• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to project page

2007 Fiscal Year Final Research Report Summary

Analyses for mechanisms of progression of kidney diseases by proteinuria

Research Project

Project/Area Number 18590914
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Kidney internal medicine
Research InstitutionKobe Women's University

Principal Investigator

TAKENAKA Masaru  Kobe Women's University, Department of Nutrition and Food science, Professor (20222101)

Co-Investigator(Kenkyū-buntansha) YAMADA Kazuya  Matumoto University, Department of Human Health, Professor (20263238)
KURIHARA Nobutaka  Kobe Women's University, Department of Nutrition and Food science, Professor (10234569)
Project Period (FY) 2006 – 2007
Keywordsproteinuria / oxidative stress / Glia maturation factor
Research Abstract

We have reported that proteinuria induced the expression of brain specific protein, glia maturation factor-β (GMF) in the kidney (latiney Int 2002). It caused vulnerability to oxidative injury, resulting in apoptosis in cultured renal proximal tubular cells (T. Biol. Chem., 2003). To analyze the pathophysiological roles of GMF expression, we constructed transgenic mice expressing GMF (GMF-TG) and induced ischemia-reperfusion (IR) injury.
The real-time PCR revealed that the expression of GMF in the kidney of GMF-TG was increased approximately eight times higher than that in wild type mice (C57BL/6) kidney. We performed Tunel assay to evaluate apoptotic cells in the kidney. It demonstrated that Tunel positive cells were significantly increased in the kidney of GMF-TG, indicating that GMF expression induced apoptosis in vivo, too. Thus, we hypothesized that I/R injury might cause severer damages in GMF-TG mice kidney than in wild type mice kidney. After right nephrectomy, ischemic injury w … More as induced by clamping of left renal artery and vein for 16 min. Surprisingly, serum creatinine levels were significantly low in GMF-TG compared to in normal mice (GMF-TG:0.611±0.07 mg/d1 versus normal:0.841±0.19 mg/d1, p<0.05, n=5 and 6, respectively) at 24hrs after reperfusion. Real-time PCR demonstrated that the expression of several mRNA such as p21, PAI-1 and CTGF in the normal and GMF-TG kidney before induction of I/R injury showed no significant differences. P21, PAI-1 and CTGF expression was up-regulated 24hr after I/R injury in wild type mice kidney (p21:60.3±12.4, PAI-1:90.6±18.2, CTGF:4.9±1.6 fold increases; n=4). It, however, was significantly decreased in GMF-TG kidney (p21:23.3±4.1, PAI-1:39.2±8.4, CTGF:2.4±0.3 fold increases; n=4) compared to those in normal mice at 24hr after 1/R. Data are provided as fold increase of each gene expression in wild type and GMF-TG mice kidney without I/R injury.
Conclusion: The data indicated that GMF expression in vivo might have protective roles on I/R injury, although its expression resulted in apoptosis because of vulnerability to oxidative injury in vitro. Less

  • Research Products

    (18 results)

All 2008 2007

All Journal Article (1 results) (of which Peer Reviewed: 1 results) Presentation (16 results) Book (1 results)

  • [Journal Article] Renoprotective effects of the expression of brain specific protein,GMF-β,in vivo in ischemia-reperfusion injury2007

    • Author(s)
      Kanae Asai, et. al.
    • Journal Title

      Journal of American Society of Nephrology 18巻 Abstract issue

      Pages: 674

    • Description
      「研究成果報告書概要(和文)」より
    • Peer Reviewed
  • [Presentation] 神経特異的因子Glia maturation factor-βはSirt1発現を抑制する2008

    • Author(s)
      中田 杏奈
    • Organizer
      病態栄養学会
    • Place of Presentation
      京都
    • Year and Date
      2008-01-13
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] 神経特異的因子Glia maturation factor-β発現と老化の検討2008

    • Author(s)
      浅井 加奈枝
    • Organizer
      病態栄養学会
    • Place of Presentation
      京都
    • Year and Date
      2008-01-13
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] Glia matration factor-b inhibited the expression of Sirtl.2008

    • Author(s)
      Anna Nakata, et. al.
    • Organizer
      Japanese Society of Metabolism and Clinical Nutrition
    • Place of Presentation
      Kyoto
    • Year and Date
      2008-01-13
    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] Analyses of aging induced by the expression of glia maturation factor-b2008

    • Author(s)
      Kanae Asai, et. al.
    • Organizer
      Japanese Society of Metabolism and Clinical Nutrition
    • Place of Presentation
      Kyoto
    • Year and Date
      2008-01-13
    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] 脂質結合アルブミンによる近位尿細管細胞mProx24の細胞死2007

    • Author(s)
      小川 亜紀
    • Organizer
      分子生物学会・生化学会
    • Place of Presentation
      横浜
    • Year and Date
      2007-12-13
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] 急性腎不全モデルにおけるGlia maturation factor-β発現の影響2007

    • Author(s)
      浅井 加奈枝
    • Organizer
      分子生物学会・生化学会
    • Place of Presentation
      横浜
    • Year and Date
      2007-12-13
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] Expression of brain specific protein, glia maturation factor-b induced apoptosis in cultured renal proximal tubular cells and the kidney of transgenic mice.2007

    • Author(s)
      Yoko Yamashita, et. al.
    • Organizer
      The molecular biology society of Japan
    • Place of Presentation
      Yokohama
    • Year and Date
      2007-12-13
    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] Renoprotective effects of the expression of brain specific protein, GMF-b, in vivo in ischemia-reperfusion injury.2007

    • Author(s)
      Kanae Asai, et. al.
    • Organizer
      American Society of Nephrology
    • Place of Presentation
      San Francisco USA
    • Year and Date
      2007-11-04
    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] 高度変化による血圧変動と高血圧素因2007

    • Author(s)
      稲留 哲也
    • Organizer
      高血圧学会
    • Place of Presentation
      沖縄
    • Year and Date
      2007-10-26
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] Expression of brain specific protein, glia maturation factor-b induced apoptosis in cultured renal proximal tubular cells and the kidney of transgenic mice.2007

    • Author(s)
      Yoko Yamashita, et. al.
    • Organizer
      Japanese Society of Nephrology
    • Place of Presentation
      Hamamatu
    • Year and Date
      2007-05-25
    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] 脂質結合アルブミンによる細胞死はビタミンC,Eにより阻害される2007

    • Author(s)
      小川 亜紀
    • Organizer
      栄養食糧学会
    • Place of Presentation
      京都
    • Year and Date
      2007-05-20
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] 急性腎不全モデルにおけるコエンザイムQ10の効果2007

    • Author(s)
      浅井 加奈枝
    • Organizer
      栄養食糧学会
    • Place of Presentation
      京都
    • Year and Date
      2007-05-20
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] Vitamin C and E inhibited apoptosis induced by Oleic acid bounded-albumin.2007

    • Author(s)
      Aki Ogawa, et. al.
    • Organizer
      Japanese Society of Nutrition and Food Science
    • Place of Presentation
      Kyoto
    • Year and Date
      2007-05-20
    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] The effects of CoQ10 in acute renal failure2007

    • Author(s)
      Kanae Asai, et. al.
    • Organizer
      Japanese Society of Nutrition and Food Science
    • Place of Presentation
      Kyoto
    • Year and Date
      2007-05-20
    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] Analyses of anti-oxidative stress by CoQ102007

    • Author(s)
      Kanae Asai, et. al.
    • Organizer
      Japanese Society of Metabolism and Clinical Nutrition
    • Place of Presentation
      Kyoto
    • Year and Date
      2007-01-13
    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] Oleic acid bounded-albumin induced apoptosis in cultured proximal tubular cells, mProx24.2007

    • Author(s)
      Aid Ogawa, et. al.
    • Organizer
      Japanese Society of Metabolism and Clinical Nutrition
    • Place of Presentation
      Kyoto
    • Year and Date
      2007-01-13
    • Description
      「研究成果報告書概要(欧文)」より
  • [Book] Principles of Medicine for Nutritionist2007

    • Author(s)
      Masaru Takenaka
    • Publisher
      Ishiyaku Publishers, Inc.
    • Description
      「研究成果報告書概要(欧文)」より

URL: 

Published: 2010-02-04  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi