2007 Fiscal Year Final Research Report Summary
Molecular cytochemical analysis of correlation,between nuclear distribution of transcription factors and transcriptional activation or cell differentiation inductivity
| Project/Area Number |
18590189
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
General anatomy (including Histology/Embryology)
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| Research Institution | Nagasaki University |
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Principal Investigator |
IZUMI Shin-ichi Nagasaki University, Graduate School of Biomedical Sciences, Assistant Professor (40264246)
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| Co-Investigator(Kenkyū-buntansha) |
KOMORI Toshihisa Nagasaki University, Graduate School of Biomedical Sciences, Professor (00252677)
MIYAZAKI Toshihiro Nagasaki University, Graduate School of Biomedical Sciences, Assistant Professor (10174161)
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| Project Period (FY) |
2006 – 2007
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| Keywords | Transcription factor / Nucleus / Differentiation / Cytochemistry / Runx2 / Gene / Osteoblast / Transgenic |
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| Research Abstract |
Runx2, a transcription factor plays an important role in cell differentiation of pluripotential undifferentiated mesenchyme into osteoblasts and chondrocytes, and regulates transcription of the target genes by binding to the promoter. Molecular cytochemistry was performed to clarify whether nuclear distribution of Runx2 correlates with the transcription activation and/or DNA binding activity of the transcription factors for the differentiation.
1. When bone morphology protein-2 was introduced to pre-osteoblasts to promote the differentiation by increasing transcription activation of Runx2 gene, Runx2 was strongly stained in the euchromatin area in nuclei.
2. In fetal cartilage of transgenic mice with CoIII-Akt-Tg-GFP, Runx2 appeared in the nuclei of undifferentiated perichondrial cells and proliferating chondrocytes. The staining was increased in the nuclear matrix of mature chondrocytes, and decreased in the differentiated hypertrophy chondrocytes. Nuclear distribution of Runx2 seemed to correlate with DNA binding changes of Runx2 by Akt and chondrocyte differentiation.
3. In tibia of new-born mice of Runx2-Tg, Runx2 and β-catenin appeared in the nuclei of undifferentiated perichondrial cells, and Runx2 was distributed in the nuclear matrix of osteoblasts but not in the osteocytes. In fetal mice of Runx2-/-, neither β-catenin nor osterix was stained in the nuclei of undifferentiated perichondrial cells. Nuclear distribution of Runx2 and β-catenin seemed to be related with the differentiation.
4. When Runx2/GFP were induced in C3H10T1/2 pluripotent undifferentiated fibroblasts, the cytoplasmic processes of the cells were decreased and became short. Immunostaining of actin was decreased and membranous β-catenin aggregated in the cytoplasm. Nuclear matrix distribution of Runx2 seemed to be related with induction of the cell differentiation.
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[Journal Article] Runx2 determines bone maturity and turnover rate in postnatal bone development and is involved in bone loss in estrogen deficiency2007
Author(s)
Maruyama, Z., Yoshida, CA., Furuichi, T., Amizuka, N., Ito, M., Fukuyama, R., Miyazaki, T., Kitaura, H., Nakamura, K., Fujita, T., Kanatani, N., Moriishi, T., Yamana, K., Liu, W., Kawaguchi, H., Nakamura, K., Komori, T
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Journal Title
Developmental Dynamics 236(7)
Pages: 1876-1890
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Diethylstilbestrol increases the density of prolactin cells in male mouse pituitary by inducing proliferation of prolactin cells and transdifferentiation of gonadotropic cells2006
Author(s)
Shukuwa, K., Izumi, S., Hishikawa, Y., Ejima, K., Inoue, S., Muramatsu, M., Ouchi, Y., Kitaoka, T., Koji, T
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Journal Title
Histochemistry and Cell Biology 126
Pages: 111-123
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Histological analysis concerning Runx2 activity on differentiation processes of odontoblasts 48 Suppl.(In Japanese)2006
Author(s)
Miyazaki, T., Eguchi, T., Moriishi, T., Kanatani, N., Izumi, S., Toyosawa, S., Komori, T
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Journal Title
Journal of Oral Biosciences 154(468)
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Cbfβregulates Runx2 function isoform-dependently in postnatal bone development2006
Author(s)
Kanatani, N., Fujita, T., Fukuyama, R., Liu, W., Yoshida, CA., Moriishi, T., Yamana, K., Miyazaki, T., Toyosawa, S., Koji, T
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Journal Title
Developmental Biology 296
Pages: 48-61
Description
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[Presentation] Over-expression of Runx2 in mesenchymal cells enhanced bone formation but inhibited cartilage formation and limb development2007
Author(s)
Yoshida, CA., Maeno, T., Kanatani, N., Izumi, S., Komori, T
Organizer
The 29th Annual Meeting of ASBMR(the American Society of Bone and Mineral Metabolism)
Place of Presentation
Honolulu, USA
Year and Date
20070916-20
Description
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[Presentation] Histological analysis concerning Runx2 activity on differentiation processes of odontoblasts(In Japanese)2006
Author(s)
Miyazaki, T., Eguchi, T., Moriishi, T., Kanatani, N., Izumi, S., Toyosawa, S., Komori, T
Organizer
The 48th annual meeting of society of oral basic medicine
Place of Presentation
Yokohama
Year and Date
20060921-23
Description
「研究成果報告書概要(欧文)」より
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