2007 Fiscal Year Final Research Report Summary
Analysis of IgAl binding protein(BP)in IgA Nephropathy(IgA) patients using Mass Spectrometry(MS)
| Project/Area Number |
18590905
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Kidney internal medicine
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| Research Institution | Juntendo University |
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Principal Investigator |
SUZUKI Yusuke Juntendo University, School of Medicine, Assistant professor (70372935)
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| Project Period (FY) |
2006 – 2007
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| Keywords | IgA nephropathy / Mass spectrometry / IgA binding proten / exogenous antigen |
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| Research Abstract |
Abnormalities of IgA including underglycosylatlon and polymeric WA immune complex (IC) formation are discussed in the pathogenesis of IgA nephropathy (IgAN), but the pathogenesis of this disease still remains unclear. Since upper respiratory infection exercerbates IgAN and tonsillectomy has therapeutic effects on this disease, the previous studies have examined the pathological role of antigens in this disease. However, definitive antigens that form nephritogenic IgAIC have not been identified. To examine whether specific exogenous antigens are involved in IgAIC formation, we analyzed IgA binding protein (BP) from IgAN patients serum by Mass Spectrometry (MS). After isolation of IgA1/IgA1-BP by jacalin, we performed one-dimensional (1D) electrophoresis and analyzed all bands stained with silver. When we compared 6 IgAN patients with 5 healthy volunteers, both groups identified apolipoprotein, immunoglobulin (Ig) heavy chain(α. Μ, γ) and light chain, Ig J chain, fibronectin (FN), Clinhibitor (C1INH), C4 binding protein(C4bp) and α1microglobulin (MG)as IgAl/IgAI-BP by MS. C4bp was frequently observed in the patients group. Moreover, samples before and after the therapies were analyzed by two dimensional difference gel electrophoresis (2D DIGE) system, but we could not identify new protein more than 1D electrophoresis. We further performed Western blot analysis to compare before and after the therapy in patients and healthy volunteers. Contents of IgAl binding C4bp, IgG, and C3 in IgAN patients were higher than those in the volunteers and decreased after the therapy. Although we could not detect exogenous antigen in IgAl-BP, the present findings suggested that IgG, C4bp and C3 in nephritogenic IC might play a key role in the pathogenesis of this disease.
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Research Products
(38 results)
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[Journal Article] Alteration of IgAl glycosylation by down-regulation of core 1 betal, 3-galactosyltransferase and its molecular chaperone Cosmc by Th2 cvtokine2006
Author(s)
Yamada, K., Kobayashi, N., Ikeda, T., Tsuge, T., Suzuki, Y. 他
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Journal Title
Nephrolozy Supple (3), A69
Description
「研究成果報告書概要(欧文)」より
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[Presentation] Mass spectometryを用いたIgA腎症患者血清中のIgA結合蛋白の解析-IgA腎症特異的抗原解明にむけて-2006
Author(s)
尾林, 慶子, 峯木, 礼子, 藤村, 務, 高, ひかり, 鈴木, 祐介, 他
Organizer
第49回日本腎臓学会学術総会
Place of Presentation
東京
Year and Date
2006-06-15
Description
「研究成果報告書概要(欧文)」より
