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2007 Fiscal Year Final Research Report Summary

The effect of intrauterine inflammation on fetal cerebral hemodynamics and white matter injury in the chronically instrumented fetal sheep

Research Project

Project/Area Number 18591213
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Embryonic/Neonatal medicine
Research InstitutionTohoku University

Principal Investigator

MATSUDA Tadashi  Tohoku University, Tohoku University Hospital, Associate Professor (50361100)

Co-Investigator(Kenkyū-buntansha) WATANABE Tatsuya  Tohoku University Hospital, 病院, Associate Professor (70400380)
HANITA Takushi  Tohoku University Hospital, 病院, Senior Residents (30400360)
Project Period (FY) 2006 – 2007
Keywordsintrauterine inflammation / cerebral blood flow / brain sparing effect / cerebral ischemia / cerebral white matter damage / periventricular leukomalacia / fetus / sheep
Research Abstract

We developed an animal model of fetal cerebral white matter injury (WMl) associated with inflammation in stem to clarify the pathogenesis of and preventive measures against it using chronically instrumented fetal sheep; intrauterine inflammation, induced by the infusion with intravenous granulocyte-colony stimulating factor and intra-amniotic endotoxin, led fetal hypoxia and then resulted in a compensatory increase of fetal cerebral blood flow, i. e., brain span effect (BSE). Subsequently, at 24 hours after the endotoxin infusion, anemic hypoxia as a hemodynamic insult induced sudden cessation of BSE and resulted in focal WMI. In the current experiment using this model, fetal sheep were divided into 4 groups. In an oxygen supplement group (n=3), the hemodynamic insult was performed after the BSE bad been already cancelled by maternal oxygen supplement. In an anti-inflammation group (n=2), fetuses were administered dexamethasone simultaneously with the BSE induction before the hemodynamic insult. In a pan-inflammation group (n=2), anemic hypoxia was done again after the initial anemic insult induced the BSE without the inflammatory preparation. Neither inflammatory nor hypoxic insult was done in a control group (n 3). Consequently, periventricular focal and/or subcortical multi-focal WMI was found in 2 of the 3 fetuses in the oxygen supplement group, 1 of the 2 in the anti-inflammation group and 2 of the 2 in the non-inflammation group but none in the control group. Based on these results, it was considered that (1) sudden cessation of BSE, even if induced by inflammation, or hypoxia, might be essential in the genesis of fetal cerebral WMI and (2) both oxygen supplement and dexamethasone might be unable to prevent the development of the WMI associated with BSE.

  • Research Products

    (6 results)

All 2008 2007

All Journal Article (3 results) (of which Peer Reviewed: 1 results) Presentation (2 results) Book (1 results)

  • [Journal Article] Induction of necrotizing funisitis by fetal administration of intravenous granulocyte-colony stimulating factor and intra-amniotic endotoxin in premature fetal sheep.2007

    • Author(s)
      Watanabe T, Matsuda T, Hanita T, Okuyama K, Cho K, Kobayashi K, Kobayashi Y.
    • Journal Title

      Pediatr Res 62

      Pages: 670-673

    • Description
      「研究成果報告書概要(和文)」より
    • Peer Reviewed
  • [Journal Article] induction of necrotizing fuhisitis by fetal administration of intravenous granulocyte-colony stimulating factor and intra-amniotic endotoxin in premature fetal sheep2007

    • Author(s)
      Watanabe T, Matsuda T, Hanita T, Okuyama K, Cho K, Kobayashi K, Kobayashi Y.
    • Journal Title

      Pediatr Res 62

      Pages: 670-673

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Funisitis2007

    • Author(s)
      Tadashi Matsuda
    • Journal Title

      Medical View, Co, . Ltd., Preterm Labor

      Pages: 50-59

    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] ヒツジ胎仔を用いた子宮内炎症がびまん性脳白質傷害に与える影響の解析2008

    • Author(s)
      北西龍太, 松田 直
    • Organizer
      第36回胎児新生児神経研究会
    • Place of Presentation
      山形県上山市月岡ホテル
    • Year and Date
      2008-02-10
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] The effect of intrauterine inflammation on fetal cerebral hemodynamics and white matter injury in the chronically instrumented fetal sheep2008

    • Author(s)
      Kitanishi Ryuta, Tadashi matsuda
    • Organizer
      The 36^<th> Fetal and Neonatal Neurological Society
    • Place of Presentation
      kaminoyama city, Yamagata, JAPAN
    • Year and Date
      2008-02-10
    • Description
      「研究成果報告書概要(欧文)」より
  • [Book] 「早産-最新の知見と取り扱い」, 臍帯炎2007

    • Author(s)
      松田 直(分担執筆), 佐藤和雄(監修)
    • Total Pages
      50-59
    • Publisher
      メジカルビュー社
    • Description
      「研究成果報告書概要(和文)」より

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Published: 2010-02-04  

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