Identification of epitopes of autoantibodies in multiple sclerosis

2007 Fiscal Year Final Research Report Summary

• Project/Area Number: 18590949
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Neurology
• Research Institution: Saga University
• Principal Investigator: KURODA Yasuo Saga University, Faculty of Medicine, Professor (30117105)
• Co-Investigator(Kenkyū-buntansha): SUEOKA Eizaburou Saga University, Faculty of Medicine, Associate Professor (00270603) ; SUEOKA Naoko Saga University, Faculty of Medicine, Assistant Professor (20321846) ; YUKITAKE Motohiro Saga University, Faculty of Medicine, Assistant Professor (10304891)
• Project Period (FY): 2006 – 2007
• Keywords: multiple sclerosis / hnRNP A2 / B1 / cerebrospinal fluid / HAM / epitope / autoantibody / demyelinating encephalitis / disease marker

Research Abstract

We performed a blind study on the association between anti-hnRNP A2/B1 antibody and multiple sclerosis. We assayed anti-hnRNP A2/B1 protein antibody in 105 cerebrospinal fluid samples from patients affected neurological disorders. Both anti-hnRNP A Al proteinantibdy and anti-hnRNP A2/B1 protein antibody were significantly frequently found in cerebrospinal fluid of multiple sclerosis patients than those with other neurological diseases. We next made a study on the epitope of hnRNP B1 protein. We made fragments of hnRNP B1 protein, and then analyzed reactivity of cerebrospinal fluid of multiple sclerosis against those peptides by use of Western immunoblots. We identified anti-hnRNP B1 protein antibody reacted with the amino acid sequence between 234 and 305 of hnRNP B1 protein.

Research Products

• [Journal Article] 髄液内抗hnRNP B1抗体と多発性硬化症 (2006)
  • Author(s): 雪竹 基弘
  • Journal Title: 神経治療学 23 Pages: 99-105
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Significantly increased antibody response ton heterogeneous nuclear ribo nucleoproteins in cerebrospinal fluid of multiple sclerosis patients but not in patients with human T-lymphotropic virus type-I-associated myelopathy/tropical spastic paraparesis (2006)
  • Author(s): Yukitake M, Sueoka E, Sueoka N, Sato A, Ohashi H, Yakushiji Y, Saito M, Osame M, Izumo S, Kuroda Y
  • Journal Title: Journal of Neurovirology 14 Pages: 130-135
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Cerebrospinal fluid anti-hnRNP B1 antibody and multiple Sclerosis (2006)
  • Author(s): Yukitake, M.
  • Journal Title: Shinkeichiryougaku 23 Pages: 99-105
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Significantly increased antibody Response to heterogeneous nuclear ribonucleoproteins in cerebrospinal fluid of multiple sclerosis patients but not in patients with human T-lmyphotropic virus type-I-associated myelopathy/tropica ; spastic Paraparesis (2006)
  • Author(s): Yukitake, M., Sueoka, E., Sueoka, N., Sato, A., Ohashi, H., Yakushiji, Y., Saito, M., Osame, M., Izumo, S., Kuroda, Y
  • Journal Title: Journal of Neurovirology 14 Pages: 130-135
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Daignostic value of CSF autoantibodies against heterogeneous nuclear ribonucleoprotein A1 and A2B1 in multiple sclerosis patients (2006)
  • Author(s): Yukitake M, Sueoka E, Sueoka N, Kuroda Y
  • Organizer: 8th International Congress of Neuroimmunolgy
  • Place of Presentation: Nagoya,Japan
  • Year and Date: 2006-10-16
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Diagnostic value of CSF autoantibodies against heterogeneous nuclear ribonucleoprotein Al and in multiple sclerosis patients (2006)
  • Author(s): Yukitake M., Sueoka E., Sueoka, N., Kuroda, Y
  • Organizer: 8th International Congress of Neuroimmunology
  • Place of Presentation: Nagoya, Japan
  • Year and Date: 2006-10-06
  • Description: 「研究成果報告書概要(欧文)」より