2007 Fiscal Year Final Research Report Summary
Development of human monoclonal antibody molecule for patients with highly pathogenic avian influenza(H5N1)
| Project/Area Number |
18591120
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
膠原病・アレルギー・感染症内科学
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| Research Institution | Nagasaki University |
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Principal Investigator |
YAMASHIRO Tetsu Nagasaki University, Center for International Collaborative Research, Professor (00244335)
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| Co-Investigator(Kenkyū-buntansha) |
MORI Kouichi Nagasaki University, Institute of Tropical Medicine, Professor (40182240)
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| Project Period (FY) |
2006 – 2007
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| Keywords | phage display / highly pathogenic avian influenza / H5N1 / Emerging and Re-emerging infectious diseases |
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| Research Abstract |
Passive antibody therapy against H5N1 viruses could be a potentially adjunctive treatment option in human cases with H5N1 infection, because patients with Spanish flu pneumonia who had received transfusion with convalescent human serum products have been reported to reduce the risk of death. The objective of the study is to identify human monoclonal Fab with neutralizing potency against influenza A subtype H5N1 stains. Previously, we have constructed a human combinatorial Fab library using peripheral lymphocytes(PBLs) of a volunteer who had once infected with influenza A H5N1 and recovered. Identification of human Fab clones with neutralizing potency against HPAI H5N1 strains has been attempted using phage display technique with inactivated H5N1 virions isolated in Vietnam for biological enrichment(panning) and screening, however, was not successful. Recently, we have obtained a larger amount of PBLs from a healthy volunteer who had contracted H5N1 infection in May 2007 and recovered. Serum of the volunteer from which PBLs were isolated showed a high HI titer against an H5N1 year 2007 strain tested. A new human Fab library has been being constructed and efficient isolation of human monoclonal Fabs with neutralizing potency against H5N1 strains would be expected.
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Research Products
(26 results)
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[Journal Article] Identification of a human monoclonal Fab with neutralizing activity against H3N2 influenza A Strain from a newly constructed human Fab library2008
Author(s)
Shibuya, T., Yamasbiro, T., Masaike, Y., Ohuchi, M., Uechi, G, Nishizono, A.
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Journal Title
Microbiology and Immunology 52
Pages: 162-170
Description
「研究成果報告書概要(和文)」より
Peer Reviewed
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[Journal Article] Identification of a human monoclonal Fab with neutralizing activity against H3N2 influenza A strain from a newly constructed human Fab library2008
Author(s)
Shibuya, T., Yamashiro, T., Masaike, Y., Ohuchi, M., Uechi, G., Nishizono, A
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Journal Title
Microbiology and Immunology 52
Pages: 162-170
Description
「研究成果報告書概要(欧文)」より
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[Presentation] The collaborative study on emerging and re-emerging infectious diseases in Vietnam : Enhancement of Research Capacity2007
Author(s)
Tetsu, Yamashiro, Kouichi, Morita, Shigeru, Katamine, Masahiro, Takagi, Osamu, Nakagomi, Koya, Ariyoshi, Kouichiro, Kudoh, Naoto, Keicho, Shinichi, Oka, Kenji, Hirayama
Organizer
The 2nd Thailand Japan Joint-Forum on infectious diseases
Place of Presentation
Thailand
Year and Date
20071008-09
Description
「研究成果報告書概要(欧文)」より
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[Presentation] Construction of Human Fab and ScFv Libraries and Molecular Cloning of Human Anti-Influenza Virus A Subtype H5N1 Monoclonal Molecules2007
Author(s)
Gen-ichiro, Uechi, Le, Thi Quynh, Mai, Doan, Hai Yen, Nguyen Le, Khanh Hang, Kouichi, Morita, Tetsu, Yamashiro
Organizer
Asian Research Forum 2007
Place of Presentation
Nagasaki
Year and Date
2007-01-15
Description
「研究成果報告書概要(欧文)」より
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[Presentation] The collaborative study on emerging and re-emerging infectious diseases in Vietnam Enhancement of Research Capacity2006
Author(s)
Tetsu Yamashiro, Kenji Hirayama, Kouichi Morita, Shigeru Katamine, Masahiro Takagi, Osamu Nakagomi, Koya Ariyoshi, Kouichiro Kudoh, Naoto Keicho, Satoshi Kimura, Yoshiki Aoki.
Organizer
Joint International topical Medicine Meetink(JITMM)
Place of Presentation
Thailand
Year and Date
2006-11-29
Description
「研究成果報告書概要(和文)」より
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[Presentation] The collaborative study on emerging and re-emerging infectious diseases in Vietnam : Enhancement of Research Capacity2006
Author(s)
Tetsu, Yamashiro, Kenji, Hirayama, Kouichi, Morita, Shigeru, Katamine, Masahiro, Takagi, Osamu, Nakagomi, Koya, Ariyoshi, Kouichiro, Kudoh, Naoto, Keicho, Satoshi, Kimura, Yoshiki, Aoki
Organizer
Joint International Tropical Medicine Meeting(JITMM)
Place of Presentation
Thailand
Year and Date
2006-11-29
Description
「研究成果報告書概要(欧文)」より
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