2009 Fiscal Year Final Research Report
The role and therapeutic application of blood stem/progenitor cells in lung injury
| Project/Area Number |
18590870
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Respiratory organ internal medicine
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| Research Institution | Nippon Medical School |
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Principal Investigator |
ABE Sinji Nippon Medical School, 医学部, 講師 (20386163)
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| Project Period (FY) |
2006 – 2009
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| Keywords | 再生医学 / 造血幹細胞 / 肺障害 |
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| Research Abstract |
Regeneration of various tissues by hematopoietic stem/progenitor cells has been reported and expected to be therapeutic application in future. This study was aimed to clarify the effect of mobilized bone marrow stem/progenitor cells on experimental lung injuries using granurocyte colony stimulating factor (G-CSF) and erythropoietin. In mice, mobilization of marrow stem cells by G-CSF is amplified by splenectomy. C57/Bl mice were splenectomized and then injected bleomycin intravenously. In splenectomized mice, lung injuries were induced as well as control mice. We gave G-CSF and erythropoietin subcutaneously day 0-5 after bleomycin injection and attempted to mobilize marrow stem/progenitor cells into lung. On day 21 and 28, bronchial alveolar lavage fluid (BALF) and lung tissues were evaluated. In BALF, the proportion of neutrophils in experimental groups was significantly reduced compared to control ones. However there was not significant difference in the degree of lung injuries. In acute lung injury model using intra-trachial injection of bleomycin, there was not significant improvement of lung injury in experimental groups.
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