2007 Fiscal Year Final Research Report Summary
Search for novel genomic regions related to liver carcinogenesis by using a hepatitis B virus integration site as a genetic probe
| Project/Area Number |
18590742
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Gastroenterology
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| Research Institution | Kyoto Prefectural University of Medicine |
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Principal Investigator |
MINAMI Masahito Kyoto Prefectural University of Medicine, Graduate School of Medical Science, Research Associate (60326220)
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| Project Period (FY) |
2006 – 2007
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| Keywords | hepatitis B virus / integration / liver carcinogenesis |
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| Research Abstract |
Human genomic regions flanking integrated hepatitis B virus (HBV) were investigated from liver tissues of chronic hepatitis, liver cancers and non-cancerous tissues surrounding liver cancers. We developed an HBV-Alu PCR method to efficiently amplify the viral-host junctions. From 26 cases with chronic hepatitis and 10 with liver cancer, 75 viral-host junctions were cloned and genomic sequences were determined. Together with genomic database search and our results, we could identify a human telomerase reverse transcriptase (hTERT) gene and MLL4 gene as common integration sites. These two genes were found integrated exclusively in liver cancer tissues. HBV integration into an hTERT gene was found in about 5 % of liver cancer cases where HBV integration sites were investigated.
To investigate when the integration into hTERT occur during chronic HBV infection, we searched for HBV-hTERT chimeric sequences in chronic hepatitis tissues by PCR with several primer sets, but no HBV-hTERT chimeras were detected. Thus it is suggested that HBV integration into the hTERT gene occurs in more advanced stages of HBV infection than chronic hepatitis.
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Research Products
(25 results)
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[Journal Article] Low serum level of hepatitis B core-related antigen indicates unlikely reactivation of hepatitis after cessation of lamivudine therapy2007
Author(s)
Matsumoto A, Tanaka E, Minami M, Okanoue T, Yatsuhashi H, Nagaoka S, Suzuki F, Kobayashi M, Chayama K, Imamura M, Yotsuyanagi H, Nakaoka S, Maki N, Kawata S, Kumada H, Iino S, Kiyosawa K.
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Journal Title
Hepatology Research 37(8)
Pages: 661-666
Description
「研究成果報告書概要(和文)」より
Peer Reviewed
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[Journal Article] Low serum level of hepatitis B core-related antigen indicates unlikely reactivation of hepatitis after cessation of lamivudine therapy2007
Author(s)
Matsumoto, A., Tanaka, E., Minami, M., Okanoue, T., Yatsuhashi, H., Nagaoka, S., Suzuki, F., Kobayashi, M., Chayama, K., Imamura, M., Yotsuyanagi, H., Nakaoka, S., Maki, N., Kawata, S., Kumada, H., Iino, S., Kiyosawa, K
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Journal Title
Hepatology Research 37(8)
Pages: 661-666
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Measurement of hepatitis B virus core-related antigen is valuable for identifying patients who are at low risk of lamivudine resistance2006
Author(s)
Tanaka E, Matsumoto A, Suzuki F, Kobayashi M, Mizokami M, Tanaka Y, Okanoue T, Minami M, Chayama K, Imamura M, Yatsuhashi H, Nagaoka S, Ybtsuyanagi H, Kawata S, Kimura T, Maki N, Iino S, Kiyosawa K; HBV Core-Related Antigen Study Group
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Journal Title
Liver International 26(1)
Pages: 90-96
Description
「研究成果報告書概要(和文)」より
Peer Reviewed
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[Journal Article] Prediction of breakthrough hepatitis due to lamivudine-resistant hepatitis B virus by a sensitive semiquantitative assay using peptide nucleic acids2006
Author(s)
Mori K, Minami M, Kirishima T, Kunimoto K, Okita M, Nakayama M, Makiyama A, Yamaoka J, Nakajima T, Yasui K, Itoh Y, Okanoue T.
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Journal Title
Intervirology 49(5)
Pages: 274-280
Description
「研究成果報告書概要(和文)」より
Peer Reviewed
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[Journal Article] HBV Core-Related Antigen Study Group. Measurement of hepatitis B virus core-related antigen is valuable for identifying patients who are at low risk of lamivudine resistance2006
Author(s)
Tanaka, E, Matsumoto, A, Suzuki, F, Kobayashi, M, Mizokami, M, Tanaka, Y, Okanoue, T, Minami, M, Chayama, K, Imamura, M, Yatsuhashi, H, Nagaoka, S, Yotsuyanagi, H, Kawata, S, Kimura, T, Maki, N, Iino, S, Kiyosawa, K
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Journal Title
Liver International 26(1)
Pages: 90-96
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Prediction of breakthrough hepatitis due to lamivudine-resistant hepatitis B virus by a sensitive semiquantitative assay using peptide nucleic acids2006
Author(s)
Mori, K., Minami, M., Kirishima, T., Kunimoto, K., Okita, M., Nakayama, M., Makiyama, A., Yamaoka, J., Nakajima, T., Yasui, K., Itoh, Y., Okanoue, T
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Journal Title
Intervirology 49(5)
Pages: 274-280
Description
「研究成果報告書概要(欧文)」より
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[Presentation] Identification of cancer-related genes using HBV integration as a sequence tag2006
Author(s)
Minami, M., Fujii, K., Miyagawa, M., Itoh, Y., Okanoue, T
Organizer
Annual Meeting of the American Association for the Study of Liver Disease (AASLD)
Place of Presentation
Boston, USA
Year and Date
20061027-20061031
Description
「研究成果報告書概要(欧文)」より
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[Presentation] 悪性リンパ腫の治療後に発症したHBs抗原陰性,HBVDNA陽性の急性肝炎2006
Author(s)
宮川 昌巳, 南 祐仁, 橋本 宏明, 森康 二郎, 藤井 恒太, 伊藤 義人, 清水 大介, 松本 洋典, 谷脇 雅史, 岡上 武
Organizer
第42回日本肝臓学会総会
Place of Presentation
京都
Year and Date
2006-05-25
Description
「研究成果報告書概要(和文)」より
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