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2007 Fiscal Year Final Research Report Summary

Analysis of Uropathogenic-specific protein and development of the mucosal vaccine by using of USP

Research Project

Project/Area Number 18590433
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Bacteriology (including Mycology)
Research InstitutionObihiro University of Agriculture and Veterinary Medicine (2007)
Osaka Prefecture University (2006)

Principal Investigator

KURAZONO Hisao  Obihiro University of Agriculture and Veterinary Medicine, Dept. of Veterinary Bacteriology, Professor (90186487)

Co-Investigator(Kenkyū-buntansha) HOSHI Hidenobu  Osaka Prefecture University, Dept. of Veterinary Medicine, Research Associate (30301188)
YAMAMOTO Shingo  Hyogo Medical University, 医学部, Associate Professor (80322741)
MAKINO Souichi  Obihiro University, National Research Center for Protozoan Diseases, Director (30181621)
Project Period (FY) 2006 – 2007
KeywordsUrinary Tract Infection / Escherichia coli / Protein Toxin / USP / Mucosal Vaccine
Research Abstract

Using crystal violet binding assay, we examined the ability of biofilm formation in 194, 76, 107 isolates from uncomplicated acute cystitis, pyelonephritis and prostatitis, respectively. The prostatitis isolates showed significantly higher OD values than cyctitis and pyelonephritis isolates. Similarly, strains of serotypes O4 and O22, which were frequently isolated from prostatitis, exhibited significantly higher OD values than other strains. Further, when the 21 prostatitis solates were examined for expression of curli fimbriae, 8 of 12 strains showing high OD value but only 3 of 9 showing low OD value expressed curli fimbriae. These results suggest an association between acute bacterial prostatitis and biofilm formation (kanamaru S. et al, Int. J. Urol. 2006, 28 (S1): S21-S25).
A total of 427 Escherichia coli isolates from uncomplicated UTI (194 cystitis, 76 pyelonephritis, and 107 prostatitis) and 50 fecal isolates were examined for the phylogenetic grouping and PAI-usp subtyping as … More well as the prevalence of virulence factors (VFs) and O serogroups. Both phylogenetic group B2 and usp-positive strains were equally predominant in cystitis, pyelonephritis and prostatitis. Furthermore, each PAI-usp subtype was shown to be closely associated with several VF genes as well as several common O serogroups of UPEC. In molecular epidemiological studies, PAI-usp subtyping will provide additional informative findings of E. coli strains belonging to phylogenetic group B2 (Kanamaru S. et al, Int. Antimicrob. Agent 2006, 13: 754-760).
The genetic and serological characteristics of Escherichia coli isolates from patients with uncomplicated cystitis (UC), complicated cystitis (CC), and complicated asymptomatic bacteriuria (CASB) were determined. Phylogenetic group B2 was predominant in all categories. The prevalences of 14 out of 18 virulence factors genes were similar among the three categories, while pap, iha, ompT, and PAI were more frequently seen in isolates associated with UC than CC or CASB (Takahashi A et al, J. Clin. Microb. 2006, 44: 4589-4592). Less

  • Research Products

    (21 results)

All 2008 2007 2006

All Journal Article (8 results) (of which Peer Reviewed: 4 results) Presentation (13 results)

  • [Journal Article] Molecular characterization of Helicobacter pylori VacA-induction of IL-8 in U937 cells reveals a prominent role for p38 MAP kinase in ATF-2, CREB and NF-kappaB activation.2008

    • Author(s)
      Hisatsune J, Nakayama M, Isomoto H, Kurazono H , et. al.
    • Journal Title

      J. Immunol. 180

      Pages: 5017-5027

    • Description
      「研究成果報告書概要(和文)」より
    • Peer Reviewed
  • [Journal Article] Molecular typing of Vibrio cholerae O1 isolates from Thailand by pulsed-field gel electrophoresis.2008

    • Author(s)
      Tapchaisri P, Na-Ubol M, Nair GB, Kurazono H , et. al.
    • Journal Title

      L.Health Popu. Nutr. (印刷中)

    • Description
      「研究成果報告書概要(和文)」より
    • Peer Reviewed
  • [Journal Article] Molecular characterization of Helicobacter pylori VacA-induction of IL-8 in U937 cells reveals a prominent role for p38MAP kinase in ATF-2, CREB and NF- κB activation.2008

    • Author(s)
      Hisatsune, J., Nakayama, M., Isomoto. H., Kurazono, H., Mukaida, N., Mukhopadhyay, A.K., Sap, J., Yamasaki, E., Yahiro, Y., Moss, J., and Hirayama, T.
    • Journal Title

      J. Immunol. 180

      Pages: 5017-5027

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Molecular typing of Vibrio cholerae O1 isolates from Thailand by pulsed-field gel electrophoresis.2008

    • Author(s)
      Tapchaisri, P., Na-Ubol, M., Tiyasuttipan, W., Chaiyaroj, S.C., Yamasaki, S., Wongsaroj, T., Hayashi, H., Nair, G.B., Chongsa-nguan, M., Kurazono, H., Chaicumpa, W.
    • Journal Title

      J. Health Popul. Nutr. (In press)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Helicobacter pylori VacA enhance PGE-2 production through induction of cyclooxygenase 2 expressionvia a p38 MAP kinase/ ATF-2 cascade in AZ-521 cells.2007

    • Author(s)
      Hisatsune J, Yamasaki E, Nakayama M, Shirasaka D, Kurazono H , et. al.
    • Journal Title

      Infect. Immun. 75

      Pages: 4472-448

    • Description
      「研究成果報告書概要(和文)」より
    • Peer Reviewed
  • [Journal Article] Virulence genes of clinicai Vibrio cholerae O1 isolates in Thailand and their ribotypes.2007

    • Author(s)
      Tapchaisti, P., Na-Ubol, M., Jaipaew, J., Srimanote, P., Chongsa-Nguan, M., Yamasaki, S., Hayashi, H., Kurazono, H., Chaicumpa, W.
    • Journal Title

      Journal of Infection 55

      Pages: 557-565

    • Description
      「研究成果報告書概要(和文)」より
    • Peer Reviewed
  • [Journal Article] Helicobacter pylori VacA Enhances Prostaglandin E2 Production through induction of Cyclooxygenase 2 Expression via a p38 Mitogen-Activated Protein Kinase/Activating Transcription Factor 2 Cascade in AZ-521 Cells.2007

    • Author(s)
      Hisatsune, J., Yamasaki, E., Nakayama, M., Shirasaka, D., Kurazono, H., Katagata, Y., Inoue, H., Han, J., Sap, J., Yahiro, K., Moss, J., Hirayama, T.
    • Journal Title

      Infect. Immun. 75

      Pages: 4472-4481

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Virulence genes of clinical Vibrio cholerae O1 isolates in Thailand and their ribotypes.2007

    • Author(s)
      Tapchaisri, P., Na-Ubol, M., Jaipaew, J., Srimanote, P., Chongsa-Nguan, M., Yamasaki, S., Hayashi, H., Kurazono, H., Chaicumpa, W.
    • Journal Title

      J. Infect. 55

      Pages: 557-565

    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] Purified Salmonella enterotoxin expresses cytotoxic activity to CHO Cells.2007

    • Author(s)
      Hisao Kurazono, Satomi Horie, Hidenobu Hoshi, Yoshiyuki Seto, Manas Chongsa-nguan, Wanpen Chicumpa, G. B. Nair, Soichi Makino, Toshiya Hirayama
    • Organizer
      The 42nd Joint Conference US-Japan Cooperative Medical Science Program
    • Place of Presentation
      Cholera Panel, Austin Texas
    • Year and Date
      20071205-07
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] Helicobacter pylori VacA Enhances PGE2 Production Through Inducti on of COX-2 Expression via a p38 MAP Kinase/ATF-2 Cascade in AZ-521 Cells2007

    • Author(s)
      Toshiya Hirayama, Jyunzo Hisatsune, Masaaki Nakayama, Hisao Kurazono, and Joel Moss:
    • Organizer
      The 42nd Joint Conference US-Japan Cooperative Medical Science Program
    • Place of Presentation
      Cholera Panel, Austin Texas
    • Year and Date
      20071205-07
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] Purified Salmonella enterotoxin expresses cytotoxic activity to CHO cells.2007

    • Author(s)
      Hisao Kurazono Satomi Horie, Hidenobu Hoshi, Yoshiyuki Seto, Manas Chongsa-nguan, Wanpen Chaicumpa, G.B. Nair, Souichi Makino, Toshiya Hirayama
    • Organizer
      The 42nd Joint Coference US-Japan Cooperative Medical Science Program, Cholera Panel
    • Place of Presentation
      Austin, Texas
    • Year and Date
      20071205-07
    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] Helicobacter pylori VacA enhances PGE2 production through induction of COX-2 expression via a p38 MAP kinase/ATF-2 cascade in AZ-521 cells.2007

    • Author(s)
      Toshiya Hirayama, Junzo Hisatsune, Masaaki Nakayama, Hisao Kurazono, and Joel Moss
    • Organizer
      The 42nd Joint Coference US-Japan Cooperative Medical Science Program, Cholera Panel
    • Place of Presentation
      Austin, Texas
    • Year and Date
      20071205-07
    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] サルモネラ・エンテロトキシン精製の試み2007

    • Author(s)
      倉園久生、星英之、牧野壮一
    • Organizer
      第80回日本細菌学会総会、
    • Place of Presentation
      大阪府、
    • Year and Date
      20070326-28
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] H. pylori VacAによるp38 MAP Kinase活性化依存COX-2発現誘導を介したPGE_2産生亢進作用2007

    • Author(s)
      久恒順三、倉園久生、片方陽太郎、平山壽哉
    • Organizer
      第80回日本細菌学会総会
    • Place of Presentation
      大阪府
    • Year and Date
      20070326-28
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] urified Salmonella enterotoxin expresses cytotxic activity to CHO Cells.2007

    • Author(s)
      Hisao Kurazono, Satomi Horie, Hidenobu Hoshi, Yoshiyuki Seto, Manas Chongsa-nguan, Wanpen Chicumpa, G. B. Nair, Soichi Makino, Toshiya Hirayama
    • Organizer
      平成19年度 日米医学協力会 コレラ・細菌性腸管感染症専門部会総会
    • Place of Presentation
      大阪大学 中之島センター、大阪
    • Year and Date
      2007-08-01
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] Helicobacter pylori VacA Enhances PGE2 Production Through Induction of COX-2 Expression via a p38 MAP Kinase/ATF-2 Cascade in AZ-521 Cells.2007

    • Author(s)
      Toshiya Hirayama, Jyunzo Hisatsune, Masaaki Nakayama, Hisao Kurazono, and Joel Moss:
    • Organizer
      平成19年度 日米医学協力会 コレラ・細菌性腸管感染症専門部会総会
    • Place of Presentation
      大阪大学 中之島センター、大阪
    • Year and Date
      2007-08-01
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] Escherichia coli from uncomplicated cystitis and from asymptomatic bacteriuria possess similar phylogeny, virulence genes and O serogroup profiles.2007

    • Author(s)
      Takahashi A, Kanamaru S, Kurazono H et al
    • Organizer
      アメリ力泌尿器科学会
    • Place of Presentation
      アナハイム,米国
    • Year and Date
      2007-05-20
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] Patial Purification of Salmonella enterotoxin.2006

    • Author(s)
      Yusuke Ohtani, Hidenobu Hoshi, Sou-ichi Makino, Manas Chongsa-nguan, Wanpen Chaicumpa, G.B. Nair, Hisao Kurazono
    • Organizer
      The 41st Joint Coference US-Japan Cooperative Medical Science Program, Cholera Panel
    • Place of Presentation
      Gifu, Japan
    • Year and Date
      20061105-07
    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] Clustering of Helicobacter pylori VacA in lipid rafts, mediated by its receptor, RPTPbeta, is required for intoxication and activation of intracellular signaling pathways in AZ-521 cells.2006

    • Author(s)
      Toshiya Hirayama, Masaaki Nakayama, Hisao Kurazono and Joel Moss
    • Organizer
      The 41st Joint Coference US-Japan Cooperative Medical Science Program, Cholera Panel
    • Place of Presentation
      Gifu, Japan
    • Year and Date
      20061105-07
    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] Helicobacter pylori VacA clustering in lipid rsfts, mediated by its receptor, RPTPbeta, is required for intoxication in AZ-521 cells.2006

    • Author(s)
      Toshiya Hirayama, Akihiro Wada, Hisao Kurazono, and Joel Moss
    • Organizer
      The 7th International workshop on Pathogenesis and Host response in Helicobacter Infections.
    • Place of Presentation
      Helsinger, Denmark
    • Year and Date
      20060701-04
    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] Salmonella enterotoxin2006

    • Author(s)
      Hisao Kurazono
    • Organizer
      The 11th Asian Conference on Diarrhoeal Diseases and Nutrition (11th ASCODD 2006)
    • Place of Presentation
      Bangkok, Thailand
    • Year and Date
      20060308-10
    • Description
      「研究成果報告書概要(欧文)」より

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Published: 2010-02-04  

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