Development of DNA polymerase ι-deficient mice and characterization of their susceptibility to lung tumor development

2008 Fiscal Year Final Research Report

• Project/Area Number: 18590384
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Experimental pathology
• Research Institution: Kochi University
• Principal Investigator: LEE Gang-hong Kochi University, 教育研究部医療学系, 教授 (10261405)
• Project Period (FY): 2006 – 2008
• Keywords: 腫瘍 / 発がん感受性 / 量的形質 / 連鎖解析

Research Abstract

DNA ポリメラーゼイオタは、我々が発見した肺発がん抵抗性遺伝子座の有力な候補遺伝子である。この遺伝子の欠損が肺発がんに及ぼす影響を直接的に調べるため、DNAポリメラーゼイオタ欠損マウスの樹立を遺伝子工学技術によって試み、成功した。しかし、樹立の過程は様々な要因により当初計画よりも遅れを生じたため、3 年間の研究期間で肺発がん感受性実験の終了にまで至ることができなかった。2009 年度以降も外部資金獲得に努め、研究を継続する。

Research Products

• [Journal Article] Ureteral small cell carcinoma (2009)
  • Author(s): Kuroda N, Katto K, TamuraM, Shiotsu T, Inoue K, Ohara M, Mizuno K, Lee G-H.
  • Journal Title: Med Mol Morphol 42 Pages: 55-57
  • Peer Reviewed
• [Journal Article] Inhibition of neointimal hyperplasia development by MCI-186 is correlated with downregulation of nuclear factor-kappaB pathway (2008)
  • Author(s): Radhakrishnan G, Suzuki R, Maeda H, Yamamoto M, Hirose N, Gopalrao RK, Lee G-H, Hayashi Y, Rao P, Sasaguri S.
  • Journal Title: Circ J. 72 Pages: 800-806
  • Peer Reviewed
• [Journal Article] Carcinoid tumor of the renal pelvis : consideration on the histogenesis (2008)
  • Author(s): Kuroda N, Katto K, TamuraM, Shiotsu T, Hes O, Michal M, Nagashima Y, Ohara M, Hirouchi T, Mizuno K, Hayashi Y, Lee G-H.
  • Journal Title: Pathol Int. 58 Pages: 51-54
  • Peer Reviewed
• [Journal Article] Histochemical demonstration of aluminum and iron deposition in pulmonary bony tissues in three cases of diffuse pulmonary ossification (2008)
  • Author(s): Ohtsuki Y, Yamanaka A, Ohyama H, Yamada E, Terada N, Fujita J, Lee G-H, Furihata M.
  • Journal Title: Histol Histopathol 23 Pages: 137-141
  • Peer Reviewed
• [Journal Article] Radhakrishnan G, Rao P, Sasaguri S. The local injection of peritoneal macrophages induces neovascularization in rat ischemic hind limb muscles (2008)
  • Author(s): Hirose N, Maeda H, Yamamoto M, Hayashi Y, Lee G-H, Chen L.
  • Journal Title: Cell Transplant 17 Pages: 211-222
  • Peer Reviewed
• [Journal Article] Possible identification of third stromal component in extraadrenal paraganglioma : myofibroblast in fibrous band and capsule (2008)
  • Author(s): Kuroda N, Tamura M, Ohara M, Hirouchi T, Mizuno K, Miyazaki E, Hayashi Y, Lee G-H.
  • Journal Title: Med Mol Morphol 41 Pages: 59-61
  • Peer Reviewed
• [Journal Article] Furihata M. Multiple minute nests of incidentally detected paraganglionic cells associated with urothelial carcinoma of the urinary bladder in a 73 year-old woman (2008)
  • Author(s): Ohtsuki Y, Ochi K, Okada Y, Lee G-H.
  • Journal Title: Med Mol Morphol 41 Pages: 62-65
  • Peer Reviewed
• [Journal Article] Significance of D2-40 expression in the diagnosis of gastrointestinal stromal tumor (2008)
  • Author(s): Kuroda N, Tanida N, Oonishi K, Inoue K, Ohara M, Mizuno K, Taguchi T, Hayashi Y, Hanazaki K, Lee G-H.
  • Journal Title: Med Mol Morphol 41 Pages: 109-112
  • Peer Reviewed
• [Journal Article] Basal-like carcinoma of the breast : further evidence of the possibility that most metaplastic carcinomas may be actually basal-like carcinomas (2008)
  • Author(s): Kuroda N, Fujishima N, Inoue K, Ohara M, Hirouchi T, Mizuno K, Hayashi Y, Lee G-H.
  • Journal Title: Med Mol Morphol 41 Pages: 117-120
  • Peer Reviewed
• [Journal Article] Furihata M. Micropapillary component of urothelial carcinoma detected in transurethral resection of bladder tumor (TUR-BT) tissues : a case report (2008)
  • Author(s): Ohtsuki Y, Ochi K, Okada Y, Kato M, Lee G-H.
  • Journal Title: Med Mol Morphol 41 Pages: 113-116
  • Peer Reviewed
• [Journal Article] Furihata M. Immunohistochemical localisation of surfactant proteins A and D, and KL-6 in pulmonary alveolar proteinosis (2008)
  • Author(s): Ohtsuki Y, Kobayashi M, Yoshida S, Kishimoto N, Kubo K, Yokoyama A, Lee G-H.
  • Journal Title: Pathology 40 Pages: 536-539
  • Peer Reviewed
• [Journal Article] Sarcomatoid acquired cystic disease-associated renal cell carcinoma (2008)
  • Author(s): Kuroda N, Tamura M, Taguchi T, Tominaga A, Hes O, Michal M, Ohara M, Hirouchi T, Mizuno K, Hayashi Y, Shuin T, Lee G-H.
  • Journal Title: Histol Histopathol 23 Pages: 1327-1331
  • Peer Reviewed
• [Journal Article] A rare case of coexistence of pulmonary adenocarcinoma with Langerhans' cell histiocytosis (2008)
  • Author(s): Ohtsuki Y, Uomoto M, Hachisuka Y, Kato M, Iguchi M, Lee G-H, Furihata M.
  • Journal Title: Med Mol Morphol 41 Pages: 175-178
  • Peer Reviewed
• [Journal Article] Elastofibroma : a histochemical, immunohistochemical, and ultrastructural study of two patients (2008)
  • Author(s): Kuroda N, Hamaguchi N, Ohara M, Hirouchi T, Mizuno K, Hayashi Y, Lee G-H.
  • Journal Title: Med Mol Morphol 41 Pages: 179-182
  • Peer Reviewed
• [Journal Article] Mucinous tubular and spindle cell carcinoma with Fuhrman nuclear grade 3 : a histological, immunohistochemical, ultrastructural and FISH study (2008)
  • Author(s): Kuroda N, Hes O, Michal M, Nemcova J, Gal V, Yamaguchi T, Kawada T, Imamura Y, Hayashi Y, Lee G-H.
  • Journal Title: Histol Histopathol 23 Pages: 1517-1523
  • Peer Reviewed
• [Journal Article] Expression of fascin-1, an actin-bundling protein, in migrating hepatoblasts during rat liver development (2008)
  • Author(s): Hayashi Y, Toda K, Saibara T, Okamoto S, Osanai M, Enzan H, Lee G-H.
  • Journal Title: Cell Tissue Res. 334 Pages: 219-226
  • Peer Reviewed
• [Journal Article] Ultrastructural study of the morphogenesis of human herpesvirus 6 type B in human T-lymphotropic virus type I-producing lymphoid cells (2008)
  • Author(s): Ohtsuki Y, Daibata M, Bandobashi K, Lee G-H, Furihata M, Yokoyama A, Miyoshi I.
  • Journal Title: Med Mol Morphol 41 Pages: 204-210
  • Peer Reviewed
• [Journal Article] Chromophobe renal cell carcinoma : useful diagnostic application of imprint cytology and fluorescence in situ hybridization of chromosomes 10 and 21 in two cases of typical and eosinophilic variants (2008)
  • Author(s): Kuroda N, Katto K, Yamaguchi T, Kawada T, Imamura Y, Hes O, Michal M, Shuin T, Lee G-H.
  • Journal Title: Med Mol Morphol 41 Pages: 227-232
  • Peer Reviewed
• [Journal Article] Coexistent adenomyoepithelioma and invasive ductal carcinoma of the breast : presentation as separate tumors (2008)
  • Author(s): Kuroda N, Fujishima N, Ohara M, Hirouchi T, Mizuno K, Hayashi Y, Lee G-H.
  • Journal Title: Med Mol Morphol 41 Pages: 238-242
  • Peer Reviewed
• [Journal Article] The Kras2 oncogene and mouse lung carcinogenesis (2008)
  • Author(s): Lee G-H.
  • Journal Title: Med Mol Morphol 41 Pages: 199-203
  • Peer Reviewed
• [Journal Article] Mixed testicular germ cell tumor in an adult with cryptorchidism and Down's syndrome (2007)
  • Author(s): Kuroda N, Amano S, Shiotsu T, Tamura M, Hes O, Michal M, Lee G-H.
  • Journal Title: APMIS 115 Pages: 1292-1295
  • Peer Reviewed
• [Journal Article] H. An Asian variant of intravascular lymphoma : unique clinical and pathological manifestation in the gallbladder (2007)
  • Author(s): Kuroda N, Mizobuchi M, Shimamura Y, Taniguchi Y, Daibata M, Miyoshi I, Ichimura T, Beppu H, Ohara M, Hirouchi T, Mizuno K, Lee G-H.
  • Journal Title: APMIS 115 Pages: 371-375
  • Peer Reviewed
• [Journal Article] Renal leiomyoma : an immunohistochemical, ultrastructural and comparative genomic hybridization study (2007)
  • Author(s): Kuroda N, Inoue Y, Taguchi T, Tominaga A, Hes O, Michal M, Hayashi Y, Hiroi M, Shuin T, Lee G-H.
  • Journal Title: Histol Histopathol 22 Pages: 883-888
  • Peer Reviewed
• [Journal Article] Invasive ductal carcinoma of the breast with signet-ring cell and mucinous carcinoma components : diagnostic utility of immunocytochemistry of signet-ring cells in aspiration cytology materials (2007)
  • Author(s): Kuroda N, Fujishima N, Ohara M, Hirouchi T, Mizuno K, Lee G-H.
  • Journal Title: Diagn Cytopathol 35 Pages: 171-173
  • Peer Reviewed
• [Journal Article] Bilirubin oxidation provoked by nitric oxide radicals predicts the progression of acute cardiac allograft rejection (2007)
  • Author(s): Yamamoto M, Maeda H, Hirose N, Radhakrishnan G, Katare RG, Hayashi Y, Rao P, Lee G-H, Yamaguchi T, Sasaguri S.
  • Journal Title: Am J Transplant 7 Pages: 1897-1906
  • Peer Reviewed
• [Journal Article] Invasive micropapillary carcinoma of the colon : an immunohistochemical study (2007)
  • Author(s): Kuroda N, Oonishi K, Ohara M, Hirouchi T, Mizuno K, Hayashi Y, Lee G-H.
  • Journal Title: Med Mol Morphol 40 Pages: 226-230
  • Peer Reviewed
• [Journal Article] Anaplastic carcinoma of the pancreas with rhabdoid features and hyaline globule-like structures (2007)
  • Author(s): Kuroda N, Iwamura S, Fujishima N, Ohara M, Hirouchi T, Mizuno K, Hayashi Y, Lee G-H.
  • Journal Title: Med Mol Morphol 40 Pages: 168-171
  • Peer Reviewed
• [Journal Article] Large cell neuroendocrine carcinoma of the lung with calcification and expression of osteopontin (2007)
  • Author(s): Kuroda N, Hamaguchi N, Ohara M, Hirouchi T, Mizuno K, Lee G-H.
  • Journal Title: Med Mol Morphol 40 Pages: 218-220
  • Peer Reviewed
• [Journal Article] Bridging necrosis and reticulin bridging fibrosis induced by intrahepatic involvement of acute biphenotypic leukemia (2006)
  • Author(s): Kuroda N, Mizobuchi M, Shimamura Y, Daibata M, Miyoshi I, Ohara M, Hirouchi T, Mizuno K, Lee G-H.
  • Journal Title: APMIS 114 Pages: 908-911
  • Peer Reviewed
• [Journal Article] Askin tumor with metastasis to the scalp : a histochemical, immunohistochemical and ultrastructural study (2006)
  • Author(s): Kuroda N, Takahashi T, Moriki T, Okanoue Y, Mizobuchi H, Miyazaki E, Hayashi Y, Lee G-H.
  • Journal Title: Med Mol Morphol 39 Pages: 221-225
  • Peer Reviewed
• [Presentation] マウスを利用した肺発がん感受性の研究 -マウスからヒトへ、ヒトからマウスへ- (2008)
  • Author(s): 李康弘、林芳弘
  • Organizer: 第97回日本病理学会総会
  • Place of Presentation: 金沢
  • Year and Date: 2008-05-16
• [Presentation] Genetic basis for the lung tumor resistance of BALB/cByJ mice (2007)
  • Author(s): Lee G-H
  • Organizer: First JCA-AACR Special Joint Conference
  • Place of Presentation: Nagoya, Japan
  • Year and Date: 20070312-14