• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to project page

2007 Fiscal Year Final Research Report Summary

Deve lopment of assay systems for detection anti-mouse antibody

Research Project

Project/Area Number 18590534
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Laboratory medicine
Research InstitutionSapporo Medical University

Principal Investigator

TSUJI Naoki  Sapporo Medical University, SCHOOL OF MEDICINE, ASSISTANT PROFESSOR (00347171)

Co-Investigator(Kenkyū-buntansha) WATANABE Naoki  SAPPORO MEDICAL UNIVERSITY, SCHOOL OF MEDICINE, Professor (10158644)
Project Period (FY) 2006 – 2007
KeywordsANTI-ANIMAL ANTIBODY / HAMA / ELISA FOR DETECTIONOF HAMA / HETEROPHILIC BLOCKING REAGENT
Research Abstract

In the present study, we developed enzyme-linked immunosorvent assay (ELISA) for the detection of human anti-mouse antibodies (HAMA) in the serum and investigate the frequency of people possesses HAMA in Japan.
34 cases out of 290 cases (11.7%) were positive (IgG type ; 18 cases, IgM type 20 cases). The most frequent IgG subtype that HAMA recognized was IgG1 (IgG type ; 14 cases, IgM type 11 cases). In addition, some cases showed positive against two or three kinds of IgG subtype (IgG type ; IgG2a+IgG2b:3 rases, IgG1+IgG2a+IgG2b:1ca.p, IgM type ; IgG2a+IgG2b:5 cases, IgG1+IgG2a + IgG2b:2 case).
To eliminate the effect of HAMA on the immunoassays, heterophilic blocking reagent (HBR) which contains mouse immunoglobulins is added to sample or assay reagents. We then determined the effect of HBR on HAMA using ETISA system. When 10 or 15μl of HBR was added to 100 ul of sample, measured values were decreased to 30-50% of untreated samples. In contrast, when of HBR was added to sample, measured values were significantly increased 2-3 times of untreated samples. These results indicate that establishing the amount of HBR added to samples is important to block the effect of HAMA on the immunoassays.

  • Research Products

    (18 results)

All 2007 2006

All Journal Article (14 results) (of which Peer Reviewed: 7 results) Presentation (4 results)

  • [Journal Article] Specific overexpression of OLFM4(GW112/HGC-1) mRNA incolon, breast and lung cancer tissues detected using quantitative analysis2007

    • Author(s)
      Koshida S, et. al.
    • Journal Title

      Cancer Sci 98

      Pages: 315-320

    • Description
      「研究成果報告書概要(和文)」より
    • Peer Reviewed
  • [Journal Article] 01 factomedin 4 promotes S-phase transition in proliferation of pancreatic cancer cells2007

    • Author(s)
      Kobayashi D, et. al.
    • Journal Title

      Cancer Sci 98

      Pages: 334-340

    • Description
      「研究成果報告書概要(和文)」より
    • Peer Reviewed
  • [Journal Article] 15-Deoxy-Delta(12,14)-prostaglandin J(2) inhibits G(2)-M phaseprogression in human breast cancer cells via the down-regulation of cyclinB1 and survivin expression2007

    • Author(s)
      Kamagata C, et. al.
    • Journal Title

      Breast Cancer Res Treat 102

      Pages: 263-273

    • Description
      「研究成果報告書概要(和文)」より
    • Peer Reviewed
  • [Journal Article] Diagnostic relevance of overexpressed mRNA of novel oncogene with kinase-domain( NOK) in lung cancers2007

    • Author(s)
      Amachika T, et. al.
    • Journal Title

      Lung Cancer 56

      Pages: 337-340

    • Description
      「研究成果報告書概要(和文)」より
    • Peer Reviewed
  • [Journal Article] Survivin down-regulation plays acrucial role in 3-hydroxy-3-methyl-glutaryl coenzyme A reductase inhibitor-induced apoptosis in cancer2007

    • Author(s)
      Kaneko R, et. al.
    • Journal Title

      J Biol Chem 282

      Pages: 19273-81

    • Description
      「研究成果報告書概要(和文)」より
    • Peer Reviewed
  • [Journal Article] Inhibition of estrogen receptor beta-mediated human telomerase reversetranscriptase gene transcription via the suppression of mitogen-.activated protein kinase signaling plays an important role in 15-deoxy-Delta(12,14)-prostaglandin J(2)-induced apoptosis in cancer cells.2007

    • Author(s)
      Kondoh K, et. al.
    • Journal Title

      Exp Cell Res 313

      Pages: 3486-3496

    • Description
      「研究成果報告書概要(和文)」より
    • Peer Reviewed
  • [Journal Article] Specific overexpression of OLFM4GW112/hGC-1 mRNA in colon, breast, and lung cancer tissues detected using quantitative analysis2007

    • Author(s)
      Koshida, S., Kobayashi, D., Moriai, R., Tsuji, N., Watanabe, N
    • Journal Title

      Cancer Sci 98

      Pages: 315-320

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Olfactomedin 4 promotes S-phase transition in proliferation of pancreatic cancer cells2007

    • Author(s)
      Kobayashi, D., Koshida, S., Moriai, R., Tsuji, N., Watanabe, N
    • Journal Title

      Cancer Sci 98

      Pages: 334-340

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] 15-Deoxy-Delta(12,14)-prostaglandin J(2) inhibits G(2)-M phase progression in human breast cancer cells via the down-regulation of cyclin B1 and survivn expression2007

    • Author(s)
      Kamagata, C., Tsuji, N., Moriai, M., Kobayashi, D., Watanabe, N
    • Journal Title

      Breast Cancer Res Treat 102

      Pages: 263-273

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Diagnostic relevance of overexpressed mRNA of novel oncogene with kinase-domain (NOK) in lung cancers2007

    • Author(s)
      Amachika, T., Kobayashi, D., Moriai, R., Tsuji, N., Watanabe, N
    • Journal Title

      Lung Cancer 56

      Pages: 337-340

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Survivin down-regulation plays a crucial role in 3-hydroxy-3-methylglutaryl coenzyme A reductase inhibitor-induced apoptosis in cancer2007

    • Author(s)
      Kaneko, R., Tsuji, N., Asanuma, K., Tanabe, H., Kobayashi, D., Watanabe, N
    • Journal Title

      J Biol Chem 282

      Pages: 19273-19281

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] In hibition of estrogen receptor β -mediated human telomerase reverse transcriptase gene transcription via the suppression of mitogen-activated protein kinase signaling plays an important role in 15-deoxy-Δ12, 14-prostaglandin J2-induced apoptosis in cancer cells2007

    • Author(s)
      Kondo, K., Tsuji, N., Asanuma, K., Kobayashi, D., Watanabe, N
    • Journal Title

      Exp Cell Res 313

      Pages: 3486-3496

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Diagnostic relevance of overexpressed NOK mRNA in breast cancer2006

    • Author(s)
      Moriai R, et. al.
    • Journal Title

      Anticancer Res 26

      Pages: 4969-4973

    • Description
      「研究成果報告書概要(和文)」より
    • Peer Reviewed
  • [Journal Article] Diagnostic relevance of overexpressed NOK mRNA in breast cancer2006

    • Author(s)
      Moriai, R., Kobayashi, D., Amachika, T., Tsuji, N., Watanabe, N
    • Journal Title

      Anticancer Res 26

      Pages: 4969-4974

    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] Symposium "Update knowledge of cancer research and impact on clinical laboratory. Functional analysis of anti-apoptosis molecule Survivin and application for cancer diagnosis and treatment2007

    • Author(s)
      Tsuji, N., Watanabe, N
    • Organizer
      The 54th annual congress of Japanese Society of Laboratory Medicine
    • Place of Presentation
      Osaka
    • Year and Date
      20070000
    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] 癌研究の新しい知見と臨床検査へのインパクト-分担-抗アペトーシス分子Survivinの機能解析と癌診断・治療への応用2007

    • Author(s)
      辻 直樹
    • Organizer
      第54回日本臨床検査医学会学術集会
    • Place of Presentation
      大阪国際会議場
    • Year and Date
      2007-11-23
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] Symposium "How far do we know the molecular function of tumor marker? Molecular function of anti-apoptosis molecule Survivin"2006

    • Author(s)
      Tsuji, N., Watanabe, N
    • Organizer
      The 38th annual congress of The Japan Society for Clinical Laboratory Automation
    • Place of Presentation
      Kobe, Japan
    • Year and Date
      20060000
    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] 腫瘍マーカーの分子機能はどこまで解っているのか-分担-抗アポトーシス分子Survivinの分子機能2006

    • Author(s)
      辻 直樹
    • Organizer
      日本臨床検査自動化学会第38回大会
    • Place of Presentation
      神戸国際会議場
    • Year and Date
      2006-10-13
    • Description
      「研究成果報告書概要(和文)」より

URL: 

Published: 2010-02-04  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi