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2007 Fiscal Year Final Research Report Summary

Clinical Chemistry of Newly-found Esterase for Endogenous Digitalis-like Factor

Research Project

Project/Area Number 18590543
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Laboratory medicine
Research InstitutionKansai Medical University

Principal Investigator

KOMIYAMA Yutaka  Kansai Medical University, Faculty of Medicine, Assistant professor (40140264)

Co-Investigator(Kenkyū-buntansha) TAKAHASHI Hakuo  Kansai Medical University, Faculty of Medicine, Professor (80094431)
Project Period (FY) 2006 – 2007
Keywordsendogenous digitalis-like factor / bufadienolide / cardenolides / marinobufotoxin / renin / angiotensin / esterase / cardiotonic steroid
Research Abstract

Endogenous digitalis-like factor (EDLF) act as important modulator for water and salt metabolism. From recent studies endogenous digitalis-like factor is consisted of cardenolides and bufadienolides. Recently we found marinobufotoxin (MBT) as the new member of EDLF. The structure of this compound was confirmed by tandem mass spectrometric signals of liquid chromatography-mass spectrometry and nuclear magnetic resonance (NMR) analysis. Interestingly this compound consists of cardiotonic steroid with C6-chain and amino acids, argentine. And the esterase for this side chain was searched. The reaction of this esterase mimicked by the tissue kallikrein and other esterases such as cholesterol esterase were relatively slow and the physiological meanings are still ambiguous. But we found the contribution of rennin-angiotensin system (RAS) and MBT on hypertension with use of SHR rats. In the angiotensin-receptor blocker (ARB), telmisartan group, the plasma level of MBT was lower than that in the control group. The urine MBT excretion increase after 2 and 4 weeks but the MBT excretion modified by the ARB did not increase and the blood pressure did not increase. These results suggested that MBT contributed the control mechanism in SHR with RAS. And this analytic system was also applied to clinical toxicology area.

  • Research Products

    (22 results)

All 2009 2008 2007 Other

All Journal Article (10 results) (of which Peer Reviewed: 2 results) Presentation (12 results)

  • [Journal Article] 西日本3施設における薬毒物分析機器稼働の現状と課題 集団中毒に対する危機管理体制2009

    • Author(s)
      小宮山豊, 原克子, 高橋伯夫, 津田紀子, 石部琢也, 丸山克之, 穂坂直美, 八尋美保子, 秀島里沙, 眞名子順一
    • Journal Title

      中毒研究 22

      Pages: 12-18

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] The present situation and problem of the analytic instruments of clinical toxicology laboratory in hospital. The experience of three laboratory in west Japan.2009

    • Author(s)
      Komiyama Y, Hara K, Takahashi H, Tsuda N, Ishibe T, Maruyama K, Hosaka N, Yahiro M, Hideshima R, Manago J
    • Journal Title

      Chudoku Kenkyu 22

      Pages: 12-18

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] 腎不全とホルモン1. 慢性腎臓病(CKD)の増悪・進展に関与するホルモン内因性ジギタリス様物質.2008

    • Author(s)
      高橋伯夫
    • Journal Title

      ホルモンと臨床 56

      Pages: 387-395

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] 高血圧性心不全-病態と診断・治療の現状高血圧性心不全と交感神経系2008

    • Author(s)
      高橋伯夫
    • Journal Title

      血圧 15-2

      Pages: 140-143

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Hypertension-induced heart failure and sympathetic nerve system2008

    • Author(s)
      Hakuo Takahashi
    • Journal Title

      Ketsuatsu 15

      Pages: 140-143

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Endogenous Digitalis Like Factor2008

    • Author(s)
      Hakuo Takahashi
    • Journal Title

      Horumon to Rinsho 56

      Pages: 387-395

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Novel digitalis-like factor, marinobufotoxin, isolated from cultured Y-1 cells, and its hypertensive effect in rats.2007

    • Author(s)
      Yoshika M, Komiyama Y, Konishi M, Akizawa T, Kobayashi T, Date M, Kobatake S, Masuda M, Masaki H, Takahashi H
    • Journal Title

      Hypertension 49

      Pages: 209-214

    • Description
      「研究成果報告書概要(和文)」より
    • Peer Reviewed
  • [Journal Article] Novel digitalis-like factor, marinobufotoxin, isolated from cultured Y-l cells, and its hypertensive effect in rats.2007

    • Author(s)
      Yoshika M, Komiyama Y, Konishi M, Akizawa T, Kobayashi T, Date M, Kobatake S, Masuda M, Masaki H, Takahashi H
    • Journal Title

      Hypertension 49

      Pages: 209-214

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] 自然発症高血圧ラットにおける内因性ジギタリス様物質, マリノブッォトキシンの動態 -血圧調節におけるレニン-アンジオテンシン系の関与について-

    • Author(s)
      吉賀正亨, 小宮山豊, 高橋伯夫
    • Journal Title

      心臓 (印刷中)

    • Description
      「研究成果報告書概要(和文)」より
    • Peer Reviewed
  • [Journal Article] An endogenous digitalis-like factor, marinobufotoxin, in spontaneously hypertension rats-involvement of reninangiotensin system in blood pressure regulation-

    • Author(s)
      Masamichi Yoshika, Yutaka Komiyama, Hakuo Takahashi
    • Journal Title

      Shinzo (in printing)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] 副腎髄質における内因性ジギタリス様物質であるマリノブフォトキシンとアンジオテンシンについての検討2008

    • Author(s)
      吉賀正亨, 小宮山豊, 高橋伯夫
    • Organizer
      第31回日本高血圧学会総会
    • Place of Presentation
      札幌
    • Year and Date
      20081000
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] The effect of endo- genous digitalis, marinobufotoxin and angiotensin in rat adrenal gland2008

    • Author(s)
      M Yoshika, Y Komiyama, H Takahashi
    • Organizer
      The 31^<st> Japanese congress for hyper- tension
    • Place of Presentation
      Sapporo
    • Year and Date
      20081000
    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] The Effect of Endogenous Digitalis, Marinobufotoxin, in Sodium Metabolism2008

    • Author(s)
      M Yoshika, Y Komiyama, H Takahashi
    • Organizer
      The 14^<th> International Symposium for SHR rat
    • Place of Presentation
      Puraha, Czech Republic
    • Year and Date
      20080700
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] 西日本3施設における薬毒物分析機器稼働の現状と課題2008

    • Author(s)
      小宮山豊, 原克子, 高橋伯夫, 津田紀子, 丸山克之, 坂田育弘, 穂坂直美, 八尋美保子, 秀島里沙, 眞名子順一
    • Organizer
      第30回日本中毒学会 シンポジウム
    • Place of Presentation
      和歌山
    • Year and Date
      20080700
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] The Effect of Endogenous Digital is, Marinobufotoxin, in Sodium Metabolism2008

    • Author(s)
      Yoshika, Y Komiyama, H Takahashi
    • Organizer
      The 14^<th> International Symposium for SHR rat
    • Place of Presentation
      Puraha
    • Year and Date
      20080700
    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] The present situation and problem of the analytic instruments of clinical toxicology laboratory in hospital. The experience of three laboratory in west Japan.2008

    • Author(s)
      Komiyama Y, Hara K, Takahashi H, Tsuda N, Ishibe T, Maruyama K, Hosaka N, Yahiro M, Hideshima R, Manago J
    • Organizer
      The 30^<th> Japanese congress for clinical toxicology
    • Place of Presentation
      Wakayama
    • Year and Date
      20080700
    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] 内因性ジギタリス様物質であるマリノブッォトキシンの食塩負荷したラットおよび培養細胞における検討2007

    • Author(s)
      吉賀正亨, 小宮山豊, 高橋伯夫
    • Organizer
      第43回高血圧関連疾患モデル学会
    • Place of Presentation
      大阪
    • Year and Date
      20071100
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] 下垂体培養細胞GH3における内因性ジギタリス様物質(マリノブフォトキシン)の分泌動態の検討2007

    • Author(s)
      吉賀正亨, 小宮山豊, 高橋伯夫
    • Organizer
      第54回日本臨床検査医学会学術集会
    • Place of Presentation
      大阪
    • Year and Date
      20071100
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] The effect of endo- genous digitalis, marinobufotoxin, on therat and cultured cells with salt loading.2007

    • Author(s)
      M Yoshika, Y Komiyama, H Takahashi
    • Organizer
      The 43rd Japanese conference of hyper- tension and related-model rats
    • Place of Presentation
      Osaka
    • Year and Date
      20071100
    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] The secretion of endo- genous digitalis, marinobufotoxin, in cultured cell, GH3, from hypothalamus2007

    • Author(s)
      M Yoshika, Y Komiyama, H Takahashi
    • Organizer
      The 54^<th> cogress for Japanese society of laboratory medicine
    • Place of Presentation
      Osaka
    • Year and Date
      20071100
    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] 食塩負荷ラットにおける新規内因性ジギタリス様物質であるマリノブフォトキシンの動態2007

    • Author(s)
      吉賀正亨, 小宮山豊, 高橋伯夫
    • Organizer
      第30回日本高血圧学会総会
    • Place of Presentation
      那覇
    • Year and Date
      20071000
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] The effect of endo- genous digitalis, marinobufotoxin, on the salt-loading rat2007

    • Author(s)
      M Yoshika, Y Komiyama, H Takahashi
    • Organizer
      The 30^<th> Japanese congress for hyper- tension
    • Place of Presentation
      Naha
    • Year and Date
      20071000
    • Description
      「研究成果報告書概要(欧文)」より

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Published: 2010-06-09  

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