Molecular and Cell Biological Studies on Functional Proteins of Skeletal Muscle

2007 Fiscal Year Final Research Report Summary

• Project/Area Number: 18590180
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: General anatomy (including Histology/Embryology)
• Research Institution: Gunma University
• Principal Investigator: YORIFUJI Hiroshi Gunma University, GRADUATE SCHOOL OF MEDICINE, PROFESSOR (00158544)
• Co-Investigator(Kenkyū-buntansha): SATO Mahito GUNMA UNIVERSITY, GRADUATE SCHOOL OF MEDICINE, ASSISTANT PROFESSOR (60375532) ; TAJIKA Yuki GUNMA UNIVERSITY, GRADUATE SCHOOL OF MEDICINE, ASSISTANT PROFESSOR (90400738)
• Project Period (FY): 2006 – 2007
• Keywords: skeletal muscle / VAMP / regeneration / ontogeny / cytoskeletal protein

Research Abstract

Functional proteins of skeletal muscles were studied firm two aspects. One is the turnover of membrane proteins and we concentrated on the control proteins of membrane fusion, especially VAMP (Vesicle Associate Membrane Proteins) family proteins. In mature skeletal muscle of the rat, strong expression of VAMP2 was found in satellite cells. Regenerating muscles which had been injected cardiotoxin showed bright VAMP2 fluorescence at 3 days after injection. During ontogenesis, it appeared in myoblasts of the somites at embyonic day 12 (E12), and remained positive in multi-nucleated myotube at E16. VAMP2 may be involved in myogenesis. In mature skeletal muscles, VAMP1 was found at the nerve terminals of neuromuscular junctions and VAMP3 in endothelial cells of endo- and peri-mysium. The other is an analysis of I vivo function of a cytoskeletal protein of the skeletal muscle. Knockout mice were tried to be generated Heterozygote mice have been obtained and are now under mating to get homozygote animals.

Research Products

• [Journal Article] VAMP2 is expressed in muscle satellite cells and up-regulated during muscle regeneration. (2007)
  • Author(s): Tajika Y, Sato M, Murakami T, Takata K & Yorifuji H.
  • Journal Title: Cell and Tissue Research 328 Pages: 573-581
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] VAMP2 is expressed in muscle satellite cell and up-regulated during imauscle regeneration (2007)
  • Author(s): Tajika Y, Sato M, Murakami T, Takata K & Yorifuji H
  • Journal Title: Cell and Tissue Research 328(3) Pages: 573-581
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] マウス骨格筋組織におけるVAMP5の分布 (2008)
  • Author(s): 多鹿友喜、佐藤真人、村上 徹、依藤 宏
  • Organizer: 第113回日本解剖学会総会・全国学術集会
  • Place of Presentation: 大分大学医学部
  • Year and Date: 2008-03-27
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Expression of VAMP5 in mouse skeletal muscle (2008)
  • Author(s): Yuki Tajika, Mahito Sato, Tohru Murakami, Hiroshi Yorifuji
  • Organizer: 113th Annual Meeting of Japanese Association of Anatomists
  • Place of Presentation: Ohita
  • Year and Date: 2008-03-27
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] 体節におけるVAMP2の発現 (2007)
  • Author(s): 多鹿友喜、佐藤真人、村上 徹、依藤 宏
  • Organizer: 第48回日本組織細胞化学会
  • Place of Presentation: 甲府市総合市民会館
  • Year and Date: 2007-09-28
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Expression of VAMP2 in somite (2007)
  • Author(s): Yuki Tajika, Mahito Sato, Tohru Murakami, Hiroshi Yorifuji
  • Organizer: 48th Annual Meeting of Japan Society if Histochemistry and Cytochemistry
  • Place of Presentation: Kofu
  • Year and Date: 2007-09-28
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] 筋衛星細胞および再生筋におけるVesicle-Associated Membrane Protein-2 (VAMP2)の発現と分布 (2007)
  • Author(s): 多鹿友喜、佐藤真人、村上 徹、依藤 宏
  • Organizer: 第112回日本解剖学会総会・全国学術集会
  • Place of Presentation: 大阪国際会議場
  • Year and Date: 2007-03-29
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Expression of Vesicle-Associated Membrane Protein-2 (VAMP2) in muscle satellite cells and regenerating myotubes (2007)
  • Author(s): Yuki Tajika, Mahito Sato, Tohru Murakami, Hiroshi Yorifuji
  • Organizer: 112th Annual Meeting of Japanese Association of Anatomists
  • Place of Presentation: Osaka
  • Year and Date: 2007-03-29
  • Description: 「研究成果報告書概要(欧文)」より