2007 Fiscal Year Final Research Report Summary
Clarification of the pathogenic mechanism of proteinuria
| Project/Area Number |
18590886
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Kidney internal medicine
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| Research Institution | Niigata University |
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Principal Investigator |
KAWACHI Hiroshi Niigata University, Institute of Medcine and Dentistry, Associate Professor (60242400)
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| Project Period (FY) |
2006 – 2007
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| Keywords | proteinuria / podocyte / slit diaphragm / SV2B / ephrin B1 / subtraction / siRNA |
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| Research Abstract |
We have been studying the nature of slit diaphragm (SD) of podocyte that is accepted to play a critical role for maintaining the barrier function of glomerular capillary wall. However, the molecular composition of the SD was not fully understood yet. In this project, we intended to identify the functional molecule of the SD. In the first year of this project (2006), to identify the molecules which are involved in the development of proteinuria, the subtraction assay was performed using normal and proteinuric glomerular materials. Synaptic vesicle-protein 2B (SV2B) is detected to be clearly downregulated in the proteinuric glomeruli. In the second year (2007), the expression and the function of SV2B in podocyte were analyzed. The analyses with siRNA techniques showed that SV2B play a role for maintaining the proper localization of CD2AP, a critical molecule of the SD. We also observed that the expression of ephrin B1 is clearly decreased at the very early stage of disease before the onset of proteinuria. The findings suggest that ephrin B1 play a critical role for maintaining the barrier function of the glomerular capillary wall. We published these findings as manuscripts in the top journals of Nephrology field, Kidney International and Journal of American Society of Nephrology. We reported some important results at the annual meeting of the American Society of Nephrology held in San Francisco (2007). We are convinced that the results obtained in this project contributed to the better understanding the pathogenic mechanism of proteinuria and to establishment for the novel therapy.
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Research Products
(64 results)
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[Journal Article] The mTOR inhibitor everolimus attenuates the time course of chronic anti-Thyl nephritis in the rat2008
Author(s)
Wittmann, S, Daniel, C, Braun, A, Vogelbacher, R, Shimizu, F, Kawachi, H, Hugo, C
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Journal Title
Nephron Exp Nephrol 108
Pages: 45-56
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Decreased tyrosine phosphorylation of nephrin in rat and human nephrosis2008
Author(s)
Uchida, K, Suzuki, K, Iwamoto, M, Kawachi, H, Ohno, M, Horita, S, Nitta, K
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Journal Title
Kidney Int 73
Pages: 926-932
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Rosuvastatin is additive to high-dose candesartan in slowing progression of experimental mesangioproliferative glomerulosclerosis2008
Author(s)
Kramer, S, Kron, S, Wang-Rosenke, Y, Loof, T, Khadzhynov, D, Morgera, S, Kawachi, H, Shimizu, F, Martini, S, Neumayer, HH, Peters, H
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Journal Title
Am J Physiol Renal Physiol 294
Pages: 801-811
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Eplerenone potentiates the antiproteinuric effects of enalapril in experimental nephrotic syndrome2008
Author(s)
Nakhoul, F, nankin, E, Yaccob, A, Kawachi, H, Karram, T, Awaad, H, Nakhoul, N, Hoffman, A, Abassi, Z
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Journal Title
Am J Physiol Renal Physiol 294
Pages: 628-637
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Nephrin mediates actin reorganization via phosphoinositide 3-kinase in podocytes2008
Author(s)
Zhu, J, Sun, N, Aoudjit, L, Li, H, Kawachi, H, Lemay, S, Takano, T
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Journal Title
Kidney Int 73
Pages: 556-66
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Neutralization of CXCL10 accelerates liver regeneration, in carbon tetrachloride-induced acute liver injury2007
Author(s)
Yoneyama, H, Kai, Y, Koyama, J, Suzuki, K, Kawachi, H, Narumi, S, Ichida, T
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Journal Title
Med Mol Morphol 40
Pages: 191-197
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Podocyte protection by darbepoetin: preservation of the cytoskeleton and nephrin expression2007
Author(s)
Eto ,N, Wada, T, Inagi, R, Takano, H, Shimizu A, Kato, H, Kurihara, H, Kawachi, H, Shankland, SJ, Fujita, T, Nangaku, M
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Journal Title
Kidney Int 72
Pages: 455-63
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Ephrin-B1 localizes at the slit diaphragm of the glomeiular podocyte2007
Author(s)
Hashimoto, T, Karasawa, T, Saito, A, Miyauchi, N, Han, GD, Hayasaka, K, Shimizu, F, Kawachi, H
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Journal Title
Kidney Int 72
Pages: 954-64
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] SM22alpha: the novel phenotype marker of injured glomerular epithelial cells in anti-glomerular basement membrane nephritis2007
Author(s)
Ogawa, A, Sakatsume, M, Wang, X, Sakamaki, Y, Tsubata, Y, Alchi, B, Kuroda, T, Kawachi, H, Narita, I, Shimizu, F, Gejyo, F
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Journal Title
Nephron Exp Nephrol 106
Pages: 77-87
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Blockade of interferon-gamma-inducible protein-10 attenuates chronic experimental colitis by blocking cellular trafficking and protecting intestinal epithelial cells.2007
Author(s)
Suzuki, K, Kawauchi, Y, Palaniyandi, SS, Veeraveedu, PT, Fujii, M, Yamagiwa, S, Yoneyama, H, Han, GD, Kawachi, H, Okada, Y, Ajioka, Y, Watanabe, K, Hosono, M, Asakura, H, Aoyagi, Y, Narumi, S
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Journal Title
Pathol Int 57
Pages: 413-20
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Tumor suppressor carcinoembryonic antigen-related cell adhesion molecule 1 pote-ntates the anchorage-independent growth o human hepatoma HepG2 cells2007
Author(s)
Hokari, M, Matsuda, Y, Wakai, T, Shirai, Y, Sato, M, Tsuchiya, A, Takamura, M, Yamagiwa, S, Suzuki, K, Ohkoshi, S, Ichida, T, Kawachi, H Aoyagi, Y
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Journal Title
Life Sci 81
Pages: 336-345
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Blockade of IP-10/ CXCR3 promotes progressive renal fibrosis2007
Author(s)
Nakaya, I, Wada, T, Furuichi, K, Sakai, N, Kitagawa, K, Yokoyama, H, Ishida, Y, Kondo, T, Sugaya, T, Kawachi, H, Shimizu, F, Narumi, S, Haino, M, Gerard, C, Matsushima, K, Kaneko, S
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Journal Title
Nephron Exp Nephrol 107
Pages: 12-21
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Angiotensin II Type 1 and Type 2 Receptors Play Opposite Roles in Regulating the Barrier Function of Kidney Glomerular Capillary Wall2007
Author(s)
Suzuki, K, Han, GD, Miyauchi, N, Hashimoto, T, Nakatsue, T, Fujioka, Y Koike, H, Shimizu, F, Kawachi, H
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Journal Title
Am J Pathol 170
Pages: 1841-1853
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Expression of allograft inflammatory factor-1 in kidneys: A novel molecular component of podocyte2007
Author(s)
Tsubata, Y, Sakatsume, M, Ogawa, A, Alchi, B, Kaneko, Y, Kuroda, T, Kawachi, H, Narita, I, Yamamoto, T, Gejyo, F
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Journal Title
Kidney Intemational 70
Pages: 1948-54
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Synaptic Vesicle Protein 2B Is Expressed in-Podocyte, and Its Expression Is Altered in Proteinuric Glomeruli2006
Author(s)
Miyauchi, N, Saito, A, Karasawa, T, Harita, Y, Suzuki, K, Koike, H, Han, GD, Shimizu, F, Kawachi, H
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Journal Title
I Am Soci Nephrol 17
Pages: 2748-59
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Nikolic-Paterson DJ, Uchiyama M., Histological differences in newly onset IgA nephropathy between children and adults.2006
Author(s)
Ikezumi, Y, Suzuki, T, Imai, N, Ueno, M, Narita, I, Kawachi, H, Shimizu, F
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Journal Title
Nephrol Dial Transplant 21
Pages: 3466-74
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Rat nephrin modulates cell morphology via the adaptor protein Nck2006
Author(s)
Li, H, Zhu, J, Aoudjit, L, Latreille, M, Kawachi, H, Larose, L, Takano, T
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Journal Title
Biochem Biophys Res Commun 349
Pages: 310-6
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Role of podocyte slit diaphragm as a filtration barrier2006
Author(s)
Kawachi, H, Miyauchi, N, Suzuki, K, Han, GD, Orikasa, M, Shimizu, F
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Journal Title
Nephrology 11
Pages: 274-81
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Contribution of nonproteolytically activated prorenin in glomeruli to hypertensive renal damage2006
Author(s)
Ichihara, A, Kaneshiro, Y, Takemitsu, T, Sakoda, M, Nakagawa, T, Nishiyama, A, Kawachi, H, Shimizu, F, Inagami, T
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Journal Title
J Am Soci Nephrol 17
Pages: 2495-503
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Role of IP-10/CXCL10 in the progression of pancreatitis-like injury in mice after murine retroviral infection2006
Author(s)
Kawauchi, Y, Suzuki, K, Watanabe, S, Yamagiwa, S, Yoneyama, S, Han, GD, Palaniyandi, SS, Veeraveedu, PT, Watanabe, K, Kawachi, H, Okada, Y, Shimizu, F, Asakura, H, Aoyagi, Y, Narumi, S
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Journal Title
Am J Physiol 291
Pages: G345-54
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Transcription factor Ets-1 is essential for mesangial matrix remodeling2006
Author(s)
Mizui, M, Isaka, Y, Takabatake, Y, Sato, Y, Kawachi, H, Shimizu, F, Takahara, S, Ito, T, Imai, E
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Journal Title
Kidney International 70
Pages: 298-305
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Blockade of VEGF accelerates proteinuria, via decrease in nephrin expression in rat crescentic glomerulonephritis2006
Author(s)
Hara, A, Wada, T, Furuichi, K, Sakai, N, Kawachi, H, Shimizu, F, Shibuya, M, Matsushima, K, Yokoyama, H
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Journal Title
Kidney International 69
Pages: 1986-95
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Microarray analysis of a reversible model and an irreversible model of and-Thy-1 nephritis2006
Author(s)
Tsuji, M, Monkawa, T, Yoshino, J, Asai, M, Fukuda, S, Kawachi, H, Shimizu, F, Hayashi, M, Saruta, T
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Journal Title
Kidney International 69
Pages: 996-1004
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Antagonism of PDGF-D by human antibody CR002 prevents renal scarring in experimental glomerulonephritis2006
Author(s)
Ostendorf, T, Rong, S, Boor, P, Wiedemann, S, Kunter, U, Haubold, U, van Roeyen, CR, Eitner, F, Kawachi, H, Starling, G, Alvarez, E, Smithson, G Floege, J
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Journal Title
J Am Soci Nephrol 17
Pages: 1054-62
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Attenuation of mouse acute colitis by naked hepatocyte growth factor gene transfer into the liver2006
Author(s)
Hanawa, T, Suzuki, K, Kawauchi, Y, Takamura, M, Yoneyama, H, Han, GD, Kawachi, H, Shimizu, F, Asakura, H, Miyazaki, J, Maruyama, H Aoyagi, Y
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Journal Title
The Journal of Gene Medicine 8
Pages: 623-35
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Exogenous 5'-nucleotidase improves gloinerular autoregulation in Thy-1 nephritic rats2006
Author(s)
Takenaka, T, Okada, H, Kanno, Y, Inoue, T, Ryuzaki, M, Nakamoto, H, Kawachi, H, Shimizu, F, Suzuki, H
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Journal Title
Am J Physiol 290
Pages: 844-53
Description
「研究成果報告書概要(欧文)」より
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