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2007 Fiscal Year Final Research Report Summary

Structural analysis for enzymatic activity of sphingomyelinase from Bacillus cereus

Research Project

Project/Area Number 18590441
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Bacteriology (including Mycology)
Research InstitutionTokushima Bunri University

Principal Investigator

SAKURAI J.  Tokushima Bunri University, Fac. Pharm. Sci., Full Professor (80029800)

Co-Investigator(Kenkyū-buntansha) KOBAYASHI Keiko  Tokushima Bunri University, Fac. Pharm. Sci., assistant Professor (90170315)
ODA M.  Tokushima Bunri University, Fac. Pharm. Sci., assistant Professor (00412403)
Project Period (FY) 2006 – 2007
KeywordsBacillus cereus / sphingomyelinase / hemolytic activity / structural analysis / divalent cation / cleft / 3D structure / neutral SMase
Research Abstract

Neutral sphingomyelinase (nSMase) has been implicated in the generation of second messengers in mammalian cells. Bacillus cereus Smase (Bc-SMase) mimics the action of the endogenous mammalian nSMase in causing differentiation, development aging and apoptosis. The enzymatic activity of Bc-SMase was activated in the order of Co^<2+> > Mg^<2+> >> Ca^<2+>. The crystal structures of Bc-Smase with these metal ions were determined. The water-bridged twin divalent metal ions, Co^<2+> and Mg^<2+>, at the center of cleft were concluded to be the catalytic architecture to exert the sphingomyelinase activity. On the other hands, the architecture of Ca^<2+> binding at the site was different from that of Ca^<2+> and Mg^<2+>. The other binding site of these metal ions was at one side edge of the cleft. The crystal structure of the enzyme with Mg^<2+> or Co^<2+> at the site provides the universal structure bases for the hydrolytic activity of nSMase. In addition, the structural features and site-directed mutagenesis suggest that the specific-hairpin with the aromatic amino acid residues, the new active site, involves in binding to SM and membrane SM.

  • Research Products

    (13 results)

All 2008 2007 2006

All Journal Article (7 results) (of which Peer Reviewed: 3 results) Presentation (5 results) Book (1 results)

  • [Journal Article] The relationship between the metabolism of sphingomyelin species and hemolysis of sheep erythrocytes induced by Clostridium perfringens alpha-toxin2008

    • Author(s)
      M.Oda, T.Matsuno, R.Shiihara, S.Ochi, R.Yamauchi, Y.Saito, H.Imagawa, M.Nagahama, M.Nishizawa and J.Sakurai
    • Journal Title

      J.Lipid Res. (In press)

    • Description
      「研究成果報告書概要(和文)」より
    • Peer Reviewed
  • [Journal Article] The relationship between the metabolism of sphingomyelin species and hemolysis of-sheep erythrocytes induced by Clostridium pelfringens alpha-toxin.2008

    • Author(s)
      M. Oda, T. Matsuno, R. Shiihara, S. Ochi, R. Yamauchi, Y. Saito, H. Imagawa, M. Nagahama, M. Nishizawa, J. Sakurai.
    • Journal Title

      J. Lipid Res. (In press)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Effect of unsaturated bonds in the sn-2 acyl chain of phosphatidyl-choline on the membrane-damaging action of Clostridium perfringens alpha-toxin toward liposomes2007

    • Author(s)
      M.Nagahama, A.Otsuka, M.Oda, R.K.Singh, Z.M.Zyta, H.Imagawa, M.Nishizawa, and J.Sakurai
    • Journal Title

      Biochim.Biophys.Acta-Biomembrane 1768

      Pages: 2940-2945

    • Description
      「研究成果報告書概要(和文)」より
    • Peer Reviewed
  • [Journal Article] Effect of unsaturated bonds in the sn-2 acyl chain of phosphatidylcholine on the membrane-damaging action of Clostridium perfringens alpha-toxin toward liposomes.2007

    • Author(s)
      M. Nagahama, A. Otsuka, M. Oda, R. K. Singh, Z. M. Zyta, H. Imagawa, M. Nishizawa, J. Sakurai
    • Journal Title

      Biochim. Biophys. Acta 1768

      Pages: 2940-2945

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Structural basis of the sphingomyelin phosphodiesterase activity in natural sphingomyelase from Bacillus cereus2006

    • Author(s)
      H.Ago, M.Oda, M.Takahashi, H.Tsuge, S.Ochi, N.Katunuma, M.Miyano and J.Sakurai
    • Journal Title

      J.Biol.Chem. 281

      Pages: 16157-16167

    • Description
      「研究成果報告書概要(和文)」より
    • Peer Reviewed
  • [Journal Article] Structural basis of the sphingomyelin phosphodiesterase activity in natural Sphingomyelase frm Bacillus cereus2006

    • Author(s)
      H. Ago, M. Oda, M. Takahashi, H. Tsuge, S. Ochi, N. Katunuma, M. Miyano, J. Sakurai.
    • Journal Title

      J. Biol. Chem. 281

      Pages: 16157-1616

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Oligomerization of Clostridium perfringens E -toxin is dependent upon membrane fluidity in liposomes. M. Nagahama2006

    • Author(s)
      H. Hara, M. Fernandez-Miyakawa, Y. Itohayashi, J. Sakurai
    • Journal Title

      Biochemistry 45

      Pages: 296-302

    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] セレウス菌スフィンゴミエリナーゼの細胞膜結合領域の解析2007

    • Author(s)
      橋本 学, 小田 真隆, 津下 英明, 勝沼 信彦, 永浜 政博, 櫻井 純
    • Organizer
      第60回日本細菌学会中国・四国支部総会
    • Place of Presentation
      岡山
    • Year and Date
      20071000
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] Oligomerization of Clostridium perfringens epsilon-toxin is dependent on membrane fluidity in liposomes.2006

    • Author(s)
      OM. Nagahama, K. Kobayashi, H. Hara, M. Fernandes Miyakawa, J. Sakurai
    • Organizer
      Clostpath
    • Place of Presentation
      Nottingham, UK
    • Year and Date
      20060600
    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] Clostridium perfringens alpha-toxin induces superoxide anion generation in rabbit neutrophils.2006

    • Author(s)
      OM. Oda, S. Ikari, T. Matsuno, Y. Morimune, J. Sakurai
    • Organizer
      Clostpath
    • Place of Presentation
      Nottingham, UK
    • Year and Date
      20060600
    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] Clostridium perfringens alpha-toxin induces superoxide anion generation in rabbit neutrophils.2006

    • Author(s)
      OM. Oda, J. Sakurai
    • Organizer
      The 8th Japan-Korea International Symposium on Microbiology
    • Place of Presentation
      Kanazawa, Japan
    • Year and Date
      20060300
    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] Role of Tyrosin-57 and-65 in biological and sphingomyelinase activities of Clostridium perfringens alpha-toxin.2006

    • Author(s)
      OM. Nagahama, A. Otsuka, K. Kobayashi, J. Sakurai
    • Organizer
      The 8th Japan-Korea International Symposium on Microbiology
    • Place of Presentation
      Kanazawa, Japan
    • Year and Date
      20060300
    • Description
      「研究成果報告書概要(欧文)」より
  • [Book] スタンダード薬学シリーズ5感染症の現状とその予防2007

    • Author(s)
      櫻井 純
    • Total Pages
      457
    • Publisher
      東京化学同人
    • Description
      「研究成果報告書概要(和文)」より

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Published: 2010-02-04  

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