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2007 Fiscal Year Final Research Report Summary

A Trial to Establish New Methods for the Evaluation of Bio-affinity of Metal Surfaces by using Cell Culture System

Research Project

Project/Area Number 18590186
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field General anatomy (including Histology/Embryology)
Research InstitutionEhime University

Principal Investigator

KOBAYASHI Naoto  Ehime University, Graduate School of Medicine, Professor (50234836)

Project Period (FY) 2006 – 2007
Keywordsosteoblasts / cell lines / primary culture / clinical materials / titanium / bio-affinity / cell motility / cell adhesion
Research Abstract

The present study aimed to analyze, by using in vitro cell cultures of human osteoblasts, the dynamic cellular behavior on the clinical materials including metal specimens, and to establish new methods for the evaluation of bio-affinity of such materials. The present study has revealed following data. 1) By using a human cell line of osteoblasts (Saos-2), their behavior on the titanium surface was analyzed in the view point of cell spreading and cell motility with a special reference on the surface smoothness (Li C, Gao S, Kobayashi N, et. al., 2006). Furthermore, intracellular signals regulating cell motility were assayed by the same system to show that actin cytoskeleton has a primary role in the cell motility of Saos-2 cells and that the direction of cell motility is thought to be regulated by IP3-kinase signals. 2) By using a primary culture system of human osteoblasts, a new method has been established to evaluate cell motility on clinical materials. When cells were seeded, a glass cover slip was placed in the center of a plastic culture dish and the cover slip was pressed by a weight. After cells were attached on the dish, the cover slip was removed to obtain a cell-free rectangle region, into which cells started to migrate allowing the measurement of cell motility along the time course. After this simple method, cell motility of the primary cultured cells is shown to be analyzed with a significant reproductivity.

  • Research Products

    (18 results)

All 2007 2006

All Journal Article (18 results) (of which Peer Reviewed: 9 results)

  • [Journal Article] The Role of RHO-family small GTPases in regulating cell-cell contacts of cultured podocytes2007

    • Author(s)
      Gao Y, Kobayashi N, et. al.
    • Journal Title

      Cell and Tissue Research 328

      Pages: 391-400

    • Description
      「研究成果報告書概要(和文)」より
    • Peer Reviewed
  • [Journal Article] A double aortic arch with a deformed trachea accompanied by a subaortic left innominate vein2007

    • Author(s)
      Tsujimura R, Kobayashi N, et. al.
    • Journal Title

      Annals of Anatomy 189

      Pages: 197-201

    • Description
      「研究成果報告書概要(和文)」より
    • Peer Reviewed
  • [Journal Article] Localization of prosaposin in rat chochlea2007

    • Author(s)
      Terashita T, Kobayashi N, et. al.
    • Journal Title

      Neuroscience Research 57

      Pages: 372-378

    • Description
      「研究成果報告書概要(和文)」より
    • Peer Reviewed
  • [Journal Article] Distribution of prosaposin in the rat nervous system2007

    • Author(s)
      Hosoda Y, Kobayashi N, et. al.
    • Journal Title

      Cell and Tissue Research 330

      Pages: 197-207

    • Description
      「研究成果報告書概要(和文)」より
    • Peer Reviewed
  • [Journal Article] The Role of RHO-family small GTPases in regulating cell-cell contacts of cultured podocytes2007

    • Author(s)
      Gao, Y, Kobayashi, N, et. al.
    • Journal Title

      Cell and Tissue Research 328

      Pages: 391-400

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] A double aortic arch with a deformed trachea accompanied by a subaortic left innominate vein2007

    • Author(s)
      Tsujimura, R, Kobayashi, N, et. al.
    • Journal Title

      Annals of Anatomy 189

      Pages: 197-201

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Localization of prosaposin in rat chochlea2007

    • Author(s)
      Terashita, T, Kobayashi, N, et. al.
    • Journal Title

      Neuroscience Research 57

      Pages: 372-378

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Distribution of prosaposin in the rat nervous system2007

    • Author(s)
      Hosoda, Y, Kobayashi, N, et. al.
    • Journal Title

      Cell and Tissue Research 330

      Pages: 197-207

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] In vitro assays to detect the adhesion and migration of cultured osteoblastic cells on the titanium surface2006

    • Author(s)
      Li C, Gao S, Kobayashi N, et. al.
    • Journal Title

      Cell and Tissue Research 326

      Pages: 369-375

    • Description
      「研究成果報告書概要(和文)」より
    • Peer Reviewed
  • [Journal Article] Leg dysfunction in a hatched chick model of spina bifida aperta2006

    • Author(s)
      Mominoki K, Kobayashi N, et. al.
    • Journal Title

      Experimental Neurology 193

      Pages: 133-142

    • Description
      「研究成果報告書概要(和文)」より
    • Peer Reviewed
  • [Journal Article] Induction of myasthenia by immunization against muscle-specific kinase2006

    • Author(s)
      Shigemoto K, Kobayashi N, et. al.
    • Journal Title

      Journal of Clinical Investigation 116

      Pages: 1016-1024

    • Description
      「研究成果報告書概要(和文)」より
    • Peer Reviewed
  • [Journal Article] Subdivision of the accessory olfactory bulb in the Japanese common toad,Bufo japonicus,revealed by lectin histochemical analysis2006

    • Author(s)
      Saito S, Kobayashi N, Atoji Y
    • Journal Title

      Anatomy and Embryology 211

      Pages: 395-402

    • Description
      「研究成果報告書概要(和文)」より
    • Peer Reviewed
  • [Journal Article] Coincidence of a right retroaortic circumflex coronary artery and aright-sided aortic arch with a retroesophageal course of the left subclavian artery (arteria lusoria)2006

    • Author(s)
      Kobayashi N, et. al.
    • Journal Title

      Clinical Anatomy 19

      Pages: 354-357

    • Description
      「研究成果報告書概要(和文)」より
    • Peer Reviewed
  • [Journal Article] In vitro assays to detect the adhesion and migration of cultured osteoblastic cells on the titanium surface2006

    • Author(s)
      Li, C, Gao, S, Kobayashi, N, et. al.
    • Journal Title

      Cell and Tissue Research 326

      Pages: 369-375

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Leg dysfunction in a hatuched chick model of spina bifida aperta2006

    • Author(s)
      Mominoki, K, Kobayashi, N, et. al.
    • Journal Title

      Experimental Neurology 193

      Pages: 133-142

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Induction of myasthenia by immunization against muscle-specific kinase2006

    • Author(s)
      Shigemoto, K, Kobayashi, N, et. al.
    • Journal Title

      Journal of Clinical Investigation 116

      Pages: 1016-1024

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Subdivision of the accessory olfactory bulb in the Japanese common toad, Bufo japonicus, revealed by lectin histochemical analysis2006

    • Author(s)
      Saito, S, Kobayashi, N, Atoji, Y
    • Journal Title

      Anatomy and Embryology 211

      Pages: 395-402

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Coincidence of a right retroaortic circumflex coronary artery and a right-sided aortic arch with a retroesophageal course of the left subclavian artery(arteria lusoria)2006

    • Author(s)
      Kobayashi, N, et. al.
    • Journal Title

      Clinical Anatomy 19

      Pages: 354-357

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 2010-02-04  

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