2007 Fiscal Year Final Research Report Summary
Role of in glomerular injury
| Project/Area Number |
18590885
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Kidney internal medicine
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| Research Institution | Niigata University |
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Principal Investigator |
TAKEDA Tetsuro Niigata University, Hospital, Assistant professor (10361924)
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| Co-Investigator(Kenkyū-buntansha) |
SAITO Akihiko Niigata University, Graduate School of Medical and Dental Sciences, Professor (80293207)
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| Project Period (FY) |
2006 – 2007
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| Keywords | Renal glomerulus / Podocyte / protein-protein interaction / Sugar transferase / Angiotensin II / Honeycomb-patterned film / Altered glycation / Adaptor protein |
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| Research Abstract |
We have previously demonstrated that an anti-adhesin molecule, podocalyxin binds to the adaptor proteins and that the interaction is important to maintain the unique organization of podocyte structure. In this research project, we focused on altered glycation of podocalyxin that should affect glomerular adhesion, indicating a marker of severe kidney diseases. We investigated what kind of sugar transferases are specifically expressed in podocyte and carried out 2-D SDS-PAGE to detect the altered glycation of podocalyxin. Also, we tested podocyte-specific mRNA expression whether novel material of substratum, honeycomb patterned film provides suitable circumference for podocyte culture.
1. We found that two unique sugar transferases (glycoprotein-N-acetylgalactosamine-3-13-galactosyltransferase, C1Gal-t-1, UDP-G1cNAc 2-epimerase, GNE) are expressed in podocyte in vitro and in vivo by RT-PCR.
2. Podocyte-specific mRNA (nephrin, CD2AP, podocalyxin) expression was increased in podocyte cultured on honeycomb patterned-film. Multi-layered cells cultured on this film were observed by scanning electron microscopy.
3. We also found that the interaction between podocalyxin and adaptor proteins (NHERF2 and ezrin) in podocyte cell lines was reduced in proportion to the angiotensin II concentration.
4. We failed to analyze sugar chains of purified podocalyxin, because to technical problems.
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[Journal Article] Functional Characterization of a Novel Missense CLCN5 Mutation Causing Alterations in Proximal Tubular Endocytic Machinery in Dent's Disease2007
Author(s)
A, Tanuma, H, Sato, T, Takeda, M, Hosojima, H, Obayashi, H, Hama, N, Iino, K, Hosaka, R, Kaseda, N, Imai, M, Ueno, M, Yamazaki, K, Sakimura, F, Gejyo, A, Saito
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Journal Title
Nephron Physiol 107(4)
Pages: 87-97
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Megalin-mediated endocytosis of cystatin C in proximal tubule cells2007
Author(s)
R, Kaseda, N, Iino, M, Hosojima, T, Takeda, K, Hosaka, A, Kobayashi, K, Yamamoto, A, Suzuki, A, Kasai, Y, Suzuki, F, Gejyo, A, Saito
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Journal Title
Biochemical and Biophysical Research Communications 357(4)
Pages: 1130-1134
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Para-capillary electron-dense deposits reduce glomerular filtration in patients with primary glomerular diseases2006
Author(s)
S, Fukase, JJ, Kazama, H, Mori, S, Iguchi, T, Takeda, M, Ueno, S, Nishi, I, Narita, F, Gejyo
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Journal Title
Clinical and Experimental Nephrology 10(1)
Pages: 33-39
Description
「研究成果報告書概要(欧文)」より
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[Presentation] TJ Thekkumkara, Tetsuro Takeda, Fumitake Gejyo, Akihiko Saito: Angiotensin II and insulin-mediated signaling cross-talks for the expression of megalin in cultured proximal tubule cells2007
Author(s)
M, Hosojima, N, Iino, A, Kobayashi, R, Kaseda, A, Nishiyama
Organizer
The 40th Annual Meeting of the American Society of Nephrology/Renal Week 2007
Place of Presentation
San Francisco, CA, USA
Year and Date
20071031-1105
Description
「研究成果報告書概要(欧文)」より
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[Presentation] Long-term renal prognosis of Japanese patients with obesity/metabolic syndrome related nephropathy2006
Author(s)
A, Saito, M, Ueno, M, Hosojima, R, Kaseda, K, Hosaka, N, Iino, T, Takeda, S, Nishi, Y, Suzuki, F, Gejyo
Organizer
The 39th Annual Meeting of the American Society of Nephrology/Renal Week 2006
Place of Presentation
San Diego, CA, USA
Year and Date
20061114-19
Description
「研究成果報告書概要(欧文)」より
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