2007 Fiscal Year Final Research Report Summary
Individualization of dosage for immunosuppressive drugs based on new biomarkers
| Project/Area Number |
18590155
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Medical pharmacy
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| Research Institution | Musashino University |
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Principal Investigator |
MIHARA Kiyoshi Musashino University, Clinical Pharmaceutics, Associate Professor (00281444)
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| Co-Investigator(Kenkyū-buntansha) |
TANIGAWARA Yusuke Musashino University, School of Medicine, Department of Pharmacy, Professor (30179832)
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| Project Period (FY) |
2006 – 2007
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| Keywords | Organ Transplantation / immunosuppressive drugs / Biomarkers / Rejection / Protein Chip / Pharmacokinetics / Pharmacodynamics / Non-invasive |
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| Research Abstract |
In organ transplantation, needle biopsy of allograft is a standard test for the diagnosis of acute rejection. However, sampling errors, biopsy-associated complications, and a time-consuming process are problem. The aim of this study is to search and identify new biomarkers for allograft rejection and therapeutic effect of immunosuppressive drugs by measuring protein expression profile in peripheral blood and urine. We carried out the rat cardiac transplantation to create three models, isograft, allograft, and allograft with cyclosporine(CsA). The peripheral bloods and urine were collected to perform protein expression profiling using protein chip analysis(surface-enhanced laser desorption/ionization time of flight mass spectrometry ; SELDI-TOF MS). The cation exchange chip revealed that expression of several peptides with molecular weight of 3500, 3600, 4200, and 6500m/z increased in plasma of allograft model. The anion exchange chip revealed that expression of several proteins with molecular weight of 7000, 8000, and 9000m/z increased in plasma of allograft model. In the urine, peptides with molecular weight of 3300, 4400, and 5500m/z increased in allograft model. A comprehensive analysis of the peptides and proteins expression in peripheral blood and urine of rat organ transplantation models results in the discovery of new biomarkers, which could be applied to the therapeutic use.
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Research Products
(13 results)
