2007 Fiscal Year Final Research Report Summary

Development of in vivo imaging system to examine the muscle regeneration

Research Project

Project/Area Number	18590263
Research Category	Grant-in-Aid for Scientific Research (C)
Allocation Type	Single-year Grants
Section	一般
Research Field	General medical chemistry
Research Institution	Kyoto University
Principal Investigator	KURISAKI Tomohiro Kyoto University, Institute for frontier Medical Sciences, Assistant (90311422)
Project Period (FY)	2006 – 2007
Keywords	skeletal muscle / regeneration / in vivo imaging / regeneration of skeletal muscle / muscular dystrophy
Research Abstract	This research has been focused on establishment of in vivo imaging system to examine the process of muscle regeneration. Three different approach has been taken. First, using LacZ transgenic mice, I prepared to integrate fluorescent microscope system. With this system, We succeeded to detect terminal Schwann cells on skeletal muscle in ligving animal. We tried to observe regeneration of sternomastoid muscle, cremaster muscle, gracilis muscle, latissimus dorsi muscle and flexor hallucis brevis. We also screened fluorescent markers and methods of muscle injury. Second, using Zebrafish embryo, we traced the muscle precurcer cells by confocal laser microscopy. Third, we made and screened monoclonal antibodies to detect subpopulation of muscle precurcer cells at specific stage. Then we could identify a couple of novel membrane proteins. Based on the data above, we keep trying to establish in vivo imaging system to examine the process of muscle regeneration.