2007 Fiscal Year Final Research Report Summary
Role of intercellular electrical coupling in neurological disorders : elucidation of gap junction funciotn
| Project/Area Number |
18590962
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Neurology
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| Research Institution | University of Occupational and Environmental Health, Japan |
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Principal Investigator |
TSUJI Sadatosi University of Occupational and Environmental Health, Japan, School of Medicine, Professor (30117171)
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| Co-Investigator(Kenkyū-buntansha) |
TAKENORI Uozumi University of Occupational&Environmental Health, Japan, School of Medicine, Associate Professor (00160226)
AKAMATSU Naoki University of Occupational&Environmental Health, Japan, School of Medicine, Assistant Professor (10299612)
YUHI Tomoaki University of Occupational&Environmental Health, Japan, School of Medicine, Research Associate (60330982)
OKADA Kazumasa University of Occupational&Environmental Health, Japan, School of Medicine, Research Associate (30341499)
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| Project Period (FY) |
2006 – 2007
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| Keywords | gap junction / connexin / very fast oscillation / EL mouse / epilepsy / ELマウス |
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| Research Abstract |
We investigated the effect of the gap junction blocks; carbenoxolone (100mM) on epileptiform bursting induced by bicuculline (GABAA receptor blocker), 0-Me^<2+> and high KE (9.25m) in genetically epileptic EL mouse hippocampal slices. Carbenoxolone abolished bicuculline and high-K^+-induced epileptiform activities completely and decreased the frequency of 0- Mg^<2+>induced epileptiform bursts.
These results were almost same as previously reported in normal mice and rats. We conclude that gap junction contrlute to the prolongation of epileptiform discharges in EL mouse just as in normal mouse.
In patients with subdural electrodes implanted for the treatment of medically intractable epilepsy, we investigated the inter-cortical connection of the epileptogenic regions. The cortical electroencephalographic recording revealed that the epileptogenic network consists of independent epileptogenic areas. The strength of the interepileptogenic area connectivities were different on each connection. The gap junction may play a role in the interareal neuronal connectivity.
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