2007 Fiscal Year Final Research Report Summary
IMMUNE RESPONSE TO GLYCOLIPIDS SEPARETED FROM MYCOBACTERIUM TUBERCULOSIS
| Project/Area Number |
18590436
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Bacteriology (including Mycology)
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| Research Institution | Kitasato University |
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Principal Investigator |
KUMAZAWA Yoshio Kitasato University, SCHOOL OF SCIENCE, PROFESSOR (30072375)
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| Co-Investigator(Kenkyū-buntansha) |
TAKIMOTO Hiroaki KITASATO UNIV., SCHOOL OF SCIENCE, LECTURER (00253534)
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| Project Period (FY) |
2006 – 2007
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| Keywords | Guinea pig / mycobacterial glycolipid / Cytokines / IL-12 / IFN-γ / monoclonal antibodies |
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| Research Abstract |
Chronic inflammation is the hallmark of pathogenesis of tuberculosis and due to the immune responses to not only protein antigens but also various glycolipids produced from Mycobacterium tuberculosis. The mechanisms by which mycobacterial lipid antigens present to T cells via CD1 family, especially CD1b molecule, have been yet unclear. Since guinea pigs carry CD1b molecule, we compared immune responses to protein and glycolipid antigens in guinea pigs. Recombinant guinea pig (gp) IL-12 p70 and IFN-γ were produced by using Sf9 cells transfected with baculo virus AcRP23-LacZ carrying gpIFN-γ, or gpIL-12p40 and gpIL-12p35 genes. We obtained biologically active rgpIFN-γ and rgplL-12 molecules. Using purified these proteins, mice were immunized for preparing monoclonal antibodies (mAb). We got several mAb clones to rgpIFN-γ and rgplL-12 which were able to neutralize the cytokine activy. We established a sandwich ELISA system for the detection and quantitation of IL-12 and IFN-γ.
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