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2007 Fiscal Year Final Research Report Summary

Analyses of polymorphisms in the FUT2 and FUT3 and mechanism of synthesis of Lewis blood group antigens

Research Project

Project/Area Number 18590647
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Legal medicine
Research InstitutionKurume University

Principal Investigator

SOEJIMA Mikiko  Kurume University, Forensic Medicine and Human Genetics, Assistant Professor (80279140)

Co-Investigator(Kenkyū-buntansha) KODA Yoshiro  Kurume University, Forensic Medicine and Human Genetics, Professor (90231307)
Project Period (FY) 2006 – 2007
KeywordsFUT2 / FUT3 / Lewis blood groups / glycosyltransferase
Research Abstract

We identified 24 SNPs containing seven novel SNPs (three of them are inactivating mutations) in the coding region of the FUT2 in Ovambos, Turks, and Mongolian populations. The genetic variations observed in these populations were similar to that of neighboring ones and seemed to reflect the geographic background and history of human migration. During screening of the se^<fus>, a Sec1-FUT2-Sec1 hybrid allele was identified in a Mongolian sample. This result implies that we need to be careful in choosing PCR primers otherwise make false-positive typing of se^<fus> because of recombinant alleles, while this allele is rare. By analysis the promoter region of the FUT2, we found African-specific SNPs with high incidence that affected transcriptional activity in culture cells. It is possible that long linkage disequilibrium (LD) block containing this region in African populations and the upstream region is also under balancing selection as an independent block of coding region.
To elucidate the location of the Lewis and Se enzymes that regulate the synthesis of Lewis antigens in Golgi apparatus, we constructed chimeric genes, FUT23 and FUT32 that exchanged the N-terminal cytoplasmic regions of the FUT2 and FUT3. We observed higher expression of the Le^a than that of Le^b antigens on the COS-7 transfectants of FUT23 and FUT32 comparing to which of FUT2 and FUT3. This observation suggests that the localization of two enzymes is important for the expression of the Lewis antigens and the cytoplasmic domains contain the signal of that. We are under investigation of subcellular localization of the enzymes encoded by the fusion proteins with fluorescent proteins and the variations of the coding region of the FUT3 in several human populations.

  • Research Products

    (35 results)

All 2008 2007 2006 Other 0

All Journal Article (17 results) (of which Peer Reviewed: 7 results) Presentation (16 results) Book (1 results) Remarks (1 results)

  • [Journal Article] Secl-FUT2-Sec1 hybrid allele generated by interlocus gene conversion2008

    • Author(s)
      Soejima M, Fujihara J, Takeshita H, Koda Y
    • Journal Title

      Transfusion 48

      Pages: 488-492

    • Description
      「研究成果報告書概要(和文)」より
    • Peer Reviewed
  • [Journal Article] Distinct single nucleotide polymorphism pattern at the FUT2 promoter a mong human populations2008

    • Author(s)
      Soejima M, Koda Y
    • Journal Title

      Annals of Hematology 87

      Pages: 19-25

    • Description
      「研究成果報告書概要(和文)」より
    • Peer Reviewed
  • [Journal Article] Extremely high prevalence of DNASE1*1 allele in African populations2008

    • Author(s)
      Takeshita H, Fujihara J, Soejima M, Koda Y, Yasuda T, Nakajima T
    • Journal Title

      Cell Biochemistry and Function 26

      Pages: 151-153

    • Description
      「研究成果報告書概要(和文)」より
    • Peer Reviewed
  • [Journal Article] Sec1-FUT2-Sec1 hybrid allele generated by interlocus gene conversion.2008

    • Author(s)
      Soejima M, Fujihara J, Takeshita H, Koda Y
    • Journal Title

      Transfusion 48

      Pages: 488-492

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Distinct single nucleotide polymorphism pattern at the FUT2 promoter among human populations.2008

    • Author(s)
      Soejima M, Koda Y
    • Journal Title

      Annals of Hematology 87

      Pages: 19-25

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Extremely high prevalence of DNASEJ *1 allele in African populations.2008

    • Author(s)
      Takeshita H, Fujihara J, Soejima M, Koda Y, Yasuda T, Nakajima T
    • Journal Title

      Cell Biochemistry and Function 26

      Pages: 151-153

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] The distribution of haptoglobin-gene deletion(Hp^<del>)is restricted to East Asians2007

    • Author(s)
      Soejima M, Koda Y, Fujihara J, Takeshita H
    • Journal Title

      Transfusion 47

      Pages: 1948-1950

    • Description
      「研究成果報告書概要(和文)」より
    • Peer Reviewed
  • [Journal Article] Genetic variation of FUT2 in a Ghanaian population:identification of four novel mutations and inference and inference of balancing selection2007

    • Author(s)
      Soejima M, Pang H, Koda Y
    • Journal Title

      Annals of Hematology 86

      Pages: 199-204

    • Description
      「研究成果報告書概要(和文)」より
    • Peer Reviewed
  • [Journal Article] Population differences of two coding SNPs in pigmentation-related genes SLC24A5 and SLC45A22007

    • Author(s)
      Soejima M, Koda Y
    • Journal Title

      International Journal of Legal Medicine 121

      Pages: 36-39

    • Description
      「研究成果報告書概要(和文)」より
    • Peer Reviewed
  • [Journal Article] DNA polymorphismJournals(All articles are accepted after peer reviews)2007

    • Author(s)
      Soejima M, Koda Y
    • Journal Title

      vol.15

      Pages: 5

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] The distribution of haptoglobin-gene deletion(Hpdel) is restricted to East Asians.2007

    • Author(s)
      Soejima M, Koda Y, Fujihara J, Takeshita H
    • Journal Title

      Transfusion 47

      Pages: 1948-1950

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Genetic variatinn of FUT2 in a Ghanaian population : identification of four novel mutations and inference of balancing selection.2007

    • Author(s)
      Soejima M, Pang H, Koda Y
    • Journal Title

      Annals of Hematology 86

      Pages: 199-204

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Expression of Mina53,a product of a Myc target gene in mouse testis2006

    • Author(s)
      Tsuneoka M, Nishimune Y, Ohta K, Teye K, Tanaka H, Soejima M, Iida H, Inokuchi T, Kimura H, Koda Y.
    • Journal Title

      International Journal of Andrology 29

      Pages: 323-330

    • Description
      「研究成果報告書概要(和文)」より
    • Peer Reviewed
  • [Journal Article] DNA polymorphism

    • Author(s)
      Soejima M, Koda Y
    • Journal Title

      vol.16(in press)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Reply to Role of non-B DNA conformations in initiating non-allelic homologous recombination-derived Se^<fus> allele and the interlocus gene conversion-derived Sec1-FUT2-Sec1 hybrid allele.

    • Author(s)
      Soejima M, Koda Y
    • Journal Title

      Transfusion (in press)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Koda Y Rapid detection of Hp directly from diluted blood samples using real-time PCR.

    • Author(s)
      Soejima M
    • Journal Title

      Clinical Chemistry (in press)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Genetic variation of FUT2 in Ovambos, Turks, and Mongolians.

    • Author(s)
      Soejima M, Nakajima T, Fujihara J, Takeshita H, Koda Y
    • Journal Title

      Transfusion (in press)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] Polymorphisms and functional analysis ofcalcium channel TRPV6.2008

    • Author(s)
      Soejima M, Uezono Y, Sudo Y, Matsuo K, Nishioka T, Kamimura S, Koda Y
    • Organizer
      91st meeting of Japanese society of Legal Medicine
    • Place of Presentation
      Akita View Hotel
    • Year and Date
      2008-05-18
    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] 本態性高血圧症疾患に関連するDNA多型の同定と解析2007

    • Author(s)
      近江 俊徳, 副島 美貴子, 神田 芳郎, 岩本 禎彦
    • Organizer
      日本DNA多型学会
    • Place of Presentation
      大阪産業創造館
    • Year and Date
      2007-11-16
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] Identification and analysis of DNA polymorphisms associated with essential hypertension diseases.2007

    • Author(s)
      Omi T, Soejima M, Koda Y, Iwamoto S
    • Organizer
      16th meeting of Japanese society of DNA Polymorphism
    • Place of Presentation
      Osaka industrial creation center
    • Year and Date
      2007-11-16
    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] Sec1-FUT2-Sec1ハイブリッドアリルの同定2007

    • Author(s)
      副島 美貴子, 藤原 純子, 竹下 治男, 神田 芳郎
    • Organizer
      日本DNA多型学会第16回学術集会
    • Place of Presentation
      大阪産業創造館
    • Year and Date
      2007-11-15
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] Identification of Sec1-FUT2-Sec1 allele.2007

    • Author(s)
      Soejima M, Fujihara J, Takeshita H, Koda Y
    • Organizer
      16th meeting of Japanese society of DNA
    • Place of Presentation
      Osaka industrial creation center
    • Year and Date
      2007-11-15
    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] アジア人集団におけるハプログロビン欠失アリル(Hp^<del>)の分布2007

    • Author(s)
      副島 美貴子, 藤原 純子, 竹下 治男, 西岡 朋生, 上村 繁雄, 神田 芳郎
    • Organizer
      第57回日本法医学会九州地方会
    • Place of Presentation
      福岡大学
    • Year and Date
      2007-10-16
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] Distribution of Hp^<del> allele in Asian populations.2007

    • Author(s)
      Soejima M, Fujihara J, Takeshita H, Kamimura S, Koda Y
    • Organizer
      Japanese society of Legal Medicine, 57th Kyushu Regional congress
    • Place of Presentation
      Fukuoka University
    • Year and Date
      2007-10-16
    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] Genetic variation of the FUT2 and the FUT3 in two populations in Sri Lanka2007

    • Author(s)
      Koda Y, Soejima M.
    • Organizer
      The 9th INPALMS Congress on Legal Medicine and Forensic Sciences
    • Place of Presentation
      Colombo,Sri Lanka
    • Year and Date
      2007-07-24
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] カルシウムチャネルTRPV6の多様性と機能解析2007

    • Author(s)
      副島 美貴子, 上園 保仁, 須藤 結香, 松尾 清隆, 西岡 朋生, 上村 繁雄, 神田 芳郎
    • Organizer
      第91次日本法医学会総会
    • Place of Presentation
      秋田ビューホテル
    • Year and Date
      2007-05-18
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] FOXP2遺伝子を用いた人獣鑑別法について2006

    • Author(s)
      廣重 憲一, 副島 美貴子, 西岡 朋生, 上村 繁雄, 神田 芳郎
    • Organizer
      第56回日本法医学会九州地方会
    • Place of Presentation
      サザンプラザ海邦
    • Year and Date
      2006-11-17
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] FUT2プロモーター領域の解析2006

    • Author(s)
      副島 美貴子, 神田 芳郎
    • Organizer
      日本DNA多型学会第15回学術集会
    • Place of Presentation
      福山県民文化センターふくやま
    • Year and Date
      2006-11-16
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] Analysis of promoter region of the FUT2.2006

    • Author(s)
      Soejima M, Koda Y
    • Organizer
      15th meeting of Japanese society of DNA
    • Place of Presentation
      Fukuyama prefectural culture center Fukuyama
    • Year and Date
      2006-11-16
    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] DNA多型を用いた帰属集団推定を目的とした変性高速液体クロマトグラフィ-法の検討2006

    • Author(s)
      副島 美貴子, 上村 繁雄, 神田 芳郎
    • Organizer
      第90次日本法医学会総会
    • Place of Presentation
      福岡国際会議場
    • Year and Date
      2006-04-27
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] Investigation of DHPLC as a tool of estimation of ancestry by using Ancestry Informative Markers(AIMs).2006

    • Author(s)
      Soejima M, Kamimura S, Koda Y
    • Organizer
      91sT meeting of Japanese society of Legal Medicine
    • Place of Presentation
      Fukuoka international congress center
    • Year and Date
      2006-04-27
    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] Genetic variation of the FUT2 and the FUT3 in two populations in Sri Lanka.

    • Author(s)
      Koda Y, Soejima M.
    • Organizer
      The 9th INPALMS Congress on Legal Medicine and Forensic Sciences
    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] Method of human identification using FOXP2.0

    • Author(s)
      Hiroshige K, Soejima M, Nishioka T, Kamimura S, Koda Y
    • Organizer
      Japanese society of Legal Medicine, 56th Kyushu Regional congress
    • Place of Presentation
      Southern plaza Kaiho
    • Year and Date
      00001117
    • Description
      「研究成果報告書概要(欧文)」より
  • [Book] DNA多型Vol.152006

    • Author(s)
      副島 美貴子, 神田 芳郎
    • Total Pages
      5
    • Publisher
      東洋書店
    • Description
      「研究成果報告書概要(和文)」より
  • [Remarks] 「研究成果報告書概要(和文)」より

    • URL

      http://www.med.kurume-u.ac.jp/med/foren/

URL: 

Published: 2010-02-04  

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