2007 Fiscal Year Final Research Report Summary
Molecular regulation of human small intestinal absorption and metabolism of vitamin A.
| Project/Area Number |
18590222
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Environmental physiology (including Physical medicine and Nutritional physiology)
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| Research Institution | Siebold University of Nagasaki |
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Principal Investigator |
SURUGA Kazuhito Siebold University of Nagasaki, Faculty of Nursing and Nutritional, Associate Professor (70315852)
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| Project Period (FY) |
2006 – 2007
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| Keywords | Vitamin A / β-carotene / HNF-4 / HNF-1 / small intestine / absorption / phosphorylation |
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| Research Abstract |
Human intestinal-like cell line C2BBe1 cells expressed higher levels of BCMO1, CRBPII and other vitamin A-related genes' transcripts than parental Caco-2 cells. Human CRBPII gene transcription was synergistically stimulated by HNF-4α and intestinal-specific transcription factors (CDX2 and GATAs). Human BCMO1 gene transcription was competitively regulated by HNF-4α and HNF-1α on the same distal promoter element. Moreover, PMA significantly decreased CRBPII and BCMO1 gene expression levels through PKC-ERK-mediated down-regulation of nuclear HNF-4α and HNF-1 levels in C2BBe1 cells. These transcription factors are also involved in the differentiation-induced vitamin A and β-carotene absorption and metabolism in the human small intestinal cells. In conclusion, HNF-4α and/or HNF-1α may be pivotal transcription factors involving in the bioavailability and homeostasis of human small intestinal vitamin A and β-carotene.
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[Journal Article] The effects of all-trans and 9-cis retinoids in C2BBe1 cells.2007
Author(s)
Ogura, Y., Mochizuki, K., Kajigaya, K., Suruga, K., Takase, S., Kagechika, H., Goda, T
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Journal Title
Vitamins 81
Pages: 483-488
Description
「研究成果報告書概要(欧文)」より
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