The investigation of gastric mucosal epithelial cell proliferative factor in Helicobacter pylori

2007 Fiscal Year Final Research Report Summary

• Project/Area Number: 18590673
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Gastroenterology
• Research Institution: University of Fukui
• Principal Investigator: YAMAZAKI Yukinao University of Fukui, University of Fukui Hospital, Associate Professor (10293416)
• Co-Investigator(Kenkyū-buntansha): SUTO Hiroyuki University of Fukui, Hospital, Assistant Professor (50345683) ; ITO Yoshiyuki University of Fukui, Hospital, Lecturer (60313748)
• Project Period (FY): 2006 – 2007
• Keywords: H-pylori / caz PAI / Proliferative Factor

Research Abstract

[Aim]In this research, we focused the pathogenic factor CagA, encoded in cag pathogenicity island (cag PAI) of Helicobacter pylori (H. pylori) and CagY, one of the proteins composed the type IV secretion system. The aim of this study was to investigate the gastric mucosal epithelial cell proliferative factor in H. pylori using the wild type, cagA knock-out mutant (ΔcagA) and cagY knock-out one (ΔcagY).
[Plans] 1. The comparative evaluation of the changes of AGS cell form by the wild type, ΔcagA and ΔcagY infections.
2. The evaluation of the molecular weight of CagA, in the wild type, ΔcagA and ΔcagY using the immunohistochemistry.
3. The evaluation of the intracellular existence and the location of CagY using the vector with the fluorescence method.
[Results] AGS cells infected ΔcagY did not show the hummingbird phenomenon, which was markedly demonstrated at the ones infected the wild type. However, AGS cells infected ΔcagA mildly showed the hummingbird phenomenon. It seemed that CagY play an important role to induce the hummingbird phenomenon at the cells infected H. pylori. Moreover, the immunohistochemistry to the CagA protein existed in the H. pylori demonstrated that the molecular weight of CagA in the ΔcagY was different from that in the wild type and ΔcagA. It suggested that the molecular weight of CagA depended on the CagY protein. These results suggested that CagY, interacted with pathogenic factor CagA might be important factor in relation to the pathogenicity of H. pylori. On the other hand, to evaluate the intracellular existence and the location of CagY using the vector with fluorescence method was failed regrettably.
[Conclusion] It seemed that CagY, interacted with pathogenic factor CagA, was likely to play an important role in relation to the pathogenicity of H. pylori.

Research Products

• [Journal Article] H. pylori除菌後の長期経過による上腹部症状の変化,「特集Helicobacter pylori感染と機能性dyspepsia(FD)」 (2008)
  • Author(s): 山崎幸直, 吉田郁美, 松田秀岳, 大野 崇, 真崎竜邦, 里見聡子, 大谷昌弘, 李 相植, 須藤弘之, 伊藤義幸, 東 健
  • Journal Title: Journal of Helicobacter Research 12(3) Pages: 195-199
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] The long-term effect of Helicobacter pylori eradication therapy on dyspepsia symptoms (2008)
  • Author(s): Yamazaki, Y., Yoshida, I., Matsuda, H., Ohno, T., Masaki, R., Satomi, S., Ohtani, M., Lee, S., Suto, H., Ito, Y., Azuma, T
  • Journal Title: Journal of Helicobacter Research 12(3) Pages: 195-199
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Relationship between the diversity of the cagA gene of Helicobacter pylori and gastric cancer in Okinawa, Japan. (2006)
  • Author(s): Satomi S., Yamakawa A., Matsunaga S., Masaki R., Inagaki T., Okuda T., Suto H., Ito Y., Yamazaki Y., Kuriyama M., Keida Y., Kutsumi H., Azuma T
  • Journal Title: Journal of Gastroenterology 41(7) Pages: 668-673
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Relationship between the diversity of the cagA gene of Helicobacter pylori and gastric cancer in Okinawa, Japan (2006)
  • Author(s): Satomi S., Yamakawa A., Matsunaga S., Masaki R., Inagaki T., Okuda T., Suto H., Ito Y., Yamazaki Y., Kuriyama M., Keida Y., Kutsumi H., Azuma, T
  • Journal Title: Journal of Gastroenterology 41(7) Pages: 668-673
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Low Serum Pepsinogen I/II Ratio is a Risk Factor of Obesity and Gastroesophageal Reflux Disease after Cure of Helicobacter pylori Infection. (2007)
  • Author(s): Suto H., Ohno T., Matsunaga S., Masaki R., Inagaki T., Ohtani M., Lee S., Ito Y., Yamazaki Y., Kuriyama M., Kato T.
  • Organizer: Digestive Disease Week 2007
  • Place of Presentation: Washington, DC, USA
  • Year and Date: 2007-05-22
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Low Serum Pepsinogen I/II Ratio is a Risk Factor of Obesity and Gastroesophageal Reflux Disease after Cure of Helicobacter pylori Infection (2007)
  • Author(s): Suto, H., Ohno, T., Matsunaga, S., Masaki, R., Inagaki, T., Ohtani, M., Lee, S., Ito, Y., Yamazaki, Y., Kuriyama, M., Kato, T
  • Organizer: Digestive Disease Week 2007
  • Place of Presentation: Washington, DC, USA
  • Year and Date: 2007-05-22
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Novel Diversity Site of Helicobacter pylori VacA is Critical for Cytotoxin Activity and Clinical Outcome. (2007)
  • Author(s): Inagaki T., Ito Y., Ohno T., Matsunaga S., Masaki R., Ohtani M., Lee S., Suto H., Kuriyama M., Keida Y., Azuma T., Wada A., Hirayama T., Yamazaki Y.
  • Organizer: Digestive Disease Week 2007
  • Place of Presentation: Washington, DC, USA
  • Year and Date: 2007-05-20
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Novel Diversity Site of Helicobacter pylori VacA is Critical for Cytotoxin Activity and Clinical Outcome (2007)
  • Author(s): Inagaki, T., Ito, Y., Matsunaga, S., Masaki, R., Ohtani, M., Lee, S., Suto, H., Kuriyama, M., Keida, Y., Azuma, T., Wada, A., Hirayama, T., Yamazaki, Y
  • Organizer: Digestive Disease Week 2007
  • Place of Presentation: Washington, DC, USA
  • Year and Date: 2007-05-20
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Low serum pepsinogen I/II ratio induces an increase in body mass index after eradication of H. pylori. (2006)
  • Author(s): Suto H., Matsunaga S., Masaki R., Inagaki T., Satomi S., Okuda T., Muramatsu A., Lee S., Ito Y., Yamazaki Y., Kuriyama M., Kato T., Azuma T.
  • Organizer: Digestive Disease Week 2007
  • Place of Presentation: Los Angeles, USA
  • Year and Date: 2006-05-23
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Low Serum Pepsinogen I/II Ratio Induces an Increase in Body Mass Index after Eradication of H pylori (2006)
  • Author(s): Suto, H., Matsunaga, S., Masaki, R., Inagaki, T., Satomi, S., Okuda, T., Muramatsu, A., Lee, S., Ito, Y., Yamazaki, Y., Kuriyama, M., Kato, T., Azuma, T
  • Organizer: Digestive Disease Week 2006
  • Place of Presentation: Los Angeles, USA
  • Year and Date: 2006-05-23
  • Description: 「研究成果報告書概要(欧文)」より