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2007 Fiscal Year Final Research Report Summary

Development of in vivo imaging system to examine the muscle regeneration

Research Project

Project/Area Number 18590263
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field General medical chemistry
Research InstitutionKyoto University

Principal Investigator

KURISAKI Tomohiro  Kyoto University, Institute for frontier Medical Sciences, Assistant (90311422)

Project Period (FY) 2006 – 2007
Keywordsskeletal muscle / regeneration / in vivo imaging / regeneration of skeletal muscle / muscular dystrophy
Research Abstract

This research has been focused on establishment of in vivo imaging system to examine the process of muscle regeneration. Three different approach has been taken. First, using LacZ transgenic mice, I prepared to integrate fluorescent microscope system. With this system, We succeeded to detect terminal Schwann cells on skeletal muscle in ligving animal. We tried to observe regeneration of sternomastoid muscle, cremaster muscle, gracilis muscle, latissimus dorsi muscle and flexor hallucis brevis. We also screened fluorescent markers and methods of muscle injury. Second, using Zebrafish embryo, we traced the muscle precurcer cells by confocal laser microscopy. Third, we made and screened monoclonal antibodies to detect subpopulation of muscle precurcer cells at specific stage. Then we could identify a couple of novel membrane proteins. Based on the data above, we keep trying to establish in vivo imaging system to examine the process of muscle regeneration.

  • Research Products

    (2 results)

All 2007

All Presentation (2 results)

  • [Presentation] 筋形成に関与する新規遺伝子の探索2007

    • Author(s)
      栗崎 知浩
    • Organizer
      筋ジストロフィーおよびその関連する疾患の病態生理の解明と治療薬物の開発に関する研究
    • Place of Presentation
      東京
    • Year and Date
      2007-12-08
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] Screening of novel genes involved in myogenesis2007

    • Author(s)
      Tomohiro, Kurisaki
    • Organizer
      Musclular dystrophy and related disorder, Pathology and Therapy
    • Place of Presentation
      Tokyo
    • Year and Date
      2007-12-08
    • Description
      「研究成果報告書概要(欧文)」より

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Published: 2010-02-04  

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