2007 Fiscal Year Final Research Report Summary
Development of drug deliumy system for targeting to injured myocardium by a novel glycoside-binding protein
| Project/Area Number |
18590764
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Circulatory organs internal medicine
|
|---|
| Research Institution | Shinshu University |
|---|
Principal Investigator |
ISE Hirohiko Shinshu University, Division of Cardiovascular Sciences, Department of Organ Regeneration, Assistant Professor (10324253)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
TAKAHASHI Masafumi Division of Cardiovascular Sciences, Department of Organ Regeneration, Associate Professor (40296108)
IKEDA Uichi Division of Cardiovascular Sciences, Department of Organ Regeneration, Professor (30221063)
|
|---|
| Project Period (FY) |
2006 – 2007
|
| Keywords | Drug deliverv system / lectin / vimentin / desmin |
|---|
| Research Abstract |
We investigated a novel lectin that cardiomyocytes and vascular smooth muscle cells (VSMCs) express on cell surface, in order to elucidate the role of carbohydrate-modifications for regeneration and inflammation in cardiovascular diseases and to develop a drug delivery system by using a lectin. We examined the interaction of various glycoside chains with cardiomyocytes and VSMCs and cardiomyocytes by using glycoside-binding assay with various glycoside-bearing polymers-coated dishes. Cardiomyocytes and VSMCs had the interaction with N-acetylglucosamine (GIcNAc) specifically. Moreover, cardiomyocytes and VSMCs took up the GIcNAc-conjugated liposomes. From these results, we assumed that these cells have a GIcNAc-binding lectin on cell surface and tried to identify the lectin. A GIcNAc-recognizing lectin was identified as desmin and vimentin in cardiomyocytes and as vimentin in VSMCs by using two-dimensional electrophoresis and western blotting with GIcNAc-bearing polymer. To confirm whether vimentin and desmin bind to GIcNAc, we examined a binding capacity of vimentin and desmin for GIcNAc by using BIACORE. Vimentin and desmin interacted with GIcNAc-bearing polymer highly and specifically, whereas not glucose- and galactose-bearing polymers. Moreover, to examine whether these protein express on cell surface. we performed double-immunostaining of these cells with anti-vimentin or desmin antibody and fluorescence-conjugated GIcNAc-bearing polymer. We observed that these antibodies and polymer stained on the same region of cell surface by using laser scanning confocal microscopy. From these results, we suggested that as a novel function of vimentin and desmin. these proteins have a binding capacity for GIcNAc and express on surface of cardiomyocytes and vascular smooth muscle cells.
|
-
-
-
-
-
[Journal Article] A novel sphingosine-l-phosphate receptor agonist KRP-203 attenuates rat autoimmune myocarditis.2007
Author(s)
Ogawa R, Takahashi M, Hirose S, Morimoto H, Ise H, Murakami T, Yasue T, Kuriyama K, Hongo M, Kobayashi E, Ikeda U
-
Journal Title
Biochem Biophys Res Commun 361
Pages: 621-628
Description
「研究成果報告書概要(和文)」より
Peer Reviewed
-
-
[Journal Article] Effective uptake of N-acetylglucosamine-conjugated liposomes by cardiomyocytes in vitro2007
Author(s)
Aso, S., Ise, H., (Correspondina author). Takahashi, M., Kobayashi, S., Morimoto,H., Izawa, A., Goto, M., Ikeda
-
Journal Title
J. Control Release 122
Pages: 189-198
Description
「研究成果報告書概要(欧文)」より
-
-
[Journal Article] Development of liver regenerative therapy using glycoside-modified bone marrow cells2006
Author(s)
Misawa, R., Ise, H., (Corresponding author). Takahashi, M., Morimoto, H., Kobayashi, E., Miyagawa, S., Ikeda, U
-
Journal Title
Biochem Biophys Res Commun 342
Pages: 434-440
Description
「研究成果報告書概要(欧文)」より
-
-
-
-
-
-
-
-
-
-
-
-
-
