2007 Fiscal Year Final Research Report Summary
Clonal diversity and its imphcution in multistage development of hepatocellnlar caronoma
| Project/Area Number |
18590729
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Gastroenterology
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| Research Institution | Kyoto University |
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Principal Investigator |
FUKUDA Yoshihiro Kyoto University, Graduate School of Mechicine, Professor (50127130)
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| Co-Investigator(Kenkyū-buntansha) |
UEDA Mikiko Kyoto University, Graduate School of Medicine, Lecturer (20322159)
NISHIMURA Takafumi Kyoto University, Graduate School of Medicine, Assistant Professor (40378732)
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| Project Period (FY) |
2006 – 2007
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| Keywords | hepatocellular carcinoma / living donor liver transplantation |
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| Research Abstract |
Genomic diversity and its implication in multistage development were explored by analyzing chrmosomal alterations found in multiple liver nodule obtained from patients with hepatocellular carcinoma who had and one living donor orthotopic liver transplantation.
Tumor and non tumor tissue were obtained at the time of surgery, from patients who had provided written informed consent DNA was extracted by using conventional proteinase K/phenol-chloroform method Four-hundred pane of PCR primers that distribute almost equally throughout 22 autosome and X chromosome wee used to amplify microsatellite loci. Allelic imbalances were determined on the bass of the area under the curve of PCR product that derived from the tumor and the non-tumor sample Single nucleotide polymorphism (SNP) array were used to discriminate chromosomal and losses.
Seventy-two patients with hepatocellular carcinoma had received living-donor liver transplantation in between April 2004 and August and August 2007. A total of 20 nodules were obtained from eight of these patients. Recurrent chromosome alterations, such as -1p, +1q, +5, +7, -8p, +8q, -16q, -17p, were detected. Chromosomal relationship analysis revealed that seven patients out of eight had metastatic nodule, while only one patient had tumors of multicentric origin.
Furthermore, chromosome analysis of 30 nodules m a patient detected both genetically stable tumors that was hind to have common chromosome alterations such as -1p, +1q, -6q, -16q, +22q, and unstable tumors that had multiple alterations throughout almost all chromosome.
Although chromosomal alterations detected m hepatocellular carcinoma m patients with end-stage liver disease were generally stable, chromosomal instability might occur by an unknown but possibly different mechanism.
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Research Products
(24 results)
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[Journal Article] Aberrant methylation of multiple tumor suppressor genes in aging liver, chronic hepatitis and hepatocellular carcinoma2008
Author(s)
Nishida, N, Nagasaka, T, Nishimura, T, Ikai, I, Boland, CR, Goel, A
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Journal Title
Hepatology 47
Pages: 908-18
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Expansion of selection criteria for patients with hepatocellular carcinoma in living donor liver transplantation.2007
Author(s)
Ito T, Takada Y, Ueda M, Haga H, Maetani Y, Oike F, Ogawa K, Sakamoto S, Ogura Y, Egawa H, Tanaka K, Uemoto S.
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Journal Title
Liver Transplantation 13
Pages: 1637-44
Description
「研究成果報告書概要(和文)」より
Peer Reviewed
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[Journal Article] Living donor liver transplantation for patients with HCC exceeding the Milan criteria: a proposal of expanded criteria.2007
Author(s)
Takada Y, Ito T, Ueda M, Saka moto S, Haga H, Maetani Y, Ogawa K, Ogura Y, Oike F, Egawa H, Uemoto S.
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Journal Title
Digestive Disease 25
Pages: 299-302
Description
「研究成果報告書概要(和文)」より
Peer Reviewed
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[Journal Article] Prevalence and incidence of anemia in Japanese cancer patients receiving outpatient chemotherapy.2007
Author(s)
Kitano T, Tada H, Nishimura T, Teramukai S, Kanai M, Nishimura T, Misawa A, Yoshikawa K, Yasuda H, Ishiguro H, Matsumoto S, Yanagihara K, Fukushima M.
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Journal Title
International Journal of Hematology 87
Pages: 37-41
Description
「研究成果報告書概要(和文)」より
Peer Reviewed
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[Journal Article] The technical pitfalls of duct-to-duct bihary reconstruction m pediatric living-donor left-lobe liver transplantation : The impact of stent placement2007
Author(s)
Sakamoto, S, Egaiva, H, Ogawa, K, Ogura, Y, Oike, F, Ueda, M, Yazumi, S, Shibata, T, Takada, Y, Uemoto, S
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Journal Title
Pediatric transplantation (Epub)
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Expansion of selection criteria for patients with hepatocellular carcinoma in living donor liver transplantation2007
Author(s)
Ito, T, Takada, Y, Ueda, M, Haga, H, Maetani, Y, Oike, F, Ogawa, K, Sakamoto, S, Ogura, Y, Egavva, H, Tanaka, K, Uemoto, S
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Journal Title
Liver Transplantation 13
Pages: 1637-44
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Living donor liver transplantation for patients with HCC exceeding the Milan criteria : a proposal of expanded criteria2007
Author(s)
Takada, Y, Ito, T, Ueda, M, Sakamoto, S, Haga, H, Maetani, Y, Ogawa, K Ogura, Y, Oike, F, Egawa, H, Uemoto, S
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Journal Title
Digestive Disease 25
Pages: 299-302
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Living donor liver transplantation with reduced monosegments for neonates and small infante2007
Author(s)
Ogawa, K, Kasahara, M, Sakamoto, S, Ito, T, Taira, K Oike, F, Ueda, M, Egawa, H, Takada, Y, Uemoto, S
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Journal Title
Transplantation 83
Pages: 1337-40
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Prevalenceiand incidence of anemia in Japanesecancer patients receiving outpatient chemotherapy2007
Author(s)
Kitano, T, Tada, H, Nishimura, T, Teramukai, S, Kauai, M, Nishimura, T, Misawa, A, Yoshikawa, K, Yasuda, H, Ishiguro, H, Matsumoto, S, Yanagihara, K, Fukushima, M
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Journal Title
International Journal of Hematology 87
Pages: 37-41
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Extensive methylation is associated with beta-catenin mutations in hepatocellular carcinoma, evidence for two2007
Author(s)
Nishida, N, Nishimura, T, Nagasaka, T, Ikai, I, Goel, A, Boland, CR
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Journal Title
Cancer Res 67
Pages: 4586-94
Description
「研究成果報告書概要(欧文)」より
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