2007 Fiscal Year Final Research Report Summary
Nanoscale analysis of signal trans l-ctionbyelecfron microscopy
| Project/Area Number |
18590184
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
General anatomy (including Histology/Embryology)
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| Research Institution | Nagoya University |
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Principal Investigator |
FUJITA Akikazu Nagoya University, Graduate School of Medicine, Associate Professor (60282232)
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| Co-Investigator(Kenkyū-buntansha) |
FUJIMOTO Toyoshi Nagoya University, Graduate School of Medicine, Professor (50115929)
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| Project Period (FY) |
2006 – 2007
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| Keywords | Cell membrane / Lipid / Raft / Glycolipid / Cholesterol / microdomain / special analysis / sienal transduction |
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| Research Abstract |
Lateral heterogeneities in the classical fluid-mosaic model of cell membranes are now envisaged as microdomains or 'rafts' that are enriched in gangliosides, cholesterol and specific membrane proteins. The analyses with single-particle tracking and fluorescent resonance energy transfer techniques have suggested that rafts in normal unstimulated cells are extremely small and may last for less than 1 ms. Electron microscopy (EM) would be expected to have sufficient resolving power to visualize rafts if they are in the nanometer size range, but chemical fixatives that need to be used for conventional sample preparations are unlikely to preserve the in situ localization of membrane molecules, particularly lipids, and may even cause artifactual results. Therefore, we used quick-frozen and freeze-fracture replicas which can physically immobilize molecules in situ and thus minimized the possibility of artifactual perturbation. Using this method, the distribution of gangliosides GM1 and GM3, putative raft molecules in the cell membrane, can be visnalized in the nanometer size range, and they show clusters in normal mouse fibroblasts, which are susceptible to cholesterol depletion and chilling (Mol. Biol. Cell, 18, 2112-2122, 2007)
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[Journal Article] Gangliosides GM1 and GM3 in the living cell membrane form clusters susceptible to cholesterol depletion and chilling2007
Author(s)
Fujita, A, Cheng, J, Hirakawa, M, Furukawa, K, Kusunoki, S, Fujimoto, T
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Journal Title
Mol. Biol. Cell 18
Pages: 2112-2122
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] All-trans-retinol generated by rhodopsin photobleaching induces rapid recruitment of TIP47 to lipid droplets in the retinal pigment epithelium2007
Author(s)
Tsuiki, E, Fujita, A, Ohsald, Y, Cheng, J, Irk, T, Yoshikawa, K, Senoo, H, Mishima, K, Kitaoka, T, Fujimoto, T
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Journal Title
Invest Ophthalmol Vis Sci 48
Pages: 2858-2867
Description
「研究成果報告書概要(欧文)」より
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[Presentation] 細胞内脂質動態と脂質滴2007
Author(s)
大崎雄樹, 程晶磊, 藤田秋一, 藤本豊士
Organizer
日本解剖学会総会
Place of Presentation
相模原市
Year and Date
20070329-31
Description
「研究成果報告書概要(和文)」より
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