2007 Fiscal Year Final Research Report Summary
Analysis of virus adaptations in the novel HCV cell culture and infection system
| Project/Area Number |
18590462
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Virology
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| Research Institution | National Institute of Infectious Diseases |
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Principal Investigator |
MURAYAMA Asako National Institute of Infectious Diseases, Department of Virology II, Scientist (40415534)
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| Co-Investigator(Kenkyū-buntansha) |
WAKITA Takaji National Institute of Infectious Diseases, Department of Virology II, Director (40280789)
DATE Tomoko National Institute of Infectious Diseases, Department of Virology II, Scientist (40392360)
MORIKAWA Kenichi National Institute of Infectious Diseases, Department of Virology II, Scientist (60384377)
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| Project Period (FY) |
2006 – 2007
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| Keywords | HCV / Virus / Virus replication / adaptation / Infection |
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| Research Abstract |
We have established HCV culture system using JFH-1 strain isolated from a fulminant hepatitis patient. This system enabled us to analyze HCV life cycles in detail. In this research project, we have analyzed how HCV adapted in cultured cells and organisms. We have also tried to determine which virus components involved in HCV adaptation.
HCV adaptation in cultured cells: we transfected in vitro-synthesized HCV RNA into Huh7 cells and collected infectious HCV particles. HCV infection sometimes causes plaque formation. We collected plaque forming HCVs, and infected to naive Huh7 cells. Isolated virus subclones from individual plaque showed greater replication efficiency and cytopathogenicity than parental virus. We analyzed the sequence of this HCV and found several nucleotide mutations with amino acid substitution in NS5B coding region. These mutations may be involved in plaque forming ability and virus genome replication.
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[Journal Article] A Critical Role of Virion-Associated Cholesterol and Sphingolipid in Hepatitis C Virus Infection.2008
Author(s)
Aizaki H, Morikawa K, Fukasawa M, Hara H, Inoue Y, Tani H, Saito K, Nishijima M, Hanada K, Matsuura Y, Lai MM, Miyamura T, Wakita T, Suzuki T.
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Journal Title
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Development of plaque assays for hepatitis C virus-JFH1 strain and isolation of mutants with enhanced cytopathogenicity and replication capacity.2008
Author(s)
Sekine-Osajima Y, Sakamoto N, Mishima K, Nakagawa M, Itsui Y, Tasaka M, Nishimura-Sakurai Y, Chen CH, Kanai T, Tsuchiya K, Wakita T, Enomoto N, Watanabe M.
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Journal Title
Virology. 5 ; 371(1)
Pages: 71-85
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Hepatitis C virus non-structural proteins responsible for suppression of the RIG-I/Cardif-induced interferon response.2007
Author(s)
Tasaka M, Sakamoto N, Itakura Y, Nakagawa M, Itsui Y, Sekine-Osajima Y, Nishimura-Sakurai Y, Chen CH, Yoneyama M, Fujita T, Wakita T, Maekawa S, Enomoto N, Watanabe M.
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Journal Title
J Gen Virol. 88(Pt12)
Pages: 3323-33
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Scavenger receptor class B type I is a key host factor for hepatitis virus infection required for an entry step closely linked to CD81.2007
Author(s)
Zeisel MB, Koutsoudakis G, Schnober EK, Haberstroh A, Blum HE, Cosset FL, Wakita T, Jaeck D, Doffoel M, Royer C, Soulier E, Schvoerer E, Schuster C, Stoll-Keller F, Bartenschlager R, Pietschmann T, Barth H, Baumert TF.
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Journal Title
Hepatology. 46(6)
Pages: 1722-31
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] The NS3 helicase and NS5B-to-3'X regions are important for efficient hepatitis C virus strain JFH-1 replication in Huh7 cells.2007
Author(s)
Murayama A, Date T, Morikawa K, Akazawa D, Miyamoto M, Kaga M, Ishii K, Suzuki T, Kato T, Mizokami M, Wakita T.
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Journal Title
J Virol 81(15)
Pages: 8030-40
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Infection of human hepatocyte chimeric mouse with genetically engineered hepatitis C virus and its susceptibility to interferon.2007
Author(s)
Hiraga N, Imamura M, Tsuge M, Noguchi C, Takahashi S, Iwao E, Fujimoto Y, Abe H, Maekawa T, Ochi H, Tateno C, Yoshizato K, Sakai A, Sakai Y, Honda M, Kaneko S, Wakita T, Chayama K.
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Journal Title
FEBS Lett. 15 ; 581(10)
Pages: 1983-7
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Upregulation of indoleamine 2, 3-dioxygenase in hepatitis C virus infection.2007
Author(s)
Larrea E, Riezu-Boj JI, Gil-Guerrero L, Casares N, Aldabe R, Sarobe P, Civeira MP, Heeney JL, Rollier C, Verstrepen B, Wakita T, Borras-Cuesta F, Lasarte JJ, Prieto J.
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Journal Title
J Virol 81(7)
Pages: 3662-6
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] E6AP ubiquitin ligase mediates ubiquitylation and degradation of hepatitis C virus core protein.2007
Author(s)
Shirakura M, Murakami K, Ichimura T, Suzuki R, Shimoji T, Fukuda K, Abe K, Sato S, Fukasawa M, Yamakawa Y, Nishijima M, Moriishi K, Matsuura Y, Wakita T, Suzuki T, Howley PM, Miyamura T, Shoji I.
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Journal Title
J Virol 81(3)
Pages: 1174-85
Description
「研究成果報告書概要(欧文)」より
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[Presentation] Critical roles of virion-associated cholesterol and sphingolipids in the viral infectivity2007
Author(s)
H Aizaki, M Fukazawa, K Morikawa, H Hara, H Tani, K Hanada, Y Matsuura, M Lai, T Miyamura, T Wakita, T Suzuki
Organizer
14th International Meeting on Hepatitis C and Related Viruses.
Place of Presentation
Glasgow, Scotland, UK
Year and Date
20070909-13
Description
「研究成果報告書概要(欧文)」より
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[Presentation] Characterization of infectious HCV particles produced from various liver-derived cell lines2007
Author(s)
Akazawa D, Takahashi H, Omi N, Date T, Morikawa K, Murayama A, Nakamura N, Mochizuki H, Wakita T
Organizer
14th International Meeting on Hepatitis C and Related Viruses.
Place of Presentation
Glasgow, Scotland, UK
Year and Date
20070909-13
Description
「研究成果報告書概要(欧文)」より
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