2007 Fiscal Year Final Research Report Summary
Proteomic a-myofibroblastnalysis of activated- and inactivated
| Project/Area Number |
18590873
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Respiratory organ internal medicine
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| Research Institution | Kinki University |
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Principal Investigator |
MUNAKATA Hiroshi Kinki University, Sch Med, Professor (90111294)
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| Co-Investigator(Kenkyū-buntansha) |
MATSUDA Toshirou Kinki University, Hospital, Lecturer (80411586)
HAYAKAWA Sumio Hokkaidou University, 遺伝子病制御研究所, Assist Prof (00368292)
YOSHIDA Koji Kinki University, Sch Med, Associate Professor (60230736)
SAITO Akio Kinki University, Sch Med, Lecturer (40153788)
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| Project Period (FY) |
2006 – 2007
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| Keywords | Proteome / Myofibroblast / Activation |
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| Research Abstract |
Previously we showed that the expression of integrin aω, β1, β3 in MRC-5 cells increased by TGF-β. Increased expression of fibronectin and focal adhesion kinase was also shown. Here, we show the results of proteomics analysis of activation and inactivation of myofibroblast cell line, MRC-5. Transforming growth factor-b stimulated MRC-5 cell extract was compared with that from non-stimulated cells. Several proteins differentially expressed between stimulated and non-stimulated cells including actin-bindin proteins and molecular chaperones.
As previously reported the culture supernatant of A-549 inactivates myofibroblast MRC-5. RNA was obtained from MRC-5 cells after inactivation by the conditioned medium of A-549 and the expressions were analyzed using low density arrays. Expression of some proteins concerned in cell protrusions was increased. The cells treated with forskolin were subjected to proteome analysis. Inceased expression of actin capping protein was observed.
These results, together with previous our findings, suggests that signal transduction mediated by interantions between extracellular matrix and integrins are important in activation and inactivation of myofibroblasts.
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[Journal Article] Effects of ethyl-esterization, chain-lengths, unsaturation degrees, and hyperthermia on carcinostatic effect of omega-hydroxylated fatty acids.2007
Author(s)
Kusumoto K, Kageyama K, Matsuda T, Tomura TT, Munakata H, Tanaka H, Yazama F, Miwa N.
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Journal Title
Exp Oncol. 29(2)
Pages: 106-10
Description
「研究成果報告書概要(欧文)」より
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