2007 Fiscal Year Final Research Report Summary
Study on the molecular basis of the response of the digestive tract evoked by nutrients/ food factors
| Project/Area Number |
18590220
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Environmental physiology (including Physical medicine and Nutritional physiology)
|
|---|
| Research Institution | University of Shizuoka |
|---|
Principal Investigator |
GODA Toshinao University of Shizuoka, School of Food and Nutritional Sciences, Department of Nutrition and Life Sciences, Professor (70195923)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
MOCHIZUKI Kazuki University of Shizuoka, School of Food and Nutritional Sciences, Department of Nutrition and Life Sciences, Assistant Professor (80423838)
|
|---|
| Project Period (FY) |
2006 – 2007
|
| Keywords | gene / transcriptional regulation / nutrition / nutrient signal / chromatin / histone code / small intestine / microarray |
|---|
| Research Abstract |
1. Transcriptional controls through TR, GR and RXR in the small intestine: Ligands of glucocorticoid hormone receptor GR and thyroid hormone receptor TR synergistically enhanced the gene expression of fructose transporter GLUTS in human intestinal cell line, Caco-2, which was accompanied by increases in the binding of TR, GR, coactivators and acetylated histones H3 and H4 on the promoter region of GLUTS gene. Dephosphorylation of TR and GR by MAP kinase inhibitor caused an enhancement of TR transcriptional activity and GR nuclear translocation. Transfection assay in C2BBe1 cell showed that retinal dehydrogenase detected in the cell was sufficient to convert 9-cis retinal to the ligand for RXR.
2. Transcriptional controls of carbohydrate digestion/absorption-related genes through a carbohydrate signal: Microarray analysis of the gene expression in rats fed a high carbohydrate diet showed that 52 transcription/chromatin-related genes were up-regulated in jejunum. In mice fed a high carbohydrate diet, the expression of sucrase-isomaltase (SI) gene was enhanced in jejunum, where the bindings of acetylatedhistones H3 and H4 and that of putative transcription factors, i.e., Cdx-2 and HNF-1, were enhanced on the proximal region of SI gene. Feeding fructose solution in mice caused an increase in the binding of acetylated histones H3 and H4 on the proximal region of SI gene.
3. Regulation of the expression of target genes through fatty acids signal: Feeding caprylic acid or oleic acid in weanling rats caused a parallel increase in the gene expression of PPAR, CBP/p300 and their target genes, i.e., L-FABP and CRBPII in the jejunum, When CRBPII gene is abruptly induced during the perinatal period in the small intestine of rats, the binding of RXR and coactivators as well as that of acetylated histones H3 and H4 was enhanced on the proximal region of CRBPII gene.
|
Research Products
(61 results)
-
-
-
-
-
-
-
-
-
-
-
[Journal Article] Fatty acids in component of milk enhance the expression of the cAMP-response-element-binding-protein-binding-protein (CBP)/p300 gene in developing rats2008
Author(s)
Mochizuki, K., Kawai, H., Mochizuki, H., Shimada, M Takase, S., Goda, T
-
Journal Title
Br. J Nutr 99
Pages: 481-486
Description
「研究成果報告書概要(欧文)」より
-
-
-
-
[Journal Article] Distribution and dietary induction of cellular retinol-binding protein type II along the villus-crypt axis of the rat jejunum2008
Author(s)
Ogura, Y., Yasutake, H., Mochizuki, K., Yoshikawa, S., Suruga, K., Sugiyama, H., Takase, S., Goda, T
-
Journal Title
J. Nutr. Sci. Vitaminol 54
Pages: 130-135
Description
「研究成果報告書概要(欧文)」より
-
-
[Journal Article] Higher expression of jejunal LPH gene in rats fed the high-carbohydrate/low-fat diet compared with in those fed the low-carbohydrate/high-fat diet is associated with in vitro binding of Cdx-2 in nuclear proteins to its promoter regions2008
Author(s)
Tanaka, T., Suzuki, A., Kuranuki, S., Mochizuki, K., Suruga, K., Takase, S., Goda, T
-
Journal Title
Description
「研究成果報告書概要(欧文)」より
-
-
-
-
-
-
-
-
-
-
[Journal Article] The critical period for thyroid hormone responsiveness through thyroid hormone receptor isoform alpha-1 in the postnatal small intestine2007
Author(s)
Mochizuki, K., Yagi, R., Sakaguchi, N., Mochizuki, H., Takabe, S., Kuranuki, S., Suzuki, T., Shimada, M., Goda, T
-
Journal Title
Biochim. Biophys. Acta 1770
Pages: 609-616
Description
「研究成果報告書概要(欧文)」より
-
-
-
-
-
[Journal Article] Possible role of fatty acids in milk as the regulator of the expression of cytosolic binding proteins for fatty acid and vitamin A through PPAR a in developing rats2007
Author(s)
Mochizuki, K., Mochizuki, H., Kawai, H., Ogura, Y., Shimada, M., Takase, S., Goda, T
-
Journal Title
J. Nutr. Sci. Vitaminol 53
Pages: 515-521
Description
「研究成果報告書概要(欧文)」より
-
-
-
-
[Journal Article] Selectivity of fatty acid ligands for PPAR a which correlates both with binding to cis-element and DNA binding-independent transactivity in Caco-2 cells.2006
Author(s)
Mochizuki, K., Suruga, K., Fukami, H., Kiso, Y., Takase, S. , Goda, T
-
Journal Title
Life Sci 80
Pages: 140-145
Description
「研究成果報告書概要(欧文)」より
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
[Presentation] Regulation of the expression of genes related to vitamin A metabolism through 9-cis- and all-trans-retinoic in the small intestine2006
Author(s)
Ogura, Y., Kajigaya, K., Mochizuki, K., Suruga, K., Takase, S., Goda, T., Mochizuki and Goda, T
Organizer
60th Annual Meeting of Japanese Society of Nutrition and Food Science
Place of Presentation
Shizuoka
Year and Date
20060500
Description
「研究成果報告書概要(欧文)」より
-
-
-
[Presentation] Effects of 9-cis- and all-trans-retinoids on C2BBe1 cells2006
Author(s)
Ogura, Y., Kajigaya, K., Mochizuki, K., Suruga, K., Takase, S., Kagechika, H., Goda, T
Organizer
58th Annual Meeting of the Vitamin Society of Japan
Place of Presentation
Tokushima
Year and Date
20060500
Description
「研究成果報告書概要(欧文)」より
-
-
