2007 Fiscal Year Final Research Report Summary
Development of a sensitive method for chimerism analysis using insertion-delection polymorphism
| Project/Area Number |
18590547
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Laboratory medicine
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| Research Institution | Clinical research Center, Nagoya National Hospital. |
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Principal Investigator |
OHASHI Haruhiko Clinical research Center, Nagoya National Hospital., National Hospital Organization Nagoya Medical Center, Clinical Research Center, Department Head (20360810)
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| Project Period (FY) |
2006 – 2007
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| Keywords | chimerism analysis / hematopoietic stem cell transplantation / genomic polymorphism |
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| Research Abstract |
The aim of this project is to establish a novel method of chimerism analysis, which is to determine the ratios of donor-derived hematopoietic cells after allogeneic hematopoietic stem cell transplantation(CST) , with insertion-deletion polymorphisms(indels) as genetic markers. We first intended to establish a real-time PCR-based method, however, because the running costs for this method as a routine clinical examination turned out to be too high, we tried to establish fluorescence-labeled PCR-based method(the indels-PCR method).
We first chose 8 indels markers(insertion sized 21-40 base pairs) , which were reported to be highly polymorphic in the Japanese population from a database, and assessed the utility for chimerism analysis DNA of peripheral blood cells from 71 CST donor/recipient pairs was analyzed, and the selected 8 markers could discriminate genetic differences in 67 pairs. With the addition of the X- and Y-chromosome-linked amelogenin marker, chimerism analysis could be performed for all the 71 pairs. Thus, chiraerism analysisis possible with a lelatively small number of indels markers.
We next compared the indels-PCR method to conventional short tandem repeat-based method(the STR-PCR method) . The two recognized drawbacks of the STR-PCR method are 1) stuttered peak formation that could obscure the distinction of alleles specific for the donor and the recipient, and 2) the inconsistency of amplification efficiency between alleles of different sizes that makes the calculation of donor-derived cell ratios difficult. The indels-PCR method was free from the former problem, and for the latter problem it showed higher consistency compared to the STR-PCR method. The indels-PCR method is more accurate to assess the ratios of donor-derived cells after CST compared to the conventional STR-PCR method, and it could be a practically useful method for chimerism analysis.
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[Journal Article] Feasibility and Results of Bone Marrow Transplantation from an HLA-Mismatched Unrelated Donor for Children and Young Adults with Acquired Severe Aplastic Anemia.2007
Author(s)
Yagasaki H, Takahashi Y, Kudo K, Ohashi H, Mama A, Yamamoto T, Tanaka M, Yoshida N, Hidaka H, Nishio N, Kojima S
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Journal Title
International Journal of Hematology 85
Pages: 437-42
Description
「研究成果報告書概要(和文)」より
Peer Reviewed
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[Journal Article] Successful nonmyeloablative bone marrow transplantation for leukocyte adhesion deficiency type I from an unrelated donor.2007
Author(s)
Tokunaga M, Miyamura K, Ohashi H, Ishiwada N, Terakura S, Ikeguchi M, Kuwatsuka Y, Inamoto Y, Oba T, Tsuchiya S, Kodera Y
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Journal Title
International Journal of Hematology 86
Pages: 91-95
Description
「研究成果報告書概要(和文)」より
Peer Reviewed
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[Journal Article] Feasibility and Results of Bone Marrow Transplantation from an HLA-Mismatched Unrelated Donor for Children and Young Adults with Acquired Severe Aplastic Anemia.2007
Author(s)
Yagasaki H, Takahashi Y, Kudo K, Ohashi H, Mama A, Yamamoto T, Tanaka M, Yoshida N, Hidaka H, Nishio N, Kojima S
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Journal Title
Int J Hematol 85
Pages: 437-442
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] A case of myelofibrosis with myeloid metaplasia with JAK2(V617F) mutation who developed fibrous tumors in multiple organs.2006
Author(s)
Tabuchi T, Ohashi H, Fukami S, Susaki N, Kunishima S, Yokozawa T, Nagai H, Moritani S, Ichihara S, Saito H, Hamaguchi M
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Journal Title
European Journal of Hematology 266
Pages: 264-266
Description
「研究成果報告書概要(和文)」より
Peer Reviewed
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[Journal Article] Loss of 06-methylguanine-DNA methyltransferase protein expression is a favorable prognostic marker in diffuse large B-cell lymphoma.2006
Author(s)
Ohno T, Hiraga I, Ohashi H, Sugisaki C, Li E, Asano H, Ito T, Nagai H, Yamashita Y, Mori N, Kinoshita T, Naoe T
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Journal Title
International Journal of Hematology 83
Pages: 1547-1553
Description
「研究成果報告書概要(和文)」より
Peer Reviewed
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[Journal Article] A case of myelofibrosis with myeloid metaplasia with JAK2(V617F) mutation who developed fibrous tumors in multiple organs.2006
Author(s)
Tabuchi T, Ohashi H, Fukami S, Susaki N, Kunishima S, Yokozawa T, Nagai H, Moritani S, Ichihara S, Saito H, Hamaguchi M
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Journal Title
Eur J Hematol 266
Pages: 264-266
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Loss of 06-methylguanine-DNA methyltransferase protein expression is a favorable prognostic marker in diffuse larg B-cell lymphoma.2006
Author(s)
Ohno T, Hiraga I, Ohashi H, Sugisaki C, Li E, Asano H, Ito T, Nagai H, Yamashita Y, Mori N, Kinoshita T, Naoe T
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Journal Title
Int J Hematol 83
Pages: 1547-1533
Description
「研究成果報告書概要(欧文)」より
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[Presentation] Hematopoietic cell Transplantation and detection of MRD for CBF leukemia2007
Author(s)
Kuwatsuka Y, Imabashi N, Nishiwaki S, Yanagisawa M, Yasuda R, Takahashi T, Oba T, Miyamura K, Ohashi H, Ito M, Kodera Y
Organizer
The 29th Annual Meeting of the Japanese Society of Hematopoietic Cell Transplantation
Place of Presentation
Fukuoka
Year and Date
2007-02-16
Description
「研究成果報告書概要(欧文)」より
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