2007 Fiscal Year Final Research Report Summary
Analysis of proteins which specifically binds to thyroid hormone receptor β
| Project/Area Number |
18591018
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Endocrinology
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| Research Institution | Nagoya University |
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Principal Investigator |
KANOU Yasuhiko Nagoya University, Research Instiiut of Environmental Medicine, Assistant Professor (50252292)
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| Co-Investigator(Kenkyū-buntansha) |
HAYASHI Yoshitaka Nagoya University, Research Inslilute of Fnvimnmental Medicine, Associate professor (80420363)
TAKAGISHI Yoshiko Nagoya University, Research Institute of Envimnmenlal Medicine, Assistant professor (50024659)
MURATA Yoshiharu Nagoya University, Research Institute of Environmental Medicine, Professor (80174308)
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| Project Period (FY) |
2006 – 2007
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| Keywords | Resistance to thyroid hormone / thyroid hormone receptors / co-activator / Yeast two-hybrid system / cDNA / cloning |
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| Research Abstract |
There is a case where any mutations are not found in two types of thyroid hormone receptor(TR) in the resistance to thyroid hormone. We hypothesized that a specific coactivator for thyroid hormone receptor b existed. This coactivator may have any abnormality in the resistance to thyroid hormone. We tried to identify the cDNA for the specific protein. Yeast two-hybrid system was applied to screen a human liver cDNA library. We found two cDNA clones, No.60 and 93, as a candidate of TR-specific coactivator.
1. A clone 60 was a fragment derived from 3 kinds of mRNA. The full-length cDNAs for these mRNAs were cloned. The deduced amino acid sequences showed that proteins had nuclear transport signals.
2. In amino acid sequences of clone 60 proteins, there was a high homology sequence for another nuclear protein.
3. To determine whether a clone 60 protein binds to TRb, pull-down assay was occurred. TRb and a clone 60 protein were expressed as a GST-fusion protein and His-tag fusion protein, respectively. Both fusion protein were mixed and precipitated with specific antibodies. Western blotting showed both fusion proteins bound in vitro.
4. A clone 93 could not be expressed as His-tag fusion protein in vitro. We are advancing the analysis of the clone 60 proten further continuously.
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[Journal Article] Insertion on an intracistemal A particle retrotransposon element in plasma membrane calcium ATPase 2 gene attenuates its expression and produces an ataxic phenotype in joggle mutant mice2008
Author(s)
Sun, XY., Hayashi, Y., Kanou, Y., Takagishi, Y., Murata, Y., et. al.
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Journal Title
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Insertion of an intracisternal A particle retrotransposon element in plasma membrane calcium ATPase 2 gene attenuates its ecpression and produces an2007
Author(s)
Sun, XY., Hayashi, Y., Kanou, Y., Takagishi, Y., Murata, Y.,
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Journal Title
Description
「研究成果報告書概要(和文)」より
Peer Reviewed
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[Journal Article] Mapping of jog locus to the region between D6Mit104 and D6Mit336 on mouse chromosome 62007
Author(s)
Sun, XY., Kanou, Y., Takagishi, Y., Hayashi, Y., Murata, Y., et. al.
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Journal Title
Experimental Animal 56
Pages: 389-392
Description
「研究成果報告書概要(欧文)」より
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[Presentation] The expression and role of thyroid hormone responsive gene, ZAKI-4, during brain development2008
Author(s)
Kanou, Y., Hayasaka, S., Sun, XY., Tang, YP., Takagishi, Y., Hayashi, Y., Murata, Y
Organizer
The Japan Endocrine Society
Place of Presentation
Tokyo
Year and Date
2008-06-14
Description
「研究成果報告書概要(欧文)」より
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