified by using the rat syncytiotrophoblast cell line TR-TBT 18d-1. The initial uptake of [3H]uridine and [3H]adenosine by TR-TBT 18d-1 were sodium-independent and were sensitive to nitrobenzylthioinosine. The Km value of adenosine uptake was approximately 17 μM. These results suggested involvement of equilibrative nucleoside transporters ENT1 and ENT2. The uptakes were significantly reduced by several nucleoside drugs, including zidovudine (AZT), cytarabine, vidarabine, mizoribine and caffeine. The effects were small within the therapeutic concentration ranges. Therefore, these drugs at the therapeutic concentrations might have little influence on maternal-to-fetal nucleoside transfer. The uptake mechanism of AZT was characterized. Initial uptake of AZT was sodium-independent and saturable (Km, approx. 16 μM). Thymidine and 2' -deoxyuridine strongly inhibited the uptake, but nitrobenzylthioinosine, probenecid a nd cimetidine had little effect on the uptake. Cyclosporin A, Ko143 and probenecid had little effect on the steady state AZT accumulation, suggesting that transporter-mediated efflux of AZT is not substantial. These results indicate that the saturable AZT uptake by TR-TBT 18d-1 was mediated by a so-far unidentified transporter.
A human enterocyte model Caco-2 cell exhibits estrone-3-sulfate uptake, but the responsible transporters have not been clarified, yet. We have examined the contribution of OATP2B1 to the uptake in comparison with that of its homolog genes. The OATP2B1 mRNA expression was higher than that of OATP3A1 or OATP4A1 in Caco-2 and in human jejunum biopsies. The estrone-3-sulfate uptakes normalized to OATP2B1 mRNA expression were similar in Caco-2 cells and HEK293/OATP2B1 cells. The specific activity of OATP2B1 per mRNA expression was much higher than that of OATP3A1 and OATP4A1. These results suggest that OATP2B1 is predominantly responsible for the uptake in Caco-2 cells. This method can be applied to determine the contribution of each transporter in the placenta barrier.

Research Products

• [Journal Article] Characterization of the mechanism of zidovudine uptake by rat conditionally immortalized syncytiotrophoblast cell line TR-TBT (2008)
  • Author(s): Sai Y, Nishimura T, Shimpo S, Chishu T, Sato K, Kose N, TerasakiT, Mukai C, Kitagaki S, Miyakoshi N, Kang YS, Nakashima E.
  • Journal Title: Pharm Res (in press)
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Potential of various drugs to inhibit nucleoside uptake in rat syncytiotrophoblast cell line,TR-TBT 18d-1 (2008)
  • Author(s): Chishu T, Sai Y, Nishimura T, Sato K, Kose N, Nakashima E.
  • Journal Title: Placenta 29 Pages: 461-467
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Involvement of multidrug resistance-associated protein 2 (Abcc2) in molecular weight-dependent biliary excretion of {beta}浜actam antibiotics (2008)
  • Author(s): Kato Y, Takahara S, Kato S, Kubo Y, Sai Y, Tamai I, Yabuuchi H, Tsuji A.
  • Journal Title: Drug Metab Dispos 36 Pages: 1088-1096
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] New streams of membrane molecule research toward drug development and therapy (2008)
  • Author(s): Ohtsuki S, Sai Y
  • Journal Title: Yakugaku Zasshi 128 Pages: 495-496
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Characterization of the mechanism of zidovudine uptake by rat conditionally immortalized syncytiotrophoblast cell line TR-TBT (2008)
  • Author(s): Sai, Y, Nishimura, T, Shimpo, S, Chishu, T, Sato, K, Kose, N, Terasaki, T, Mukai, C, Kitagaki, S, Miyakoshi, N, Kang, YS, Nakashima, E
  • Journal Title: Pharm Res (in press)
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Potential of various drugs to inhibit nucleoside uptake in rat syncytiotrophoblast cell line, TR-TBT 18d-1 (2008)
  • Author(s): Chishu, T, Sai, Y, Nishimura, T, Sato, K, Kose, N, Nakashima, E
  • Journal Title: Placenta 29 Pages: 461-467
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Involvement of multidrug resistance-associated protein 2 (Abcc2) in molecular weight-dependent biliary excretion of {beta}-lactam antibiotics (2008)
  • Author(s): Kato, Y, Takahara, S, Kato, S, Kubo, Y, Sai, Y, Tamai, I, Yabuuchi, H, Tsuji, A
  • Journal Title: Drug Metab Dispos 36 Pages: 1088-1096
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Transport of carnitine and acetylcarnitine by carnitine/organic cation transporter (OCTN) 2 and OCTN3 into epididymal spermatozoa (2007)
  • Author(s): Kobayasbi D, Tamai I, Sai Y, Yoshida K, Wakayama T, Kido Y, Nezu J, Iseki S, Tsuji A.
  • Journal Title: Reproduction 134 Pages: 651-658
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Heterophilic binding of the adhesion molecules poliovirus receptor and immunoglobulin superfamily 4A in the interaction between mouse spermatogenic and Sertoli cells (2007)
  • Author(s): Wakayama T, Sai Y, Ito A, Kato Y, Murakami Y, Nakashima E, Tsuji A, Kitamura Y, Iseki S.
  • Journal Title: Biol Reprod 76 Pages: 1081-1090
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Altered expression of basement membrane-related molecules in rat brain pericyte,endothelial,and astrocyte cell lines after transforming growth factor-β1 treatment (2007)
  • Author(s): Kose N, Asashima T, Muta M, Iizasa H, Sai Y, Terasaki T, Nakashima E.
  • Journal Title: Drug Metab Pharmacokinet 22 Pages: 255-266
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Hepatocyte growth factor and c-Met expression in pericytes implications for atherosclerotic plaque development (2007)
  • Author(s): Liu Y, Wilkinson F, Kirton J, Jeziorska M, Iizasa H, Sai Y, Nakashima E, Heagerty A, Canfield A, Alexander M.
  • Journal Title: J Pathol 212 Pages: 12-19
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Characterization of the uptake mechanism for a novel loop diuretic,M17055,in Caco-2 cells;involvement of organic anion transporting poly peptide (OATP)-B (2007)
  • Author(s): Nishimura T, Kubo Y, Kato Y, Sai Y, Ogihara T, Tsuji A.
  • Journal Title: Pharm Res 24 Pages: 90-99
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Transport of carnitine and acetylcarnitine by carnitine/organic cation transporter (OCTN) 2 and OCTN3 into epididymal spermatozoa (2007)
  • Author(s): Kobayashi, D, Tamai, I, Sai, Y, Yoshida, K, Wakayama, T, Kido, Y, Nezu, J, Iseki, S, Tsuji, A
  • Journal Title: Reproduction 134 Pages: 651-658
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Heterophilic binding of the adhesion molecules poliovirus receptor and immunoglobulin superfamily 4A in the interaction between mouse spermatogenic and Sertoli cells (2007)
  • Author(s): Wakayama, T, Sai, Y, Ito, A, Kato, Y, Murakami, Y, Nakashima, E, Tsuji, A, Kitamura, Y, Iseki, S
  • Journal Title: Biol Reprod 76 Pages: 1081-1090
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Altered expression of basement membrane-related molecules in rat brain pericyte, endothelial, and astrocyte cell lines after transforming growth factor-β1 treatment (2007)
  • Author(s): Kose, N, Asashima, T, Muta, M, Iizasa, H, Sai, Y, Terasaki, T, Nakashima, E
  • Journal Title: Drug Metab Pharmacokinet 22 Pages: 255-266
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] c-Met expression in pericytes : implicationsfor atherosclerotic plaque Heagerty A, Canfield A, Alexander M., Hepatocyte growth factor and development (2007)
  • Author(s): Liu, Y, Wilkinson, F, Kirton, J, Jeziorska, M, Iizasa, H, Sai, Y, Nakashima, E
  • Journal Title: J Pathol 212 Pages: 12-19
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Characterization of the uptake mechanism for a novel loop diuretic, M17055, in Caco-2 cells : involvement of organic anion transporting polypeptide (OATP)-B (2007)
  • Author(s): Nishimura, T, Kubo, Y, Kato, Y, Sai, Y, Ogihara, T, Tsuji, A
  • Journal Title: Pharm Res 24 Pages: 90-99
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Organic cation/carnitine transporter octn2 (slc22a5) is responsible for carnitine transport across apical membranes of small intestinal epithelial cells in mouse (2007)
  • Author(s): Kato, Y, Sugiura, M, Sugiura, T, Wakayama, T, Kubo, Y, Kobayashi, D, Sai, Y, Tamai, I, Iseki, S, Tsuji, A
  • Journal Title: Mol Pharmacol 70 Pages: 829-837
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Organic cation/carnitine transporter octn2 (s1c22a5) is responsible for carnitine transport across apical membranes of small intestinal epithelial cells in mouse (2006)
  • Author(s): Kato Y, Sugiura M, Sugiura T, Wakayama T, Kubo Y, Kobayashi D, Sai Y, Tamai I, Iseki S, Tsuji A
  • Journal Title: Mol Pharmacol 70 Pages: 829-837
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Predominant contribution of organic anion transporting polypeptide OATP-B (OATP2B1) to apical uptake of estrone-3-sulfate by human intestinal Caco-2 cells (2006)
  • Author(s): Sai Y, Kaneko Y, Ito S, Kato Y, Tamai I, Artursson P, Tsuji A.
  • Journal Title: Drug Metab Dispos 34 Pages: 1423-1431
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Involvement of organic anion transporting polypeptide OATP-B(OATP2B1) in apical uptake of estrone-3-sulfate by human intestinal Caco-2 cells (2006)
  • Author(s): Sai, Y, Kaneko, Y, Ito, S, Kato, Y, Tamai, I, Artursson, P, Tsuji, A
  • Journal Title: Drug Metab Dispos 34 Pages: 1423-1431
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] DHEASによるラット胎盤トロホブラスト細胞のzidovudine取り込み促進 (2008)
  • Author(s): 関 善彬、西村 友宏、巨勢 典子、崔 吉道、中島 恵美
  • Organizer: 日本薬学会第128年会
  • Place of Presentation: 横浜市
  • Year and Date: 2008-03-26
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Placental transport of erythromycin evaluated using syncytiotrophoblast cell line TR-TBT 18d-1 (2007)
  • Author(s): Sai Y, Ochi K, Kose N, Nishimura T, Nakashima E.
  • Organizer: 4th World Conference on Drug Absorption, Transport and Delivery
  • Place of Presentation: 金沢市
  • Year and Date: 20070620-21
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Placental transport of erythromycin evaluated using syncytiotrophoblast cell line TR-TBT 18d-1 (2007)
  • Author(s): Sai, Y, Ochi, K, Kose, N, Nishimura, T, Nakashima, E
  • Organizer: 4th World Conference on Drug Absorption, Transport and Delivery
  • Place of Presentation: Kanazawa, Japan
  • Year and Date: 20070620-21
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] 胎盤細胞株を用いたヌクレオシド/ヌクレオベース取り込み機構の解明及び薬物との相互作用の検討 (2007)
  • Author(s): 地主 拓也、佐藤 和子、西村 友宏、崔 吉道、中島 恵美
  • Organizer: 第1回次世代を担う若手医療薬科学部会シンポジウム
  • Place of Presentation: 東京
  • Year and Date: 2007-12-15
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] 血液-胎盤関門におけるエリスロマイシン取り込み輸送機構の解析 (2007)
  • Author(s): 高木 彰紀、越智 香織、西村 友宏、崔 吉道、中島 恵美
  • Organizer: 第1回次世代を担う若手医療薬科学部会シンポジウム
  • Place of Presentation: 東京
  • Year and Date: 2007-12-15
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] TR-TBT18d-1及びラット胎盤刷子縁膜小胞を用いたエリスロマイシン取り込み機構解析 (2007)
  • Author(s): 高木 彰紀、越智 香織、西村 友宏、崔 吉道、中島 恵美
  • Organizer: トランスポーター研究会第1回関東部会
  • Place of Presentation: 横浜市
  • Year and Date: 2007-12-10
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] 胎盤関門細胞を用いた薬物、栄養素輸送研究 (2007)
  • Author(s): 崔 吉道、西村 友宏、飯笹 久、巨勢 典子、中島 恵美
  • Organizer: 第29回生体膜と薬物の相互作用シンポジウム
  • Place of Presentation: 仙台市
  • Year and Date: 2007-11-27
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] ラット血液-胎盤関門におけるエリスロマイシンの輸送機構 (2007)
  • Author(s): 高木 彰紀、越智 香織、崔 吉道、西村 友宏、中島 恵美
  • Organizer: 第29回生体膜と薬物の相互作用シンポジウム
  • Place of Presentation: 仙台市
  • Year and Date: 2007-11-27
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] ラット胎盤細胞株を用いたヌクレオシド/ヌクレオベースの母体-胎児血輸送における薬物の相互作用解析 (2007)
  • Author(s): 地主 拓也、崔 吉道、巨勢 典子、佐藤 和子、西村 友宏、中島 恵美
  • Organizer: 第29回生体膜と薬物の相互作用シンポジウム
  • Place of Presentation: 仙台市
  • Year and Date: 2007-11-27
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] 薬物輸送駆動力解明に用いるラット胎盤刷子縁膜小胞の方向性及び精製度評価 (2007)
  • Author(s): 高木 彰紀、西村 友宏、崔 吉道、中島 恵美
  • Organizer: トランスポーター研究会第1回東北部会
  • Place of Presentation: 仙台市
  • Year and Date: 2007-11-25
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Characterization of the placental transport mechanism of erythromycin using syncytiotrophoblast cell line TR-TBT 18d-1 (2007)
  • Author(s): Nishimura T, Sai Y, Ochi K, Kose N, Nakashima E.
  • Organizer: 2007 AAPS Annual Meeting and Exposition
  • Place of Presentation: San Diego,CA,USA
  • Year and Date: 2007-11-15
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] The expression levels of ERM during pregnancy and role of ezrin in localization of GLUT1 and P-glycoprotem in placenta (2007)
  • Author(s): Higuchi K, Wada M, Deguchi M, Horieya S, Kose N, Nishimura T, Sai Y, Wakayama T, Tamura A, Tsukita S, Nakashima E.
  • Organizer: 2007 AAPS Annual Meeting and Exposition
  • Place of Presentation: San Diego,CA,USA
  • Year and Date: 2007-11-15
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Characterization of the placental transport mechanism of erythromycin using syncytiotrophoblast cell line TR-TBT 18d-1 (2007)
  • Author(s): Nishimura, T, Sai, Y, Ochi, K, Kose, N, Nakashima, E
  • Organizer: 2007 AAPS Annual Meeting and Exposition
  • Place of Presentation: San Diego, CA, USA
  • Year and Date: 2007-11-15
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] The expression levels of ERM during pregnancy and role of ezrin in localization of GLUT1 and P-glycoprotein in placenta (2007)
  • Author(s): Higuchi, K, Wada, M, Deguchi, M, Horieya, S, Kose, N, Nishimura, T, Sai, Y, Wakayama, T, Tamura, A, Tsukita, S, Nakashima, E
  • Organizer: 2007 AAPS Annual Meeting and Exposition
  • Place of Presentation: San Diego, CA, USA
  • Year and Date: 2007-11-15
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Involvement of nucleoside transporters in the uptake of anti-HIV drugs,2',3'-dideoxyinosine and 3'-azido-3'-deoxythinudine by a conditionally immortalized syncytiotrophoblast cell line,TR-TBT (2007)
  • Author(s): Nisimura T, Sato K, Sai Y, Kose N, Shimpo S, Nakashima E.
  • Organizer: 8thInternational ISSXMeeting/日本薬物動態学会第22年会
  • Place of Presentation: 仙台市
  • Year and Date: 2007-10-12
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Quantitative analysis of mRNA expression level of ABC transporters in rat primary cultured syncytiotrophoblasts and conditionally immortalized syncytiotrophoblast cell line,TR-TBTs (2007)
  • Author(s): Sai Y, Maeda T, Koizumi T, Kose N, Nishimura T, Nakashima E.
  • Organizer: 8th International ISSX, Meeting/日本薬物動態学会第22年会
  • Place of Presentation: 仙台市
  • Year and Date: 2007-10-12
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Involvement of nucleoside transporters in the uptake of anti-HIV drugs, 2', 3'-dideoxyinosine and 3'-azido- 3'-deoxythimidine by a conditionally immortalized syncytiotrophoblast cell line, TR-TBT (2007)
  • Author(s): Nisimura, T, Sato, K, Sai, Y, Kose, N, Shimpo, S, Nakashima, E
  • Organizer: 8th International ISSX Meeting
  • Place of Presentation: Sendai, Japan
  • Year and Date: 2007-10-12
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Quantitative analysis of mRNA expression level of ABC transporters in rat primary cultured syncytiotrophoblasts and conditionally immortalized syncytiotrophoblast cell line, TR-TBTs (2007)
  • Author(s): Sai, Y, Maeda, T, Koizumi, T, Kose, N, Nishimura, T, Nakashima, E
  • Organizer: 8th International ISSX Meeting
  • Place of Presentation: Sendai, Japan
  • Year and Date: 2007-10-12
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Functional characterization of nucleoside analogues uptake at the placenta and its relevance to drug interaction (2007)
  • Author(s): Sato K, Chisyu T, Sai Y, Nishimura T, Kose N, Nakashima E.
  • Organizer: 日本薬物動態学会ビジョン・シンポジウム
  • Place of Presentation: 東京
  • Year and Date: 2007-07-19
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] mRNA Expression levels of ABC transporter family in highly purified rat syncytiotrophoblasts in the late stage of pregnancy (2007)
  • Author(s): Maeda T, Sai Y, Nishimura T, Kose N, Nakashima E.
  • Organizer: 日本薬物動態学会ビジョン・シンポジウム
  • Place of Presentation: 東京
  • Year and Date: 2007-07-19
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Alteration of expression level of ezrin during pregnancy and influence to the localization of GLUT1 and P-gp in the placenta (2007)
  • Author(s): Higuchi K, Wada M, Deguchi M, Horieya S, Rose N, Nishimura T, Sai Y, Wakayama T, Tamura A, Tsukita S, Nakaclhima E.
  • Organizer: 日本薬物動態学会ビジョン・シンポジウム
  • Place of Presentation: 東京
  • Year and Date: 2007-07-19
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Functional characterization of nucleoside analogues uptake at the placenta and its relevance to drug interaction (2007)
  • Author(s): Sato, K, Chisyu, T, Sai, Y, Nishimura, T, Kose, N, Nakashima, E
  • Organizer: JSSX Vision Symposium 2007
  • Place of Presentation: Tokyo
  • Year and Date: 2007-07-19
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] mRNA Expression levels of ABC transporter family in highly purified rat syncytiotrophoblasts in the late stage of pregnancy (2007)
  • Author(s): Maeda, T, Sai, Y, Nishimura, T, Kose, N, Nakashima, E
  • Organizer: JSSX Vision Symposium 2007
  • Place of Presentation: Tokyo
  • Year and Date: 2007-07-19
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] 「研究成果報告書概要(欧文)」より (2007)
  • Author(s): Higuchi, K, Wada, M, Deguchi, M, Horieya, S, Kose, N, Nishimura, T, Sai, Y, Wakayama, T, Tamura, A, Tsukita, S, Nakashima, E
  • Organizer: JSSX Vision Symposium 2007
  • Place of Presentation: Tokyo
  • Year and Date: 2007-07-19
• [Presentation] Transport mechanism and drug sensitivity of uridine, adenosine and hypoxanthine influx from apical membrane of rat syncytiotrophoblast cell line,TR-TBT 18d-1 (2007)
  • Author(s): Chishu T, Sai Y; Kose N, Sato K, Nishimura T, Nakashima E.
  • Organizer: 4th World Conference on Drug Absorption, Transport and Delivery
  • Place of Presentation: 金沢市
  • Year and Date: 2007-06-21
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Transport mechanism and drug sensitivity of uridine, adenosine and hypoxanthine influx from apical membrane of rat syncytiotrophoblast cell line, TR-TBT 18d-1 (2007)
  • Author(s): Chishu, T, Sai, Y, Kose, N, Sato, K, Nishimura, T, Nakashima, E
  • Organizer: 4th World Conference on Drug Absorption, Transport and Delivery
  • Place of Presentation: Kanazawa, Japan
  • Year and Date: 2007-06-21
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] ラット胎盤におけるezrinの発現変動とP-glycoproteinおよびGLUT1細胞内局在への影響 (2007)
  • Author(s): 崔 吉道、樋口 慧、和田 真実、巨勢 典子、西村 友宏、田村 淳、月田 早智子、若山 友彦、中島 恵美
  • Organizer: 第2回トランスポーター研究会
  • Place of Presentation: 東京
  • Year and Date: 2007-06-09
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] 妊娠経過に伴う胎盤のezrin発現レベルと局在の変動 (2007)
  • Author(s): 樋口 慧、和田 真実、崔 吉道、巨勢 典子、田村 淳、月田 早智子、若山 友彦、中島 恵美
  • Organizer: 日本薬剤学会第22年会
  • Place of Presentation: 大宮市
  • Year and Date: 2007-05-21
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Uptake mechanisms of anti-HIV drugs,2',3'-dideoxyinosine and 3'-azido-3'-deoxythimidine by a conditionally immortalized svncvtiotrobhoblast cell line.TR-TBT (2007)
  • Author(s): Sato K, Sai Y, Kose N, Shimpo S, Nakashima E.
  • Organizer: Pharmaceutical Sciences World Congress 2007
  • Place of Presentation: Amsterdam,Netherlands
  • Year and Date: 2007-04-25
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Uptake mechanisms of anti-HIV drugs, 2', 3'-dideoxyinosine and 3'-azido- 3'-deoxythimidine by a conditionally immortalized syncytiotrophoblast cell line, TR-TBT (2007)
  • Author(s): Sato, K, Sai, Y, Kose, N, Shimpo, S, Nakashima, E
  • Organizer: Pharmaceutical Sciences World Congress 2007
  • Place of Presentation: Amsterdam, Netherlands
  • Year and Date: 2007-04-25
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] ラット胎盤における妊娠経過に伴うezrinの発現量及び局在変動とP-gpの細胞内局在化への関与 (2007)
  • Author(s): 和田 真実、崔 吉道、巨勢 典子、田村 淳、月田 早智子、若山 友彦、上家 潤一、大槻 純男、寺崎 哲也、中島 恵美
  • Organizer: 第127年会日本薬学会
  • Place of Presentation: 富山市
  • Year and Date: 2007-03-29
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] TR-TBT18d-1を用いた血液-胎盤関門におけるErythromycinの輸送機構の解明 (2007)
  • Author(s): 越智 香織、崔 吉道、巨勢 典子、北野 智英、中島 恵美
  • Organizer: 第127年会日本薬学会
  • Place of Presentation: 富山市
  • Year and Date: 2007-03-29
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] ラット血液胎盤関門細胞株におけるzidovudine(AZT)輸送機構の解析 (2007)
  • Author(s): 新保 さりん、崔 吉道、佐藤 和子、地主 拓也、小阿瀬 牧子、巨勢 典子、中島 恵美
  • Organizer: 第127年会日本薬学会
  • Place of Presentation: 富山市
  • Year and Date: 2007-03-29
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] RNA editnngはglycine transporterの機能を変化させるか (2007)
  • Author(s): 阿佐野 霞、崔 吉道、巨勢 典子、飯笹 久、中島 恵美
  • Organizer: 第127年会日本薬学会
  • Place of Presentation: 富山市
  • Year and Date: 2007-03-29
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Ezrin及びCLP36のラット胎盤及びラット胎盤細胞株における細胞内局在性の解明 (2006)
  • Author(s): 崔 吉道、堀江 屋智美、和田 真実、樋口 慧、Lee K-E、飯笹 久、巨勢 典子、若山 友彦、田村 淳、月田 早智子、KangY-S、中島 恵美
  • Organizer: 特定領域「生体膜トランスポートソームの分子機構と生理機能」平成18年度第1回班会議
  • Place of Presentation: 松島
  • Year and Date: 20060927-29
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] RNA editingはTransporter機能を変化させるか (2006)
  • Author(s): 阿佐野 霞、巨勢 典子、飯笹 久、崔 吉道、中島 恵美
  • Organizer: 第1回トランスポーター研究会
  • Place of Presentation: 東京
  • Year and Date: 2006-12-16
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Functional characterization of nucleobase uptake at the placenta and its relevance to the fetoplacental toxicity (2006)
  • Author(s): Sai Y, Chishu T, Koase M, Sato K, Shimpo S, Kose N, Nakashima E.
  • Organizer: 第21回日本薬物動態学会年会
  • Place of Presentation: 東京
  • Year and Date: 2006-11-29
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Functional characterization of nucleobase uptake at the placenta and its relevance to the fetoplacental toxicity (2006)
  • Author(s): Sai, Y, Chishu, T, Koase, M, Sato, K, Shimpo, S, Kose, N, Nakashima, E
  • Organizer: 21st Annual JSSX Meeting
  • Place of Presentation: Tokyo
  • Year and Date: 2006-11-29
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] The functions of transporters and RNA editing (2006)
  • Author(s): Asano K, Kose N, Sai Y, Iizasa H, Nakashima E.
  • Organizer: 2006 AAPS Annual Meeting and Exposition
  • Place of Presentation: San Antonio,TX,USA
  • Year and Date: 2006-11-02
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Apical localization of ezrin in syncytiotrophoblast of rat placenta and sy ncytiotrophoblast cell lines,TR-TBT (2006)
  • Author(s): Wada M, Horieya S, Kose N, Sai Y, Wakayama T, Nakashima E.
  • Organizer: 2006 AAPS Annual Meeting and Exposition
  • Place of Presentation: San Antonio,TX,USA
  • Year and Date: 2006-11-02
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] The functions of transporters and RNA editing (2006)
  • Author(s): Asano, K, Kose, N, Sai, Y, Iizasa, H, Nakashima, E
  • Organizer: 2006 AAPS Annual Meeting and Exposition
  • Place of Presentation: San Antonio, TX, USA
  • Year and Date: 2006-11-02
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Apical localization of ezrin in syncytiotrophoblast of rat placenta and syncytiotrophoblast cell lines, TR-TBT (2006)
  • Author(s): Wada, M, Horieya, S, Kose, N, SaiY, Wakayama, T, Nakashima, E
  • Organizer: 2006 AAPS Annual Meeting and Exposition
  • Place of Presentation: San Antonio, TX, USA
  • Year and Date: 2006-11-02
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] ラット胎盤膜小胞を用いた血液胎盤関門におけるparoxetinei送機構解明 (2006)
  • Author(s): 高木 彰紀、崔 吉道、巨勢 典子、中島 恵美
  • Organizer: 第2回創剤フォーラム若手発表討論会
  • Place of Presentation: 京都市
  • Year and Date: 2006-10-14
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] ラット胎盤細胞株TR-TBT18d-1におけるnucleoside輸送の特徴と薬物との相互作用の解析 (2006)
  • Author(s): 地主 拓也、崔 吉道、巨勢 典子、佐藤 和子、高麗 貴史、中島 恵美
  • Organizer: 第2回創剤フォーラム若手発表討論会
  • Place of Presentation: 京都市
  • Year and Date: 2006-10-13
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] 機能変化に関わるトランスポーターRNA-editingの検索 (2006)
  • Author(s): 阿佐野 霞、田中 紀章、吉川 友理、巨勢 典子、崔 吉道、中島 恵美、飯笹 久
  • Organizer: 第2回創剤フォーラム若手発表討論会
  • Place of Presentation: 京都市
  • Year and Date: 2006-10-13
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] 血液胎盤関門細胞株を用いた輸送研究:ヌクレオシドとカフェイン・ヌクレオシド薬物の相互作用 (2006)
  • Author(s): 新保 さりん、崔 吉道、佐藤 和子、地主 拓也、齊藤 光輝、巨勢 典子、中島 恵美
  • Organizer: 第16回日本医療薬学会年会
  • Place of Presentation: 金沢市
  • Year and Date: 2006-09-30
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Clarification of intracellular localization of CLP36 and ezrin in syncytiotrophoblast and their relevance to polar distribution of transport.ers in the placenta (2006)
  • Author(s): Sai Y, Horieya S, Wada M, Lee K-E, Iizasa H, Kose N, Wakayama T, Tamura A, Tsukita S, Kang Y-S, Nakashima E.
  • Organizer: The 1st Asia Pacific ISSX Meeting
  • Place of Presentation: Jeju,Korea
  • Year and Date: 2006-05-25
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Screening of nucleoside-drug interaction in rat placenta cell line,TR-TBT 18d-1 (2006)
  • Author(s): Chishu T, Sai Y, Kose N, Koma T, Nakashima E.
  • Organizer: The 1st Asia Pacific ISSX Meeting
  • Place of Presentation: Jeju,Korea
  • Year and Date: 2006-05-25
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Influx mechanism of 2',3'-dideoxyinosine (ddI) to a conditionally immortalized syncytioprophoblast cell line,TR-TBT (2006)
  • Author(s): Sato K, Sai Y, Kose N, Koma T, Nakashima E.
  • Organizer: The 1st Asia Pacific ISSX Meeting
  • Place of Presentation: Jeju,Korea
  • Year and Date: 2006-05-25
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Clarification of intracellular localization of CLP36 and ezrin in syncytiotrophoblast and their relevance to polar distribution of transporters in the placenta (2006)
  • Author(s): Sai, Y, Horieya, S, Wada, M, Lee, K-E, Iizasa, H, Kose, N, Wakayama, T, Tamura, A, Tsukita, S, Kang, Y-S, Nakashima, E
  • Organizer: The 1st Asia pacific ISSX Meeting
  • Place of Presentation: Jeju, Korea
  • Year and Date: 2006-05-25
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Screening of nucleoside drug interaction in rat placenta cell line, TR-TBT 18d-1 (2006)
  • Author(s): Chishu, T, Sai, Y, Kose, N, Koma, T, Nakashima, E
  • Organizer: The 1st Asia pacific ISSX Meeting
  • Place of Presentation: Jeju, Korea
  • Year and Date: 2006-05-25
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Influx mechanism of 2', 3'-dideoxyinosine (ddI) to a conditionally immortalized syncytioprophoblast cell line, TR-TBT (2006)
  • Author(s): Sato, K, Sai, Y, Kose, N, Koma, T, Nakashima, E
  • Organizer: The 1st Asia pacific ISSX Meeting
  • Place of Presentation: Jeju, Korea
  • Year and Date: 2006-05-25
  • Description: 「研究成果報告書概要(欧文)」より
• [Book] トランスポーター科学最前線 (2008)
  • Author(s): 崔 吉道
  • Total Pages: 22
  • Publisher: 京都廣川書店
  • Description: 「研究成果報告書概要(和文)」より
• [Remarks] 「研究成果報告書概要(和文)」より
  • URL: http://www.pha.keio.ac.jp/laboratory/laboratory12.html
• [Remarks] 「研究成果報告書概要(和文)」より
  • URL: http://www.keio-yakuzai.jp