2007 Fiscal Year Final Research Report Summary
Apoptotic pathway in the rat small intestinal mucosa is different between physiological conditions and ischemia-reperfusion
| Project/Area Number |
18590690
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Gastroenterology
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| Research Institution | Saga University |
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Principal Investigator |
FUJIMOTO Kazuma Saga University, Saga Medical School Dept. of Gastrointestinal Endoscopy, Professor (50181392)
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| Project Period (FY) |
2006 – 2007
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| Keywords | central nervous system / ileum / jejunum / metabolism / appetite / absorption / digestion / ischemia-reperfusion |
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| Research Abstract |
We have previously demonstrated that fasting and ischemia-reperfusion (I/R) induced apoptosis in rat intestinal mucosa. It is widely accepted that apoptosis is induced through two main pathways. This study aimed to compare apoptotic pathways following physiological conditions including fasting and I/R. Rats were divided into two groups: the I/R group involved occlusion of the superior mesenteric artery for 60 min, followed by 60 min reperfusion; while the fasting group involved fasting for 24 or 48 h. Additional rats were infused several physiological substances into the cerebro-ventricle. Intestinal apoptosis was assessed as% fragmented DNA, by electrophoresis, and by a TUNEL assay. Apoptotic proteins including death ligands/receptors, caspases were evaluated by Western blot analysis. Small intestinal mucosal height and mitochondrial dehydrogenase function were assessed. Physiological manipulation and I/R significantly induced intestinal apoptosis. Mucosal height was significantly decreased in fasting rats, and mitochondrial dysfunction was induced only by I/R. Expressions of Fas, FasL and TNFR1 were enhanced in rats of both groups. After I/R, expressions of cytochrome c and cleaved caspase-9 were significantly increased. In contrast, expressions of cleaved caspase-8 and cleaved caspase-3 increased in the physiological conditions. Physiological conditions promoted mucosal apoptosis via receptor-mediated type I apoptotic pathway in the rat small intestine, and I/R induced apoptosis via mitochondria-mediated type II pathway.
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[Journal Article] Fujimoto Suppression of intestinal mucosal apoptosis by ghrelin in fasted rats2008
Author(s)
Park, JM., Kakimoto, T., Kuroki, T., Shiraishi, R., Fujise, T., Iwakiri R., Fujimoto, K
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Journal Title
Exp Biol Med 233
Pages: 48-56
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Fujimoto Indigestible material attenuated changes in apoptosis in the fasted rat jejunal mucosa2008
Author(s)
Kakimoto, T., Fujise, T., Shiraishi, R., Kuroki, T., Park, JM., Ootani, A., Sakata, Y., Tsunada, S., Iwakiri, R., Fujimoto K
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Journal Title
Exp Biol Med 233
Pages: 310-316
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Adipocytes and preadipocytes promote the proliferation of colon cancer cells in vitro2007
Author(s)
Amemori, S., Ootani, A., Aoki, S., Fujise, T., Shimoda, R., Kakimoto, T., Shiraishi, R., Sakata, Y., Tsunada, S., Iwakiri, R., Fujimoto, K
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Journal Title
Am. J. Physiol 292
Pages: G923-G929
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Long term feeding of various fat diets modulates azoxymethane-induced colon carcinogenesis through Wnt/β-catenin signaling in rats2007
Author(s)
Fujise, T., Iwakiri, R., Kakimoto, T., Shiraishi, R., Sakata, Y., Wu, B., Tsunada, S., Ootani, A., Fujimoto, K
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Journal Title
Am J Physiol 292
Pages: G1150-1156
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Differentiation of Gastric Surface Mucous Cells(GSM06) Induced by Air Liquid Interface Is Regulated' Partly through Mitogen-Activated Protein Kinase Pathway2007
Author(s)
Yokoyama, F., Sakata, Y., Ootani, A., Fujise, T., Kakimoto, T., Amemori, S., Kuroki, T., Tsunada, S., Iwakiri, R., Fujimoto, K
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Journal Title
J. Gastroenterol. Hepatol 22
Pages: 2310-2315
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Apoptotic pathway in the rat small intestinal mucosa is different between fasting and ischemia-reperfusion2006
Author(s)
Fujise, T., Iwakiri, R., Wu, B., Amemori, S., Kakimoto, T., Yokoyama, F., Sakata, Y., Tsunada, S., Fujimoto, K
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Journal Title
Am. J. Physio 291
Pages: G110-G116
Description
「研究成果報告書概要(欧文)」より
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