2007 Fiscal Year Final Research Report Summary
Analysis of biological properties of hepatoma-derived growth factor as a biological marker of lung injury
| Project/Area Number |
18590874
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Respiratory organ internal medicine
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| Research Institution | Clinical Research Center, National Hospital Organization, Kinki-Chuo Chest Medical Center |
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Principal Investigator |
HAYASHI Seiji Clinical Research Center, National Hospital Organization, Kinki-Chuo Chest Medical Center, NHO近畿中央胸部疾患センター, 副院長 (70218577)
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| Co-Investigator(Kenkyū-buntansha) |
OHTSUKA Atsushi National Hospital Organization Kinki-Chuo Chest Medical Center, 内科医師 (70426518)
INOUE Yoshikazu National Hospital Organization Kinki-Chuo Chest Medical Center, 研究部長 (90240895)
TACHIBANA Kazunobu National Hospital Organization Kinki-Chuo Chest Medical Center, 内科医長 (10311482)
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| Project Period (FY) |
2006 – 2007
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| Keywords | microsampling / cytokine / hepatoma-derived growth factor / airway-epithelial lining fluid / diffuse lung disease / interstitial pneumonia / multiplex suspension array system |
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| Research Abstract |
Airway-epithelial lining fluid (ELF) was obtained by using Olympus micro sampling probe BC-401C from 105 cases of various lung diseases (interstitial pneumonia, sarcoidosis, eosinophilic pneumonia and hemosputum patients without diffuse lung diseases). The analysis of samples of fifty-seven (27 cases of interstital pneumonia, 4 cases of sarcoidosis, 4 cases pf eosinophilic pneumonia, 4 cases of hemosputum and 18 case of other disases) has been completed, and total ELF amount was 30.2±15.3mg. The ELF was eluated by 1 ml of saline from three probes for each case. Total amount of protein in the ELF was 666.6±700.3microg/mL. We have reported that hepatoma-derived growth factor (HDGF) is relevant to the pathogenesis of inflammatory lung diseases such as sarcoidosis. HDGF was detected by ELISA system in all the samples tested, and the range of concentration of HDGF was from 6.7 to 73.5 ng/ml. Cytokine in samples from two interstital pulmonary fibrosis cases was assessed by using Multiplex Suspension Array System 27Plex (BioPlex). Sixteen cytokines such as IL-1b, -5, -6 were detected. Thus, it is confirmed safety and usefulness of the microsampling method, because any clinically significant adverse events were not observed, and sufficient amount of ELF for analysis was obtained by the method for each case.
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