2007 Fiscal Year Final Research Report Summary
Basic fibroblast growth factor induces downregulation of alpha smooth muscle actinin dermal myofibroblasts in vivo and in vitro, leading to inhibition of fibrosis
Project/Area Number |
18590385
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
Experimental pathology
|
Research Institution | Toho University |
Principal Investigator |
AKASAKA Yoshikiyo Toho University, Faculty of Medicine, Associate Professor (60202511)
|
Co-Investigator(Kenkyū-buntansha) |
ISHIKAWA Yukio Toho University, Faculty of Medicine, Associate Professor (30276894)
ONO Ichiro Sappro Medical University, School of Medicine, Associate Professor (20125298)
|
Project Period (FY) |
2006 – 2007
|
Keywords | Wound healine / Mvofibroblast / Apoptosis / hFGF / TGF-β1 / α-SMA |
Research Abstract |
To examine the effect of bFGF on inhibition of a-SMA expression in dermal fibroblasts, we have established two dermal myofibroblastic lines positive for a-SMA RMFL cell lines) and one fibroblastic line negative for a-SMA (RF cell line) as a model of fibroblast differentiation. In contrast to the increased expression of a-SMA in the RMF and RMFL cell lines with or without TGF-β1 treatment, bFGF induced a decrease of a-SMA expression in the myofibroblastic lines and the reduced expression patterns of a-SMA were different between them, as demonstrated by Western blot and RT-PCR analyses. Along with the inhibition of a-SMA expression in the RMF and RMFL cell lines by bFGF, different activated expression of ERK1/2 were also detected in them, suggesting the involvement of ERK1/2 activation in downregulation of a-SMA expression in the myofibroblastic lines. Furthermore, in vivo study demonstrated that bFGF administration decreased the area positive for a-SMA expression in the open wounds in the later phase of healing. These results together indicate that bFGF is a potent stimulator of downregulation of α-SMA expression in dermal myofibroblasts, suggesting the possible role of bFGF in the modulation toward fibroblasts from myofibroblasts in vivo.
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[Journal Article] bFGF in artificial dermis promotes apoptosis and inhibits expression of a-smooth muscle actin, leading to reduction of wound contraction2007
Author(s)
Akasaka, Y., Ono, I., Tominaga, A., Ishikawa, Y., Ito, K., Suzuki, T., Imaizumi, R., Ishiguro, S., Jimbow, K., Ishii, T
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Journal Title
Wound Repair Regen 15
Pages: 378-389
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Basic fibroblast growth factor reduces scar formation in acute incisional wounds2007
Author(s)
Ono, I., Akasaka, Y., Kikuchi, R., Sakemoto, A., Kamiya, T., Yamashita, T., Jimbow, K
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Journal Title
Wound Repair Regen 15
Pages: 617-623
Description
「研究成果報告書概要(欧文)」より
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