Functional analysis of the voltage-dependent calcium channel β subunit in cardiac contraction

2008 Fiscal Year Self-evaluation Report

• Project/Area Number: 18590202
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: General physiology
• Research Institution: Akita University
• Principal Investigator: MURAKAMI Manabu Akita University, 医学部, 准教授 (80302090)
• Project Period (FY): 2006 – 2009
• Keywords: カルシウム / 交感神経 / チャネル / TRP / 高血圧

Research Abstract

心筋収縮における電位依存性カルシウムチャネル, 特にβサブユニットの重要性を,遺伝子改変マウスを用いて明らかにするのが, 研究の目的である.
電位依存性カルシウムチャネルのβサブユニット遺伝子改変マウスを作製し, 遺伝子の機能を解析する. その他, チャネル遺伝子クローニング, カルシウムチャネル遺伝子変異マウスの解析を循環器系中心に広く展開する.

Research Products

• [Journal Article] Modified sympathetic nerve system activity with over-expression of the voltage-dependent calcium channel β3 subunit (2008)
  • Author(s): Murakami, M., Ohba, T., Xu, F., Satoh, E., Miyoshi, I., Suzuki, T., Takahashi, Y., Takahashi, E., Watanabe, Ono, K., Sasano, H., Kasai, N., Ito, H. and Iijima, T.
  • Journal Title: J. Biol. Chem 283 Pages: 24554-24560
  • Peer Reviewed
• [Journal Article] TRP channel and cardiovascular disease (2008)
  • Author(s): Watanabe H, Murakami M, Ohba T, Takahashi and YIto H.
  • Journal Title: Pharmacology & Therapeutics 118 Pages: 337-351
  • Peer Reviewed
• [Journal Article] Modified sympathetic regulation in N-type calcium channel null-mouse (2007)
  • Author(s): Murakami, M., Ohba, T., Wu, T-W., Fujisawa, S., Suzuki, T., Takahashi, Y., Takahashi, E., Watanabe, H., Miyoshi, I., Ono, K., Sasano, H., Ito, H. and Iijima T.
  • Journal Title: Biochemical & Biophysical Research Communications 354 Pages: 1016-1020
  • Peer Reviewed
• [Journal Article] Involvement of transient receptor potential canonical 1 (TRPC1) in Angiotensin II-induced vascular smooth muscle cell hypertrophy (2007)
  • Author(s): Takahashi, Y., Watanabe, H., Murakami, M., Ohba, T., Radovanovic, M., Ono, K., Iijima, T. and Ito, H.
  • Journal Title: Atheroscrelosis 195 Pages: 287-296
  • Peer Reviewed
• [Journal Article] Up-regulation of TRPC1 in the Development of Cardiac Hypertrophy (2007)
  • Author(s): Ohba, T., Watanabe, H., Murakami, M., Takahashi, Y., Iino, K., Kuromitsu, S., Mori, Y., Ono, K., Iijima T. and Ito, H.
  • Journal Title: Journal of Molecular and Cellular Cardiology 42 Pages: 498-507
  • Peer Reviewed
• [Presentation] Modified sympathetic nerve system activity with over-expression of the voltage-dependent calcium channel β3 subunit (2008)
  • Author(s): M. Murakami
  • Organizer: 38^<st> Annual Meeting Washington D.C.
  • Place of Presentation: Society for Neuroscience
  • Year and Date: 20081100
• [Presentation] Anti-proliferative Effect of amlodipine on Human Coronary Artery Smooth Muscle Cells (2008)
  • Author(s): T. Ohba, H. Watanabe, M. Murakami, H. Hasegawa and H. Ito
  • Organizer: 第12回日本心不全学会学術集会
  • Place of Presentation: 東京
  • Year and Date: 20081000
• [Presentation] ヒト冠動脈平滑筋におけるアムロジピン抗増殖作用の新規メカニズム (2008)
  • Author(s): 大場貴喜, 村上学, 藤澤進, 渡邊博之, 尾野恭一, 伊藤宏, 飯島俊彦
  • Organizer: 第81回日本薬理学会年会
  • Place of Presentation: 横浜
  • Year and Date: 20080300
• [Presentation] N型カルシウムチャネル欠損マウスにおける交感神経制御機構 (2008)
  • Author(s): 村上学, 大場貴喜, 藤澤進, 渡邊博之, 尾野恭一, 伊藤宏, 飯島俊彦
  • Organizer: 第81回日本薬理学会年会
  • Place of Presentation: 横浜
  • Year and Date: 20080300
• [Presentation] Cloning of novel isoforms of murine cardiac calcium channel α1 subunit and their different binding affinities with β2 subunit (2007)
  • Author(s): M. Murakami
  • Organizer: 37^<st> Annual Meeting San Diego
  • Place of Presentation: Society for Neuroscience
  • Year and Date: 20071100