2007 Fiscal Year Final Research Report Summary
Understanding the pathophysiology of taste disorders by analysis of taste receptor gene expression in the tongue of patients with taste disorders.
| Project/Area Number |
18590541
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Laboratory medicine
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| Research Institution | Nihon University |
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Principal Investigator |
IKEDA Minoru Nihon University, School of Medicine, professor (30130420)
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| Co-Investigator(Kenkyū-buntansha) |
KOKUBUN Shinichiro Nihon University, School of Medicine, Professor (20153520)
TAKAO Kyoichi Nihon University, School of Medicine, Research Assistant (90187922)
ONODA Keiko Nihon University, School of Medicine, Assistant Professor (00349995)
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| Project Period (FY) |
2006 – 2007
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| Keywords | taste-related gene / T2R Family / taste disorder / human / taste disorder due to zinc deficiency / spontaneous abnormal taste |
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| Research Abstract |
In this study, we examined the expression of ten genes (TAS2R40, 42, 43, 48, T2R3, 8, 9, 10, 13, and 16) related to the bitter taste receptor in healthy control subjects and in patients with taste disorders. I suggest changing the underlined to" Taste receptor gene expression was examined using RT-PCR. Total RNA was isolated from a sample scraped from the foliate papillae using TRIzol (Invitrogen). The total RNA was reverse transcribed using Superscript III (Invitrogen). The cDNA was subjected to PCR using ExTaq (Takara), and the PCR products were analyzed using a Bioanalyzer 2100 (Agilent).
Patients with taste disorders showed a lower taste receptor gene expression than that of healthy control subjects, with TAS2R40 expressed at significantly lower levels. When considering the causes of taste disorders, cases of taste disorder due to zinc deficiency in particular tended to show lower expression of taste receptor genes. In these cases, TAS2R40 and T2R8 and 10 were expressed at significantly lower levels. TAS2R40 was expressed at significantly lower levels in cases of idiopathic taste disorder, whereas T2R3 showed significantly lower expression in cases with drug-induced taste disorder. Cases with bitter taste disorder showed lower expression of these taste genes compared to that of healthy control subjects. For TAS2R40, 42, T2R3,8 and 9, the lower expression was statistically significant.
These results suggest that that TAS2R40, 42, T2R3, 8, 9and 10 are likely to be related to taste in humans. In addition, these findings can be applied to the diagnosis of taste disorders and in the determination of a prognosis. Clinical application appears most appropriate in cases with taste disorder due to zinc deficiency.
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