Functional analysis of the voltage-dependent calcium channel β subunit in cardiac myocyte

2009 Fiscal Year Final Research Report

• Project/Area Number: 18590202
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: General physiology
• Research Institution: Akita University
• Principal Investigator: MURAKAMI Manabu Akita University, 大学院・医学系研究科, 准教授 (80302090)
• Co-Investigator(Kenkyū-buntansha): ONO Kyoichi 秋田大学, 大学院・医学系研究科, 教授 (70185635) ; OHBA Takayoshi 秋田大学, 大学院・医学系研究科, 助教 (80431625) ; IIJIMA Toshihiko 秋田大学, 名誉教授 (30004724)
• Project Period (FY): 2006 – 2009
• Keywords: 生体膜 / チャネル / トランスポーター / 能動輸送

Research Abstract

Using gene-manipulated mice, we evaluated importance of voltage-dependent calcium channel β subunit in sympathetic nerve regulation system.
It is well known, that cardiac myocytes are regulated by sympathetic nerve system through voltage-dependent calcium channel. To evaluate the importance of the voltage-dependent calcium channel β subunit in the sympathetic nerve control, we made null-mutant and overexpression transgenic mice of β subunit. The null-mutant showed significantly decreased sympathetic nerve tonus, while the overexpression mice revealed increased nerve tonus, indicating the importance of the β subunit in the sympathetic nerve control system.

Research Products

• [Journal Article] Behavioral and neurochemical characterization of mice deficient in the N-type Ca^<2+> channel α1B subunit (2010)
  • Author(s): Nakagawasai, O., Onogi, H., Mitazaki, S., Sato, A., Watanabe, K., Saito, H., Murai, S., Nakaya, S., Murakami, M., Takahashi, E., Tan-No, K., Takeshi Tadano, T.
  • Journal Title: Behavioural Brain Research (in press)
• [Journal Article] Decreased calcium channel currents and facilitated epinephrine release in the Ca^<2+> channel β3 subunit-null mice (2010)
  • Author(s): Ohta T, Ohba T, Suzuki T, Watanabe H, Sasano H, Murakami M.
  • Journal Title: Biochemical & Biophysical Research Communications (in press)
• [Journal Article] Essential role of STIM1 in the development of cardiomyocyte hypertrophy (2009)
  • Author(s): Ohba, T., Watanabe, H., Murakami, M., Sato, T., Ono, K., Ito, H.
  • Journal Title: Biochemical & Biophysical Research Communications 389 Pages: 172-176
• [Journal Article] Molecular and Electrical Remodeling of L- and T- type Ca^<2+> Channels in Rat Right Atrium with Monocrotaline-Induced Pulmonary Hypertension (2009)
  • Author(s): Koyama, T., Ono, K., Watanabe, H., Ohba, T., Murakami, M., Iino, K., Ito H.
  • Journal Title: Circ.J. 73 Pages: 256-263
• [Journal Article] The pathological role of TRP channels in heart disease (2009)
  • Author(s): Watanabe, H., Murakami, M., Ohba, T., Ono, K., Ito, H.
  • Journal Title: Circulation Journal (in press)
• [Journal Article] Amlodipine inhibits cell proliferation via PKD1-related pathway (2008)
  • Author(s): Ohba, T., Watanabe, H., Murakami, M., Radovanovic, M., Iino, K., Ishida, M., Tosa, S., Ono, K., Ito, H.
  • Journal Title: Biochemical & Biophysical Research Communications 369 Pages: 376-381
• [Journal Article] TRP channel and cardiovascular disease (2008)
  • Author(s): Watanabe H, Murakami M, Ohba T, Takahashi Y, Ito H.
  • Journal Title: Pharmacology & Therapeutics 118 Pages: 337-351
• [Journal Article] Modified sympathetic nerve system activity with over-expression of the voltage-dependent calcium channel β3 subunit (2008)
  • Author(s): Murakami, M., Ohba, T., Xu, F., Satoh, E., Miyoshi, I., Suzuki, T., Takahashi, Y., Takahashi, E., Watanabe, Ono, K., Sasano, H., Kasai, N., Ito, H., Iijima, T.
  • Journal Title: J.Biol.Chem. 283 Pages: 24554-24560
• [Journal Article] Up-regulation of TRPC1 in the Development of Cardiac Hypertrophy (2007)
  • Author(s): Ohba, T., Watanabe, H., Murakami, M., Takahashi, Y., Iino, K., Kuromitsu, S., Mori, Y., Ono, K., Iijima T., Ito, H.
  • Journal Title: Journal of Molecular and Cellular Cardiology 42 Pages: 498-507
• [Journal Article] Involvement of transient receptor potential canonical 1 (TRPC1) in Angiotensin II-induced vascular smooth muscle cell hypertrophy (2007)
  • Author(s): Takahashi, Y., Watanabe, H., Murakami, M., Ohba, T., Radovanovic, M., Ono, K., Iijima, T., Ito, H.
  • Journal Title: Atheroscrelosis 195 Pages: 287-296
• [Journal Article] Modified sympathetic regulation in N-type calcium channel null-mouse (2007)
  • Author(s): Murakami, M., Ohba, T., Wu, T-W., Fujisawa, S., Suzuki, T., Takahashi, Y., Takahashi, E., Watanabe, H., Miyoshi, I., Ono, K., Sasano, H., Ito, H. Iijima T.
  • Journal Title: Biochemical & Biophysical Research Communications 354 Pages: 1016-1020
• [Journal Article] Involvement of the calcium channel beta3 subunit in olfactory signal transduction (2007)
  • Author(s): Shiraiwa, S., Kashiwayanagi, M., Iijima, T., Murakami, M.
  • Journal Title: Biochemical & Biophysical Research Communications 355 Pages: 1019-1024
• [Journal Article] Essential role of the N-terminus of murine Orail in store-operated Ca^<2+> entry (2007)
  • Author(s): Takahashi, Y., Murakami, M., Watanabe, H., Hasegawa, H., Ohba, T., Munehisa, Y., Nobori, K., Ono, K., Iijima, T., Ito, H.
  • Journal Title: Biochemical & Biophysical Research Communications 356 Pages: 45-52
• [Journal Article] Modified behavioral characteristics following ablation of the voltage-dependent calcium channel β3 subunit (2007)
  • Author(s): Murakami, M., Nakagawasai, O., Yanai, K., Nunoki, K., Tan-No, K., Tadano T. Iijima, T.
  • Journal Title: Brain Research 1160 Pages: 102-112
• [Journal Article] Functional role of stromal interaction molecule 1 (STIM1) in vascular smooth muscle cells (2007)
  • Author(s): Takahashi, Y., Watanabe, H., Murakami, M., Ono, K., Munehisa, Y., Koyama, T., Nobori, K., Iijima, T., Ito, H.
  • Journal Title: Biochemical & Biophysical Research Communications 361 Pages: 934-940
• [Journal Article] Genomic organization and expression of murine calcium channel subunit α1C. Saito Ho-on Kai Museum of Natural History (2007)
  • Author(s): Murakami, M., Ohba, T., Ono, K., Watanabe, H., Ito, H., Iijima, T.
  • Journal Title: Research Bulletin 72 Pages: 1-10
• [Journal Article] Decreases in pheromonal responses at the accessory olfactory bulb of mice with a deficiency of the α1B or β3 subunits of voltage-dependent Ca^<2+>-channels (2006)
  • Author(s): Murakami, M., Matsui, H., Shiraiwa, T., Suzuki, T., Sasano, H., Takahashi E., Kashiwayanagi, M.
  • Journal Title: Biological and Pharmaceutical Bulletin 29 Pages: 437-442
• [Journal Article] Involvement of Voltage-Dependent Ca Channel beta3 Subunit in the Autonomic Control of Heart Rate Variability (2006)
  • Author(s): Wu TW, Ono K, Murakami M, Iijima T.
  • Journal Title: Pharmacology 76 Pages: 170-179
• [Journal Article] Identification of a cardiac isoform of the murine calcium channel α1C (Ca_V1.2-a) subunit and its preferential binding with the β2 subunit (2006)
  • Author(s): Murakami, M., Ohba, T., Takahashi, Y., Watanabe, H., Miyoshi, I., Nakayama, S., Ono, K., Ito, H., Iijima T.
  • Journal Title: Journal of Molecular and Cellular Cardiology 41 Pages: 115-125
• [Journal Article] Regulatory role of neuron-restrictive silencing factor in the expression of TRPC1 (2006)
  • Author(s): Ohba, T., Watanabe, H., Takahashi, Y., Miyoshi, I., Nakayama, S., Satoh, E., Iino, K., Mori, Y., Kuromitsu, S., Imagawa, K., Saito, Y., Iijima, T., Ito, H., Murakami, M.
  • Journal Title: Biochemical & Biophysical Research Communications 351 Pages: 764-770
• [Presentation] Modified autonomic regulation in mice with a P/Q-type calcium channel mutation (2009)
  • Author(s): Murakami M, Ohba T, Ono K
  • Organizer: Society for Neuroscience, 39th Annual Meeting
  • Place of Presentation: Chicago, USA
  • Year and Date: 20090000
• [Presentation] Modified sympathetic nerve system activity with over-expression of the voltage-dependent calcium channel β3 subunit (2009)
  • Author(s): Murakami M, Ohba T, Ono K
  • Organizer: Congress of Physiological Sciences
  • Place of Presentation: Kyoto, Japan
  • Year and Date: 20090000
• [Presentation] Modified sympathetic nerve system activity with over-expression of the voltage-dependent calcium channel β3 subunit (2008)
  • Author(s): Murakami M, Ohba T, Ono K
  • Organizer: Society for Neuroscience, 38th Annual Meeting
  • Place of Presentation: Washington DC, USA
  • Year and Date: 20080000
• [Presentation] Cloning of novel isoforms of murine cardiac calcium channel α1 subunit and their different binding affinities with β2 subunit (2007)
  • Author(s): Murakami M, Ohba T, Takahashi Y, Ono K, Watanabe H, Ito H, Iijima T
  • Organizer: Society for Neuroscience, 37th Annual Meeting
  • Place of Presentation: San Diego, USA
  • Year and Date: 20070000
• [Remarks] ホームページ等