2009 Fiscal Year Final Research Report
Role of natural killer cells on varicella zoster virus infection
| Project/Area Number |
18591205
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Pediatrics
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| Research Institution | Kawasaki Medical School |
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Principal Investigator |
TERADA Kihei Kawasaki Medical School, 医学部, 教授 (50172094)
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| Co-Investigator(Kenkyū-buntansha) |
OGITA Satoko 川崎医科大学, 医学部, 講師 (40309555)
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| Project Period (FY) |
2006 – 2009
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| Keywords | 水痘帯状疱疹ウイルス / NK細胞 |
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| Research Abstract |
Cell tropism of varicella-zoster virus (VZV) is very strong, so that VZV is difficult to be recovered without cells. And there have been some reports about lymphocyte-associated-viremia during VZV infection. In order to detect strength of cell tropism between virus and lymphocyte subsets and/or which lymphocytes are mainly infected, we investigated them in vitro on peripheral blood mononuclear cells. After incubation with VZV Oka strain, we sorted peripheral blood lymphocytes into T, B and NK cells with FACS. And then we measured quantity of VZV copy numbers with real-time PCR. As a result, there were the highest DNA copies in the NK subsets, B cell subsets in second. Next, between NK cells and non-NK cells, the DNA copies were significantly higher in NK cells than those in non-NK cells. Among CD16, CD56 and CD57 subsets of NK cells, there were CD16>CD56, CD16>CD57, CD56>CD57 in infected DNA copy numbers. This suggests that CD16 of NK cells are the strongest cell tropism to VZV. And change of cell size is thought to be associated with VZV infection and apoptosis of the infected cells.
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[Journal Article] Localized Intraabdominal Kikuchi-Fujimoto Disease: a case report and review of the Literature.2008
Author(s)
Teranisi Hideto, Kawasaki Kozo, Sasaki Atsuko, Fujimoto Hiroki, Inoue Mika, Monju Ayaka, Wakabayashi Tokio, Akaike Hideto, Yamaguchi Tetsuya, Ogita Satoko, Oda Keiko, Terada Kihei, Kazunobu Ouchi.
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Journal Title
Kawasaki Med J 34(4)
Pages: 285-289
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[Journal Article] Molecular and clinical analyses of Japanese patients with carbamylphoshate synthetase 1 (CPS1) deficiency.2007
Author(s)
Kurokawa Keiji, Yorifuji Tohru, Kawai Masahiko, Momoi Toru, Nagasaka Hironori, Takayanagi Masaki, Kobayashi Keiko, Yoshino Makoto, Kosho Tomoki, Adachi Masanori, Otsuka Harumi, Yamamoto Shigenori, Murata Toshiaki, Suenaga Akihito, Ishii Tsutomu, Terada Kihei, et al.
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Journal Title
Journal of Human Genetics 52(4)
Pages: 349-354
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