Morphofunctional studies on the Protein4.1B deficient mouse body

2008 Fiscal Year Final Research Report

• Project/Area Number: 18590181
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: General anatomy (including Histology/Embryology)
• Research Institution: University of Yamanashi
• Principal Investigator: TERADA Nobuo University of Yamanashi, 大学院・医学工学総合研究部, 准教授 (60293461)
• Project Period (FY): 2006 – 2008
• Keywords: 分子形態学 / プロテイン4.1 / 膜骨格 / 免疫組織化学 / 凍結技法 / 細胞接着 / 生体内凍結 / 共焦点ラマン顕微法

Research Abstract

赤血球膜裏側の血管ずり応力に抗する「膜骨格」構成蛋白プロテイン4.1B の臓器組織での局在と病態変化を研究した。4.1B は腎臓でナトリウム-重炭酸イオン共輸送体と、さらに副腎髄質ホルモン分泌細胞で膜貫通細胞接着蛋白TSLC1 と結合していた。また急速凍結による電子顕微鏡3次元観察により膀胱上皮傘細胞のダイナミン蛋白による伸縮機構を明らかにした。さらに生体内凍結技法により血管内ヘモグロビン分子構造変化を瞬時に止めて酸素飽和度の分布をラマン顕微鏡で得た。

Research Products

• [Journal Article] Immunolocalization of serum proteins in xenografted mouse model of human tumor cells by various cryotechniques (2009)
  • Author(s): Bai Y, Ohno N, Terada N, Saitoh S, Nakazawa T, Nakamura N, Katoh R, Ohno S.
  • Journal Title: Histol Histopathol 24(6) Pages: 717-28
  • Peer Reviewed
• [Journal Article] Dispensable role of protein 4.1B/DAL-1 in rodent adrenal medulla regarding generation of pheochromocytoma and plasmalemmal localization of TSLC1 (2009)
  • Author(s): Ohno N, Terada N, Komada M, Saitoh S, Costantini F, Pace V, Germann PG, Weber K, Yamakawa H, Ohara O, Ohno S.
  • Journal Title: 793(3) Pages: 506-15
  • Peer Reviewed
• [Journal Article] Immunolocalization of phospho-Arg-directed protein kinase-substrate in hypoxic kidneys using in vivo cryotechnique (2009)
  • Author(s): Saitoh S, Terada N, Ohno N, Saitoh Y, Soleimani M, Ohno S.
  • Journal Title: Med Mol Morphol 42(1) Pages: 24-31
  • Peer Reviewed
• [Journal Article] Permselectivity of blood follicle barriers in mouse ovaries of the mifepristone-induced polycystic ovary model revealed by in vivo cryotechnique (2008)
  • Author(s): Zhou H, Ohno N, Terada N, Saitoh S, Naito I, Ohno S.
  • Journal Title: Reproduction 136(5) Pages: 599-610
  • Peer Reviewed
• [Journal Article] Application of cryobiopsy to morphological and immunohistochemical analyses of xenografted human lung cancer tissues and functional blood vessels (2008)
  • Author(s): Ohno N, Terada N, Bai Y, Saitoh S, Nakazawa T, Nakamura N, Naito I, Fujii Y,Katoh R, Ohno S.
  • Journal Title: Cancer 113(5) Pages: 1068-79
  • Peer Reviewed
• [Journal Article] Application of "in vivo cryotechnique" to detect erythrocyte oxygen Application of "in vivo cryotechnique" to detect erythrocyte oxygen saturation in frozen mouse tissues with confocal Raman cryomicroscopy (2008)
  • Author(s): Terada N, Ohno N, Saitoh S, Ohno S.
  • Journal Title: J Struct Biol 163(2) Pages: 147-54
  • Peer Reviewed
• [Journal Article] Distribution of immunoglobulin-producing cells in immunized mouse spleens revealed with "in vivo cryotechnique (2008)
  • Author(s): Saitoh S, Terada N, Ohno N, Ohno S.
  • Journal Title: J Immunol Methods 331(1-2) Pages: 114-26
  • Peer Reviewed
• [Journal Article] Ultrastructural study ofhuman glomerular capillary loops with IgA nephropathy using quick-freezing and deep-etching method (2008)
  • Author(s): Sawanobori E, Terada N, Fujii Y, Higashida K, Nakazawa S, Ohno S.
  • Journal Title: Histol Histopathol 23(3) Pages: 297-307
  • Peer Reviewed
• [Journal Article] Extracellular space in mouse cerebellar cortex revealed by in vivo cryotechnique (2007)
  • Author(s): Ohno N, Terada N, Saitoh S, Ohno S.
  • Journal Title: J Comp Neurol 505(3) Pages: 292-301
  • Peer Reviewed
• [Journal Article] Chondrosarcoma with myxoid change : a study using a quick-freezing and deep-etching method (2007)
  • Author(s): Hemmi A, Osaka S, Sumie O, Nemoto N, Ohno N, Terada N, Fujii Y, Ohno S.
  • Journal Title: Ultrastruct Pathol 31(4) Pages: 293-302
  • Peer Reviewed
• [Journal Article] Interaction of membrane skeletal protein, protein 4.1B and p55, and sodium bicarbonate cotransporter1 in mouse renal S1-S2 proximal tubules. (2007)
  • Author(s): Terada N, Ohno N, Saitoh S, Seki G, Komada M, Suzuki T, Yamakawa H, Soleimani M, Ohno S.
  • Journal Title: J Histochem Cytochem 55(12) Pages: 1199-206
  • Peer Reviewed
• [Journal Article] Immunohistochemical detection of hypoxia in mouse liver tissues treated with pimonidazole using "in vivo cryotechnique (2007)
  • Author(s): Terada N, Ohno N, Saitoh S, Ohno S.
  • Journal Title: Histochem Cell Biol 128(3) Pages: 253-61
  • Peer Reviewed
• [Journal Article] Involvement of follicular basement membrane and vascular endothelium in blood follicle barrier formation of mice revealed by 'in vivo cryotechnique' (2007)
  • Author(s): Zhou H, Ohno N, Terada N, Saitoh S, Fujii Y, Ohno S.
  • Journal Title: Reproduction 134(2) Pages: 307-17
  • Peer Reviewed
• [Journal Article] Recent development of in vivo cryotechnique to cryobiopsy for living animals (2007)
  • Author(s): Ohno N, Terada N, Saitoh S, Zhou H, Fujii Y, Ohno S.
  • Journal Title: Histol Histopathol 22(11) Pages: 1281-90
  • Peer Reviewed
• [Journal Article] Conservation and alteration of chromosome territory arrangements in thyroid carcinoma cell nuclei (2007)
  • Author(s): Murata S, Nakazawa T, Ohno N, Terada N, Iwashina M, Mochizuki K, Kondo T, Nakamura N, Yamane T, Iwasa S, Ohno S, Katoh R.
  • Journal Title: Thyroid 17(6) Pages: 489-96
  • Peer Reviewed
• [Journal Article] Raman microscopy of freeze-dried mouse eyeball-slice in conjunction with the "in vivo cryotechnique" (2007)
  • Author(s): Terada N, Ohno N, Saitoh S, Fujii Y, Ohguro H, Ohno S.
  • Journal Title: Microsc Res Tech 70(7) Pages: 634-9
  • Peer Reviewed
• [Journal Article] Electron holography on dynamic motion of secondary electrons around sciatic nerve tissues (2007)
  • Author(s): Shindo D, Kim JJ, Xia W, Kim KH, Ohno N, Fujii Y, Terada N, Ohno S.
  • Journal Title: J Electron Microsc 56(1) Pages: 1-5
  • Peer Reviewed
• [Journal Article] Specific role of the truncated betaIV-spectrin Sigma6 in sodium channel clustering at axon initial segments and nodes of ranvier (2007)
  • Author(s): Uemoto Y, Suzuki S, Terada N, Ohno N, Ohno S, Yamanaka S, Komada M.
  • Journal Title: J Biol Chem 2 ; 282(9) Pages: 6548-55
  • Peer Reviewed
• [Journal Article] Immunohistochemical analyses on serum proteins in nephrons of protein-overload mice by "in vivo cryotechnique". (2007)
  • Author(s): Zhou D, Ohno N, Terada N, Li Z, Morita H, Inui K, Yoshimura A, Ohno S.
  • Journal Title: Histol Histopathol 22(2) Pages: 137-45
  • Peer Reviewed
• [Journal Article] Application of periodic acid-Schiff fluorescence emission for immunohistochemistry of living mouse renal glomeruli by an "in vivo cryotechnique" (2006)
  • Author(s): Li Z, Ohno N, Terada N, Zhou D, Yoshimura A, Ohno S.
  • Journal Title: Arch Histol Cytol 69(3) Pages: 147-61
  • Peer Reviewed
• [Journal Article] Morphological analysis of lamellar structures in mouse type II pneumocytes by quick-freezing and freeze-drying with osmium tetroxide vapor-fixation (2006)
  • Author(s): Yang C, Terada N, Ohno N, Fujii Y, Ohno S.
  • Journal Title: Med Mol Morphol 39(2) Pages: 88-96
  • Peer Reviewed
• [Journal Article] Morphological and histochemical analyses of living mouse livers by new 'cryobiopsy' technique (2006)
  • Author(s): Fujii Y, Ohno N, Li Z, Terada N, Baba T, Ohno S.
  • Journal Title: J Electron Microsc 55(2) Pages: 113-22
  • Peer Reviewed
• [Journal Article] Expression of protein 4.1G in Schwann cells of the peripheral nervous system (2006)
  • Author(s): Ohno N, Terada N, Yamakawa H, Komada M, Ohara O, Trapp BD, Ohno S.
  • Journal Title: J Neurosci Res 84(3) Pages: 568-77
  • Peer Reviewed
• [Journal Article] Application of a quick-freezing and deep-etching method to pathological diagnosis : a case of elastofibroma (2006)
  • Author(s): Hemmi A, Tabata M, Homma T, Ohno N, Terada N, Fujii Y, Ohno S, Nemoto N.
  • Journal Title: J Electron Microsc 55(2) Pages: 89-95
  • Peer Reviewed
• [Journal Article] Immunohistochemical study of NG2 chondroitin sulfate proteoglycan expression in the small and large intestines (2006)
  • Author(s): Terada N, Ohno N, Murata S, Katoh R, Stallcup WB, Ohno S.
  • Journal Title: Histochem Cell Biol 126(4) Pages: 483-90
  • Peer Reviewed
• [Journal Article] Histochemical analyses of living mouse liver under different hemodynamic conditions by "in vivo cryotechnique" (2006)
  • Author(s): Ohno N, Terada N, Ohno S.
  • Journal Title: Histochem Cell Biol 126(3) Pages: 389-98
  • Peer Reviewed
• [Journal Article] Ohno S. Immunolocalization of serum proteins in living mouse glomeruli under various hemodynamic conditions by "in vivo cryotechnique (2006)
  • Author(s): Li Z, Ohno N, Terada N.
  • Journal Title: Histochem Cell Biol 126(3) Pages: 399-406
  • Peer Reviewed
• [Journal Article] 29. Terada N, Ohno N, Fujii Y, Baba T, Ohno S. Dynamic study of intramembranous particles in human fresh erythrocytes using an "in vitro cryotechnique" (2006)
  • Author(s): Terada N, Ohno N, Fujii Y, Baba T, Ohno S.
  • Journal Title: Microsc Res Tech 69(4) Pages: 291-5
  • Peer Reviewed
• [Journal Article] Immunohistochemical detection of phosphorylated rhodopsin in light-exposed retina of living mouse with in vivo cryotechnique (2006)
  • Author(s): Terada N, Ohno N, Ohguro H, Li Z, Ohno S.
  • Journal Title: J Histochem Cytochem 54(4) Pages: 479-86
  • Peer Reviewed
• [Journal Article] Immunohistochemical study of serum albumin in normal and cadmium-treated mouse testis organs by "in vivo cryotechnique" (2006)
  • Author(s): Liao X, Terada N, Ohno N, Li Z, Fujii Y, Baba T, Ohno S.
  • Journal Title: Histol Histopathol 21(3) Pages: 265-72
  • Peer Reviewed
• [Journal Article] Immunohistochemical study of serum albumin in normal and cadmium-treated mouse testis organs by "in vivo cryotechnique" (2006)
  • Author(s): Liao X, Terada N, Ohno N, Li Z, Fujii Y, Baba T, Ohno S.
  • Journal Title: Histol Histopathol 21(1) Pages: 35-40
  • Peer Reviewed
• [Journal Article] Involvement of Dynamin-2 in Formation of Discoid Vesicles in Urinary Bladder Umbrella Cells
  • Author(s): N Terada, N Ohno, S Saitoh, Y Saitoh, Y Fujii, T Kondo, R Katoh, C Chan, S. N. Abraham, and S. Ohno
  • Journal Title: Cell Tissue Res (in press)
  • Peer Reviewed
• [Presentation] Immunohistochemical study of glutamate in mouse retina with "in vivo cryotechnique" in paraffin-embedded sections (2008)
  • Author(s): Terada N, et.al.
  • Organizer: IXth Asia-Pacific Microscopy Conference
  • Place of Presentation: Jeju Korea
  • Year and Date: 2008-11-05
• [Presentation] 膀胱移行上皮内被蓋細胞におけるダイナミン蛋白の免疫組織細胞化学的検討 (2008)
  • Author(s): 寺田信生ら
  • Organizer: 日本組織細胞化学会学術集会
  • Place of Presentation: 長崎
  • Year and Date: 2008-10-06
• [Presentation] "生体内凍結技法"による生きた動物臓器内機能蛋白分子構造と局在の解析法 (2008)
  • Author(s): 寺田信生ら
  • Organizer: 日本臨床分子形態学会
  • Place of Presentation: 福岡
  • Year and Date: 2008-10-03
• [Presentation] Immunolocalization of dynamin-2 around discoid vesicles in umbrella cells of mouse urinary bladder (2008)
  • Author(s): Terada N, et.al
  • Organizer: XIII International Congress of Histochemistry & Cytochemistry
  • Place of Presentation: Gdansk Poland
  • Year and Date: 2008-08-25
• [Presentation] 生体内凍結技法による細胞組織内機能分子局在の解析 (2008)
  • Author(s): 寺田信生ら
  • Organizer: 日本解剖学会全国学術集会
  • Place of Presentation: 大分
  • Year and Date: 2008-03-27
• [Presentation] 生体内凍結試料での蛍光グルコース誘導体と共鳴ラマン波形の蛍光およびラマン顕微鏡による低温下解析 (2007)
  • Author(s): 寺田信生ら
  • Organizer: 日本解剖学会第67 回中部支部学術集会
  • Place of Presentation: 名古屋
  • Year and Date: 2007-10-13
• [Presentation] 生体内凍結技法による低酸素下マウス肝組織におけるピモニダゾール結合蛋白の免疫組織化学的解析 (2007)
  • Author(s): 寺田信生ら
  • Organizer: 第48回日本組織細胞化学会総会
  • Place of Presentation: 甲府
  • Year and Date: 2007-09-28
• [Presentation] マウス中枢神経系におけるProtein4.1(FERM)とPDZドメインを持つKIAA0316蛋白の免疫細胞化学的解析 (2007)
  • Author(s): 寺田信生ら
  • Organizer: 第112回日本解剖学会全国学術集会
  • Place of Presentation: 大阪
  • Year and Date: 2007-03-28
• [Book] In "Neuroacanthocytosis Syndromes II" (ed. Walker RH et.al.) Springer-Verlag (2008)
  • Author(s): Stewart GW, Wilmore SMS, Ohno S, Terada N.
  • Total Pages: 115-132
  • Publisher: Questions of cell shape