2007 Fiscal Year Final Research Report Summary
Analysis for factors determining cancer stem cells ; aiming for their visualization
| Project/Area Number |
18590329
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Human pathology
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| Research Institution | Osaka University |
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Principal Investigator |
MORII Eiichi Osaka University, Department of Pathology, Associate Professor (10283772)
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| Project Period (FY) |
2006 – 2007
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| Keywords | cancer stem cells / pathology / immunohistochemitry |
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| Research Abstract |
To aim for visualization of cancer stem cells, we first determined the molecules highly expressed in the side population of cancer cell lines, in which cancer stem cells are known to be contained. We isolated several candidates of molecules highly eapiessod in side population of breast cancer cell line MCIF7, such as CD55. Cells with CD55-high character expressed anti-apoptotic molecules abundantly, and formed colonies in vitro more efficiently. In the transplantation to NOD/Scid mice, cells with CD55-high character were more tumorigenic than cells with CD55-low character. In addition, we found breast cancer cases with ample CD55-high tumor cells showed poor prognosis as compared to the caces with scarce CD55-high tumor cells. These showed that the quantity of cancer stem cells might determine the prognosis of tumors. Besides CD55, we found several molecules, such as Notch2, would be a marker for tumorigenic cells of lymphoma cell line.
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