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2007 Fiscal Year Final Research Report Summary

Analysis of Maturation pathway protein toxin of Enteric Bacteria and Studies of Prevention of Diarrhea

Research Project

Project/Area Number 18590423
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Bacteriology (including Mycology)
Research InstitutionOkayama University

Principal Investigator

OKAMOTO Keinosuke  Okayama University, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Professor (70131183)

Co-Investigator(Kenkyū-buntansha) YAMANAKA Hiroyasu  Hiroshima International University, Faculty of Pharmaceutical Sciences, Professor (30202386)
NAKAO Hiroshi  Okayama University, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Associate Professor (20237217)
TAKAHASHI Eizo  Okayama University, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Assistant Professor (70379733)
Project Period (FY) 2006 – 2007
KeywordsEnteric infection / Bacterial toxin / Maturation pathway / Enterotoxin / Protease / Hemolvsin / Escherichia coli / Aeromonas
Research Abstract

The studies on maturation pathway of enterotoxin and protease of Aeromonas and heat-stable enterotoxin of enterotoxigenic Escherichia coil were performed in this study. The diarrheagenic toxin of Aeromonas has rapacity to lyse erythrocytes, therefore, another name the toxin is hemolysin. The culture filtrates of A. sobria and A. hydrophila exhibit hemolytic activity, because the hemolysin is produced into the solution. However, the filtrate of A. caviae does not exhibit However, A. caviae caused diarrhea in humans. We examined the production of diarrheagenic toxin of A. caviae. It has been shown that A. caviae synthesis the hemolysin and release it into outside of the cells, however, the hemolysin is broken by the proteases produced by A. caviae. Therefore, the culture filtrate of A. caviae does not exhibit hemolytic activity. In intestinal lumen and agar medium, these hemolysins produced by A. caviae can bind the appropriate cells and express the activity before these hemolysins are broken by proteases. Another finding of this research project is that production of serineprotease and metallo protease of A. sobria is inhibited by salt (sodium chloride) in the medium. hi the medium containing sodium chloride at the ratio of more than 2%, the production of these proteases is significantly repressed. The studies on the mechanism of the repression showed that the repressions of production of serineprotease and metallo pro ease are derived from inhibitions of maturation pathway and of transcriptional stage of each toxin, respectively. In the study about Escherichia coil, the structure of TolC which is involved in the translocation of ST(heat-stable enterotoxin) across the outer membrane was examined. To examined, we constructed the chimeric TolC which is consisted of E. coli TolC and Vibrio parahaemolyticus TolC. The result shows that the region covering 198th -214th of E. coli TolC plays an important role in the transport of ST.

  • Research Products

    (23 results)

All 2008 2007 2006

All Journal Article (10 results) (of which Peer Reviewed: 5 results) Presentation (12 results) Patent(Industrial Property Rights) (1 results) (of which Overseas: 1 results)

  • [Journal Article] Fluid Secretion caused by Aerolysin-like Hemolysin of Aeromonas sobria in the Intestines Is Due to Stimulation of Production of Prostaglandin E2 via Cyclooxygenase-2 by the Intestinal Cells2008

    • Author(s)
      Yoshio Fujii
    • Journal Title

      Infection and Immunity 76

      Pages: 1076-1082

    • Description
      「研究成果報告書概要(和文)」より
    • Peer Reviewed
  • [Journal Article] Fluid Secretion caused by Aerolysin-like Hemolysin of Aeromonas sobria in the Intestines Is Due to Stimulation of Production of Prostaglandin E2 via Cyclooxygenase-2 by the Intestinal Cells Infection and Immunity2008

    • Author(s)
      Yoshio, Fujii, Ken, Tsurumi, Masaaki, Sato, Eizo, Takahashi, Keinosuke, Okamoto
    • Journal Title

      Infection and Immunity 76(3)

      Pages: 1076-1082

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Studies on the region involved in the transport activity of Eshcrichia coli TolC by chemeric protein analysis2007

    • Author(s)
      Hiroyasu Yamanaka
    • Journal Title

      Microbial Pathogenesis 42

      Pages: 184-192

    • Description
      「研究成果報告書概要(和文)」より
    • Peer Reviewed
  • [Journal Article] Salt in Surroundings Influences the Production of Serine Protease into Milieu by Aeromonas sobria2007

    • Author(s)
      Rasel Khan
    • Journal Title

      Microbiology and Immunology, 51

      Pages: 963-976

    • Description
      「研究成果報告書概要(和文)」より
    • Peer Reviewed
  • [Journal Article] Studies on the region involved in the transport activity of Eshcrichia coli TolC by chemeric protein analysis2007

    • Author(s)
      Hiroyasu, Yamanaka, Satoshi, Tadokoro, Masaya, Miyano, Eizo, Takahashi, Hidetomo, Kobayashi, Keinosuke, Okamoto
    • Journal Title

      Microbial Pathogenesis 42

      Pages: 184-192

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Salt in Surroundings Influences the Production of Serine Protease into Milieu by Aeromonas sobria2007

    • Author(s)
      Rasel, Khan, Eizo, Takahashi, Thandavarayan, Ramamurthy, Yoshifumi, Takeda, Keinosuke, Okamoto
    • Journal Title

      Microbiology and Immunology 51(10)

      Pages: 963-976

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Cleavage specificity of the serine protease of Aeromonas sobria, a member of the kexin family of subtilases2006

    • Author(s)
      Hidetomo Kobayashi
    • Journal Title

      FEMS Microbiology Letters 256

      Pages: 165-170

    • Description
      「研究成果報告書概要(和文)」より
    • Peer Reviewed
  • [Journal Article] Induction of vascular leakage and blood pressure lowering through kinin release by a seine protease from Aeromonas sobria2006

    • Author(s)
      Takahisa Imamura
    • Journal Title

      Journal of Immunology 177

      Pages: 8723-8729

    • Description
      「研究成果報告書概要(和文)」より
    • Peer Reviewed
  • [Journal Article] Cleavage specificity of the serine protease of Aeromonas sobria, a member of the kexin family of subtilases2006

    • Author(s)
      Hidetomo, Kobayashi, Eizo, Takahashi, Keiji, Oguma, Yoshio, Fujii, Hiroyasu, Yamanaka, Tomoe, Negishi, Sakae, Arimoto-Kobayashi, Takao, Tsuji, Keinosuke, Okamoto
    • Journal Title

      FEMS Microbiology Letters 256(1)

      Pages: 165-170

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Induction of vascular leakage and blood pressure lowering through kinin release by a seine protease from Aeromonas sobria2006

    • Author(s)
      Takahisa, Imamura, Hidetomo, Kobayashi, Rasel, Khan, Hidetoshi, Nitta, Keinosuke, Okamoto
    • Journal Title

      Journal of Immunology 177(12)

      Pages: 8723-8729

    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] Crystal Structure of Aeromonas sobria Serine Protease, Protptype Kexin/Furin Protease: The Extra Occluding Domain Regulates Protein Substrate Specificity2007

    • Author(s)
      Hideaki Tsuge
    • Organizer
      5th General Meeting of the International Proteolysis Society (IPS2007)
    • Place of Presentation
      ギリシャ,パトラス
    • Year and Date
      20071020-24
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] Crystal Structure of Aeromonas sobria Serine Protease, Protptype Kexin/Furin Protease : The Extra Occluding Domain Regulates Protein Substrate Specificity2007

    • Author(s)
      Hideaki, Tsuge
    • Organizer
      5th General Meeting of the International Proteolysis Society (11)52007)
    • Place of Presentation
      Patras, Greece
    • Year and Date
      20071020-24
    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] Production of metalloprotease by Aeromonas sobria is disturbed by the salt in surrounding2007

    • Author(s)
      Eizo Takahashi
    • Organizer
      Joint Conference on Cholera and Other Bacterial Enteric Infections
    • Place of Presentation
      米国、テキサス州
    • Year and Date
      2007-12-06
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] Production of metalloprotease by Aeromonas sobria is disturbed by the salt in surrounding2007

    • Author(s)
      Eizo, Takahashi
    • Organizer
      Joint Conference on Cholera and Other Bacterial Enteric Infections
    • Place of Presentation
      Austin, Texas, USA
    • Year and Date
      2007-12-06
    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] Influence of salt in surroundings on the production of proteases into milieu by A eromonas sobria2007

    • Author(s)
      Keinosuke Okamoto
    • Organizer
      US-Japan Cholera Meeting, Japanese Panel
    • Place of Presentation
      大阪
    • Year and Date
      2007-08-01
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] Influence of salt in surroundings on the production of proteases into milieu by Aeromonas sobria2007

    • Author(s)
      Keinosuke, Okamoto
    • Organizer
      US-Japan Cholera Meeting, Japanese Panel
    • Place of Presentation
      Osaka, Japan
    • Year and Date
      2007-08-01
    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] Salt in Surroundings Regulates Production of Proteases of Aeromonas sobria2006

    • Author(s)
      Eizo Takahashi,
    • Organizer
      Joint Conference on Cholera and Other Bacterial Enteric Infections
    • Place of Presentation
      岐阜市
    • Year and Date
      20061105-07
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] Salt in Surroundings Regulates Production of Proteases of Aeromonas sobria2006

    • Author(s)
      Eizo, Takahashi
    • Organizer
      Joint Conference on Cholera and Other Bacterial Enteric Infections
    • Place of Presentation
      Gifu, Japan
    • Year and Date
      20061105-07
    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] アエロモナスの菌体外毒素産生と環境2006

    • Author(s)
      岡本敬の介
    • Organizer
      第59回日本細菌学会中国・四国支部総会
    • Place of Presentation
      山口県宇部市
    • Year and Date
      20061017-18
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] Exotoxins of Aeromonas and Surrounding Conditions2006

    • Author(s)
      Keinosuke, Okamoto
    • Organizer
      59th Chugoku-Shikoku Meetings of Japanese Bacteriological Society
    • Place of Presentation
      Ube, Yamaguchi, Japan
    • Year and Date
      20061017-18
    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] Analysis of maturation pathway of proteases of Aeromonas and effect of salt insurroundings on the maturation2006

    • Author(s)
      Keinosuk Okamoto
    • Organizer
      US-Japan Cholera Meeting, Japanese Panel
    • Place of Presentation
      大阪
    • Year and Date
      2006-08-18
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] Analysis of maturation pathway of proteases of Aeromonas and effect of salt in surroundings on the maturation2006

    • Author(s)
      Keinosuk, Okamoto
    • Organizer
      US-Japan Cholera Meeting, Japanese Panel
    • Place of Presentation
      Osaka, Japan
    • Year and Date
      2006-08-18
    • Description
      「研究成果報告書概要(欧文)」より
  • [Patent(Industrial Property Rights)] スルホンアミド基を有する抗菌剤2006

    • Inventor(s)
      加来田博貴、他8名
    • Industrial Property Rights Holder
      同左
    • Industrial Property Number
      2007-073738、PCT/JP2008/55140
    • Filing Date
      2006-11-08
    • Description
      「研究成果報告書概要(和文)」より
    • Overseas

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Published: 2010-02-04  

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