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2007 Fiscal Year Final Research Report Summary

Genetic regulation by the environmental signals in Clostridium perfringens

Research Project

Project/Area Number 18590415
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Bacteriology (including Mycology)
Research InstitutionKanazawa University

Principal Investigator

SHIMIZU Tohru  Kanazawa University, Graduate School of Medical Science, Professor (80235655)

Project Period (FY) 2006 – 2007
KeywordsClostridium perfringens / gas gangrene / microarray / host factor / virulence regulation
Research Abstract

Gram-positive spore-forming bacteria, Clostridium perfringens is a causative agent for gas gangrene (clostridial myonecrosis) and food poisoning in humans. C. perfringens produces numerous toxins and enzymes, which are responsible for the pathogenicity through synergestic actions of toxins and/or enzymes on their specific targets in the tissue. Many genes for toxins and enzymes are regulated by the two-component VirR/VIrS system (VirS and VirR are sensor kinase and response regulator, respectively), and many other secondary regulators including regulatory RNA molecules (ex. VR-RNA and virX) might be also involved in the genetic regulation of the virulence genes in C. perfringens
According to the genomic sequence of C. perfringens strain13, we have designed DNA microarrays, which were used to compare the gene expression profiles between wild-type and various mutants of specific regulatory genes. Total of >100 microarray data was obtained and they were analyzed using various bio-informatic methods. Numerous operons and regulons were identified to contain genes that were always co-regulated in the same manner in every experiment. One of the regulons was the VirR/VirS regulon including 37 genes directly regulated by the VirR/VirS system and approximately 100 genes that are regulated by the secondary regulator (VR-RNA), indicating that the VirR/VirS system regulates many genes involved not only in virulence (ex. phospholipase C, perfringolysin O, collagenase) but also in metabolism (amino acid synthesis, transporters) and energy production (sugar utilization).

  • Research Products

    (10 results)

All 2008 2007 2006

All Journal Article (7 results) (of which Peer Reviewed: 3 results) Presentation (3 results)

  • [Journal Article] Carbon catabolite repression of type IV pilus-dependent gliding motility in theanaerobic pathogen Clostridium perfringens.2008

    • Author(s)
      Mendez, M., H. Huang, K. Ohtani, R. Grau. T. Shimizu. M. R. Sarker.
    • Journal Title

      J. Bacteriol. 190

      Pages: 48-60

    • Description
      「研究成果報告書概要(和文)」より
    • Peer Reviewed
  • [Journal Article] Characterization of genes regulated directly by the VirR/VirS system in Clostridium perfringens2008

    • Author(s)
      Okumura, K., K. Ohtani, H. Hayashi, T. Shimizu.
    • Journal Title

      J. Bacteriol. 190

      Pages: 7719-7727

    • Description
      「研究成果報告書概要(和文)」より
    • Peer Reviewed
  • [Journal Article] Carbon catabolite repression of type IV pilus-dependent gliding motility in the anaerobic pathogen Clostridium perfringens.2008

    • Author(s)
      Mendez, M., I. H. Huang, K. Ohtani, R. Grau, T. Shimizu, M. R. Sarker.
    • Journal Title

      J. Bacteriol. 190

      Pages: 48-60

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Characterization of genes regulated directly by the VirR/VirS system in Clostridium perfringens.2008

    • Author(s)
      Okumura, K., K. Ohtani, H. Hayashi, T. Shimizu.
    • Journal Title

      J. Bacteriol. 190

      Pages: 7719-7727

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Complete genome sequence of Finegoldia magna, an anaerobic opportunistic pathogen.2008

    • Author(s)
      Goto, T., A. Yamashita, H. Hirakawa, M. Matsutani, K. Todo, K. Ohshima, H. Toh, K. Miyamoto, S. Kuhara, M. Hattori, T. Shimizu, S. Akimoto.
    • Journal Title

      DNA Res. 15

      Pages: 39-47

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Detection of a group II intron without open reading frame in the alphatoxin gene of Clostridium perfringens isolated from a broiler chicken2007

    • Author(s)
      Ma, M., Ohtani, K., Shimizu, T., Misawa, N.
    • Journal Title

      J. Bacteriol. 60

      Pages: 1633-1640

    • Description
      「研究成果報告書概要(和文)」より
    • Peer Reviewed
  • [Journal Article] Detection of a group II intron without an open reading frame in the alpha-toxin gene of Clostridium perfringens isolated from a broiler chicken.2007

    • Author(s)
      Ma, M., K. Ohtani, T. Shimizu, N. Misawa.
    • Journal Title

      J. Bacteriol. 189

      Pages: 1633-1640

    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] Effect of bacterial and host factors on gene expression in Clostridium perfringens.2007

    • Author(s)
      K. Ohtani, A. Isu, J. Ohkubo, T. Shimizu.
    • Organizer
      107^<th> ASM General Meeting
    • Place of Presentation
      Tronto, Canada
    • Year and Date
      20070521-20070525
    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] Effect of host factors on gene expression in Clostridium perfringens.2007

    • Author(s)
      K. Ohtani, A. Isu, J. Ohkubo, T. Shimizu.
    • Organizer
      107^<th> ASM General Meeting
    • Place of Presentation
      トロント、カナダ
    • Year and Date
      2007-05-24
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] Biological signaling to gene expression in Clostridium perfringens.2006

    • Author(s)
      K. Ohtani, K. Tanaka, K. Hasatani, K. Miwa, A. Isu, T. Shimizu.
    • Organizer
      5^<th> International Meeting on the Molecular Biology and Pahogenesis of the Clostridia.
    • Place of Presentation
      Nottingham, UK.
    • Year and Date
      20060621-20060625
    • Description
      「研究成果報告書概要(欧文)」より

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Published: 2010-06-09  

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