2007 Fiscal Year Final Research Report Summary
Development of probiotics which regulate bacterial quorum sensing
| Project/Area Number |
18590437
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Bacteriology (including Mycology)
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| Research Institution | Kyorin University |
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Principal Investigator |
KAMIYA Shigeru Kyorin University, Faculty of Medicine, Professor (10177587)
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| Co-Investigator(Kenkyū-buntansha) |
OSAKI Takako Kyorin University, Faculty of Medicine, Instructor (90255406)
HANAWA Tomoko Kyorin University, Faculty of Medicine, Instructor (80255405)
KURATA Satoshi Kyorin University, Faculty of Medicine, Instructor (00383670)
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| Project Period (FY) |
2006 – 2007
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| Keywords | quorum sensing / probiotics / biofilm / intestinal flora / pathogenesis |
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| Research Abstract |
Quorum sensing (QS) is a system for regulation of bacterial gene by autoinducer (AI) which is produced according to bacterial density. Bacterial biofilm is associated with immunological escape, resistance to anti-microbial agents, and its formation is regulated by QS. In order to develop the probiotics which regulate bacterial quorum sensing, the effect of intestinal flora bacteria on biofilm formation by E. coil was examined.
Biofilm assay in microplate was established by using E. coll. Addition of AI-2 increased biofilm formation by E. coli in this assay. Using 16 species and 25 strains of intestinal flora bacteria, it was shown that culture supernatants of Bifidobacterium longm, Clostridium difficile, Enterococcus faecalis, Lactobacillus casei, Peptoniphilus asaccharolyticus and Veillonella parvula inhibited biofilm formation by E. coil. In contrast, culture supernatants of Bcateroides and Fusobacterium necrophorum enhanced biofilm formation. As there was no significant difference in biofilm formation between Helicobacter pylori TK1402 stain and its luxS mutant strain without AI-2 production, it was speculated that biofilm formation was not induced in IL pylori by QS.
Using 576 strains isolated from feces of healthy adults, the effects of culture supernatants of these bacteria on biofilm formation by E. coli was examined. Twelve strains inhibited the biofilm formation with less than 80 % level of control. As biofilm formation by E. coli was not effectively done, the additional experiment using 534 stains was performed. Culture supernatants of 43 and 22 strains inhibited biofilm formation by E. coli by more than 20% and 50%, respectively. Culture supernatants of 42 and 5 strains inhibited biofilm formation by S. aureus by more than 205 and 50%, respectively. Identification of these strains and the effects of these stains on the expression of QS-related genes for adhesin and bacterial toxin need to be clarified.
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[Journal Article] The efficacy of Clostridium butyricum preparation concomitantly with Helicobacter pylori eradication therapy in relation to changes in the intestinal microflora2008
Author(s)
Imase, K., Takahashi, M., Tanaka, A., Tokunaga, K., Sugano, H., Tanaka, M., Ishida, H., Kamiya, S., Takahashi, S
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Journal Title
Microbiol Immunol 52 (1)
Pages: 1-6
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Plasmodium berghei XAT : Contribution of gammadelta T cells to host defense against infection with blood-stage nonlethal malaria parasite2007
Author(s)
Kobayashi, F., Niikura, M., Waki, S., Matsui, T., Fujino, T., Tsuruhara, T., Kamiya, S
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Journal Title
Exp Parasitol 117 (4)
Pages: 368-375
Description
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[Journal Article] Antifungal drug susceptibility of Cryptococcus neoformans from clinical sources in Nairobi, Kenya2007
Author(s)
Bii, CC., Makimura, K., Abe, S., Taguchi, H., Mugasia, OM., Revathi, G., Wamae, NC., Kamiya, S
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Journal Title
Mycoses 50 (1)
Pages: 25-30
Description
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[Journal Article] Mutation of luxS affects motility and infectivity of Helicobact er pylori in gastric mucosa of Mongolian gerbil model2006
Author(s)
Osaki T, Hanawa T, Manzoku T, Fukuda M, Kawakami H, Suzuki H, Yamaguchi H, Yen X, Taguchi H, Kurata S, Kamiya S
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Journal Title
J Med Microbiol 55(11)
Pages: 1477-1485
Description
「研究成果報告書概要(和文)」より
Peer Reviewed
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[Journal Article] Mutation of luxS affects motility and infectivity of Helicobacter pylori in gastric mucosa of Mongolian gerbil model2006
Author(s)
Osaki, T., Hanawa, T., Manzoku, T., Fukuda, M., Kawakami, H., Suzuki, H., Yamaguchi, H., Yen, X., Taguchi, H., Kurata, S., Kamiya, S
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Journal Title
J Med Microbiol 55 (11)
Pages: 1477-1485
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] In vitro antibacterial activity of a novel antimicrobial agent TG44 for treatment of Helicobacter pylori2006
Author(s)
Kamoda, O., Anzai, K., Mizoguchi, J., Yanagi, T., Shioiri, M., Nishio, T., Kamiya, S
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Journal Title
Antimicrob Agents Chemother 50 (9)
Pages: 3062-3069
Description
「研究成果報告書概要(欧文)」より
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