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2007 Fiscal Year Final Research Report Summary

Fumctional analysis of AEPP in skeletal muscle

Research Project

Project/Area Number 18590375
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Experimental pathology
Research InstitutionOita University

Principal Investigator

MORIYAMA Masatsugu  Oita University, Department of medicine, Professor (90239707)

Co-Investigator(Kenkyū-buntansha) MATSUURA Keiko  Oita University, Department of medicine, Associate Professor (00291542)
HIJIYA Naoki  Oita University, Department, ofmedicine, Assistant Professor (80305036)
UCHIDA Tomohisa  Oita University, Department of medicine, Assistant Professor (70381035)
Project Period (FY) 2006 – 2007
KeywordsARPP / CARP / muscle injury / Skeletal muscle / MARP / Nuclear translocation / Sarcomere / I band
Research Abstract

Ankyrin-repeat protein with a PEST motif and a proline-rich region(Arpp), also designated as Ankrd2, is a member of the muscle ankyrin repeat proteins (MARPs), which have been proposed to be involved in muscle stress response pathways. Arpp/Ankrd2 is localized mainly in the I-band of striated muscle. However, it has recently been reported that Arpp/Ankrd2 can interact with nuclear proteins, such as premyelocytic leukemia protein (PML), p53 and YB- 1 in vitro. In this study, to determine whether nuclear accumulation of Arpp/Ankrd2 actually occurs, we performed an immunohistochemical investigation of gastrocnemius muscles that had been injured by injection of cardiotoxin or contact with dry ice. We found that Arpp/Ankrd2 accumulated in the nuclei of myofibers located adjacent to severely damaged myofibers after muscle injury. Double-labeled immunohistochemistry revealed that Arpp/Ankrd2 accumulated in the nuclei of sarcomere-damaged myofibers. Furthermore, we found that Arpp/Ankrd2 tended to be localized in euchromatin where genes are transcriptionally activated. Based on these findings, we suggest that Arpp/Ankrd2 may translocate from the I-band to the nucleus in response to muscle damage and may participate in the regulation of gene expression.

  • Research Products

    (8 results)

All 2008 2007

All Journal Article (6 results) (of which Peer Reviewed: 3 results) Presentation (2 results)

  • [Journal Article] Arpp/Ankrd2,a member of the Muscle Ankyrin Repeat Proteins(MARPs),translocates from the I-band to the nucleus after muscle injury2008

    • Author(s)
      Tsukamoto Y, Hijiya N, Yano S, Yokoyama S, Nakada C, Uchida T, Matsuura K, Moriyama M.
    • Journal Title

      Histochemistry and Cell Biology 129

      Pages: 55-64

    • Description
      「研究成果報告書概要(和文)」より
    • Peer Reviewed
  • [Journal Article] Titled Arpp/Ankrd2, a member of the Muscle Ankyrin Repeat Proteins(MARPs), translocates from the Pband to the nucleus after muscle injury2008

    • Author(s)
      Y., Tsukamoto, N., Hijiya, S., Yano, S., Yokoyama, C., Nakada, T., Uchida, K., Matsuura, M., Morivama
    • Journal Title

      Histochemistry and Cell Biology vol.129

      Pages: 55-64

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Upregulated expressipn of cardiac ankyrin-repeated protein in renal podocytes is associated with proteinuria severity in lupus nephritis2007

    • Author(s)
      Matsuura K, Uesugi N, Hijiya N, Uchida T, Moriyama M
    • Journal Title

      Human Pathology 38

      Pages: 410-419

    • Description
      「研究成果報告書概要(和文)」より
    • Peer Reviewed
  • [Journal Article] High-resolution analysis of DNA copy number alterations and the gene expression in renal clear cell carcinoma2007

    • Author(s)
      Yoshimoto T, Matsuura K, Karnan S, Tagawa H, Nakada C, Tanigawa M, Tsukamoto Y, Uchida T, Kashima K, Akizuki S, Takeuchi I, Sato F, Mimata H, Seto M, Moriyama M.
    • Journal Title

      Journal of Pathology 213

      Pages: 392-401

    • Description
      「研究成果報告書概要(和文)」より
    • Peer Reviewed
  • [Journal Article] Title : Upregulated egression of cardiac ankyriirrepeated protein in renal podocytes is associated with proteinuria severity in lupus nephritis2007

    • Author(s)
      K., Matsuura, N., Uesugi, N., Hijiya, T., Uchida, M., Morivama
    • Journal Title

      Human Pathology vol.38

      Pages: 410-419

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Title : High-resolution analysis of DNA copy number alterations and the gene expression in renal clear cell carcinoma2007

    • Author(s)
      T., Yosbimoto, K., Matsuura, S., Karnan, H., Tagawa, C., Nakada, M., Tanigawa, Y., Tsukamoto, T., Uchida, K., Kashima, S., Akizuki, I., Takeuchi, F., Sato, H., Mimata, M., Seto, M., Morivama
    • Journal Title

      Journal of Pathology vol.213

      Pages: 392-401

    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] Title : High-resolution analysis of DNA copy number alterations and the gene expression in renal clear cell carcinoma2008

    • Author(s)
      Keiko, Matsuura, Taichiro, Yoshimoto, Sivasundaram, Karnan, Hiroyuki, Tagawa, Chisato, Nakada, Masato, Tanigawa, Yoshiyuki, Tsukamoto, Tomohisa, Uchida, IchiroTakeuchi, Fuminori, Sato, Hiromitsu, Mimata, Masao, Seto, Masatsugu, Moriyama
    • Organizer
      The 67th Annual Meeting of the Japanese Cancer As sociation
    • Place of Presentation
      Nagoya Congress Center
    • Year and Date
      20081028-30
    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] High-resolution analysis of DNA copy number alterations and the gene expression in renal clear cell carcinoma2007

    • Author(s)
      Keiko Matsuura, Taichiro Yoshimoto, Sivasundaram Kaman, Hiroyuki Tagawa, Chisato Nakada, Masato Tanigawa, Yoshiyuki Tsukamoto, Tomohisa Uchida, Ichiro Takeuchi, Fuminori Sato, Hiromitsu Mimata, Masao Seto, Masatsugu Moriyama
    • Organizer
      第66回日本癌学会学術総会
    • Place of Presentation
      横浜市(パシフィコ横浜)
    • Year and Date
      2007-10-04
    • Description
      「研究成果報告書概要(和文)」より

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Published: 2010-02-04  

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