Effects of maternal care on brain development : molecular mechanism of mother-infant interactions

2009 Fiscal Year Final Research Report

• Project/Area Number: 18591220
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Embryonic/Neonatal medicine
• Research Institution: Kagawa University
• Principal Investigator: MIKI Takanori Kagawa University, 医学部, 准教授 (30274294)
• Co-Investigator(Kenkyū-buntansha): YOKOYAMA Toshifumi 神戸大学, 農学部, 助教 (10380156) ; MATSUMOTO Yoshiki 香川大学, 医学部, 助教 (90335844) ; WARITA Katsuhiko 香川大学, 医学部, 助教 (40452669) ; TAKEUCHI Yoshiki 香川大学, 医学部, 教授 (20116619) ; KUSAKA Takashi 香川大学, 医学部附属病院, 講師 (50274288) ; ITOH Susumu 香川大学, 医学部, 教授 (80145052)
• Project Period (FY): 2006 – 2009
• Keywords: 母仔分離 / ストレス / 養育環境 / 母子間相互作用 / 脆弱性分子基盤 / 神経栄養因子 / 脳・神経発達 / 精神神経機能

Research Abstract

Wistar male rats were separated from their mothers for 3 hours each day between PND10 and 15 (MD group). Nurotrophin ligand and receptor expressions were analyzed. The expression of BDNF/TrkB in MD rats was enhanced in both mRNA and protein levels. In contrast, no significant difference in NGF/TrkA expression level was observed between these groups. On the other hand, OMgp expression was elevated in MD animals. Abnormal fluctuations in those trophic factors cause organic changes, and ultimately lead to functional disturbance.

Research Products

• [Journal Article] Alcohol or Their Combination on Cholinergic Markers in the Frontal Cortex and Hippocampus of Rat. (2010)
  • Author(s): Jamal M, Ameno K, Miki T, Tanaka N, Ohkubo E, Kinoshita H. Effects of Systemic Nicotine
  • Journal Title: Neurochem Res. (in press)
  • Peer Reviewed
• [Journal Article] Effects of short-term ethanol ingestion on the expression of neurotrophins and their receptors: No changes in the expression of the glial-derived neurotrophic factor in the mouse hippocampus (2010)
  • Author(s): Ueno T, Miki T, Wang ZY, Yokoyama T, Lee KY, Kusaka T, Warita K, Sumitani K, Matsumoto Y, Yakura T, Liu JQ, Bedi KS, Takeuchi Y.
  • Journal Title: Current Neurobiol. 1 Pages: 40-45
  • Peer Reviewed
• [Journal Article] GABA expression in c-Fos immunoreactive neurons of the rat periaqueductal gray induced by electroacupuncture at the point of Zusanli. (2010)
  • Author(s): Fusumada K, Yokoyama T, Miki T, Matsumoto Y, Warita K, Wang ZY, Yakura T, Liu JQ, Takeuch Y.
  • Journal Title: Current Neurobiol. 1 Pages: 10-13
  • Peer Reviewed
• [Journal Article] Short-term exposure to ethanol causes a differential response between nerve growth factor and brain-derived neurotrophic factor ligand/receptor systems in the mouse cerebellum (2010)
  • Author(s): Wang ZY, Miki T, Lee KY, Yokoyama T, Kusaka T, Sumitani K, Warita K, Matsumoto Y, Yakura T, Hosomi N, Ameno K, Bedi KS, Takeuchi Y
  • Journal Title: Neuroscience 165 Pages: 485-491
  • Peer Reviewed
• [Journal Article] Cholinergic alterations following alcohol exposure in the frontal cortex of Aldh2-deficient mice models (2009)
  • Author(s): Jamal M, Ameno K, Miki T, Wang W, Kumihashi M, Isse T, Kawamoto T, Kitagawa K, Nakayama K, Ijiri I, Kinoshita H.
  • Journal Title: Brain Res. 27 Pages: 37-43
  • Peer Reviewed
• [Journal Article] Relationship between cerebral oxygenation and phosphorylation potential during secondary energy failure in hypoxic-ischemic newborn piglets (2009)
  • Author(s): Kusaka T, Ueno M, Miki T, Kuboi T, Nakamura S, Koyano K, Ijichi S, Yasuda S, Okubo K, Kawada K, Namba M, Nishida T, Imai T, Isobe K, Itoh S.
  • Journal Title: Pediatr Res. 65 Pages: 317-22
  • Peer Reviewed
• [Journal Article] Early postnatal ethanol exposure induces fluctuation in the expression of BDNF mRNA in the developing rat hippocampus. (2008)
  • Author(s): Miki T, Kuma H, Yokoyama T, Sumitani K, Matsumoto Y, Kusaka T, Warita K, Wang ZY, Hosomi N, Imagawa T, Bedi KS, Itoh S, Nakamura Y, Takeuchi Y
  • Journal Title: Acta Neurobiol Exp. 68 Pages: 484-493
  • Peer Reviewed
• [Journal Article] Ethanol neurotoxicity and dentate gyrus development. (2008)
  • Author(s): Miki T, Yokoyama T, Sumitani K, Kusaka T, Warita Y, Matsumoto Y, Wang ZY, Wilce PA, Bedi KS, Itoh, S, Takeuchi Y
  • Journal Title: Congenital Anomalies 48 Pages: 110-117
  • Peer Reviewed
• [Journal Article] Characterization of neuroprogenitor cells expressing the PDGF beta-receptor within the subventricular zone of postnatal mice (2008)
  • Author(s): Ishii Y, Matsumoto Y, Watanabe R, Elmi M, Fujimori T, Nissen J, Cao Y, Nabeshima YI, Sasahara M, Funa K
  • Journal Title: Mol Cell Neurosci. 37 Pages: 507-518
  • Peer Reviewed
• [Journal Article] Activin A and equine chorionic gonadotropin recover reproductive dysfunction induced by neonatal exposure to an estrogenic endocrine disruptor in adult male mice. (2008)
  • Author(s): Warita K, Okamoto K, Mutoh KI, Hasegawa Y, Yue ZP, Yokoyama T, Matsumoto Y, Miki T, Takeuchi Y, Kitagawa H, Sugawara T, Hoshi N.
  • Journal Title: Biol Reprod. 78 Pages: 59-67
  • Peer Reviewed
• [Journal Article] Changes in cholinergic function in the frontal cortex and hippocampus of rat exposed to ethanol and acetaldehyde (2007)
  • Author(s): Jamal M, Ameno K, Ameno S, Morishita J, Wang W, Kumihashi M, Ikuo U, Miki T, Ijiri I
  • Journal Title: Neuroscience 144 Pages: 232-238
  • Peer Reviewed
• [Journal Article] The effect of prenatal X-irradiation on the developing cerebral cortex of rats. A quantitative assessment of glial cells in the somatosensory cortex (2007)
  • Author(s): Miki T, Yokoyama T, Sumitani K, Wang ZY, Yang W, Kusaka T, Matsumoto Y, Warita K, Lee NS, Fukui Y, Takeuchi Y
  • Journal Title: Int J Dev Neurosci. 25 Pages: 293-297
  • Peer Reviewed
• [Journal Article] Oligodendrocyte myelin glycoprotein (OMgp) in rat hippocampus is depleted by chronic ethanol consumption (2006)
  • Author(s): Okamoto H, Miki T, Lee KY, Yokoyama T, Kuma H, Wang ZY, Gu H, Li HP, Matsumoto Y, Irawan S, Bedi KS, Nakamura Y, Takeuchi Y
  • Journal Title: Neurosci Lett. 406 Pages: 76-80
  • Peer Reviewed
• [Journal Article] Age-related changes in growth hormone-immunoreactive cells in the anterior pituitary gland of Jcl: Wistar-TgN (ARGHGEN) 1Nts rats (Mini rats) (2006)
  • Author(s): Matsumoto Y, Tsukamoto Y, Miki T, Ogawa K, Lee KY, Yokoyama T, Satriotomo I, Li HP, Gu H, Wang ZY, Karasawa S, Ueda S, Sasaki F, Takeuchi Y.
  • Journal Title: Congenit Anom. (Kyoto) 46 Pages: 188-93
  • Peer Reviewed
• [Journal Article] 母性剥奪症候群(Maternal Deprivation Syndrome)の発達神経科学的アプローチ
  • Author(s): 三木崇範
  • Journal Title: 日本未熟児新生児学会雑誌 第20巻第1号 Pages: 35-40
  • Peer Reviewed
• [Journal Article] 子供の脳発達と養育環境-母性剥奪症候群のエピジェネティック解析
  • Author(s): 三木崇範
  • Journal Title: 財団法人成長科学協会研究年報 第31巻 Pages: 189-190
  • Peer Reviewed
• [Presentation] 短期間アルコール摂取マウス小脳の神経栄養因子リガンド/レセプター系の特異的応答性 (2010)
  • Author(s): 三木崇範、汪治宇、横山俊史、日下隆、住谷和則、割田克彦、松本由樹、矢倉富子、劉俊騫、王朝暉、竹内義喜
  • Organizer: 第115回日本解剖学会
  • Place of Presentation: 岩手県民会館
  • Year and Date: 20100328-20100330
• [Presentation] Non-vesicular synaptic transport: WGA- HRP and Rab3A-siRNA studies (2010)
  • Author(s): Yakura T, Matsumoto Y, Miki T, Warita K, Wang ZY, Liu JQ, Mamoru F, Takeuchi Y.
  • Organizer: 第115回日本解剖学会
  • Place of Presentation: 岩手県民会館
  • Year and Date: 20100328-20100330
• [Presentation] A protein conjugation of WGA essential for transsynaptic tracer in neuronal system: application for Amyloid-βin vivo (2009)
  • Author(s): Takeuchi Y, Matsumoto Y, Miki T, Warita K, Wang ZY, Yakura T, Liu JQ.
  • Organizer: International Symposium on Early Detection and Rehabilitation Technology of Dementia
  • Place of Presentation: 岡山大学
  • Year and Date: 20091211-20091212
• [Presentation] WGA結合タンパクがもたらすシナプス膜輸送機構の新展開 (2009)
  • Author(s): 松本由樹、三木崇範、割田克彦、汪治宇、矢倉富子、劉俊騫、竹内義喜
  • Organizer: 第64回日本解剖学会地方会
  • Place of Presentation: 高知大学
  • Year and Date: 20091024-20091025
• [Presentation] WGA-HRPによる順行性シナプス輸送:ラット迷走神経へのRab3A-siRNA投与 (2009)
  • Author(s): 竹内義喜、松本由樹、三木崇範、横山俊史、割田克彦、汪治宇、矢倉富子、藤田守
  • Organizer: 第32回日本神経科学大会
  • Place of Presentation: 名古屋国際会議場
  • Year and Date: 20090916-20090918
• [Presentation] Modulation of hypothalamopituitary axis in Mini rat: further studies with hypophysiotropic somatostatin and gonadotroph (2009)
  • Author(s): Matsumoto Y, Miki T, Tsukamoto Y, Warita K, Wang ZY, Yakura T, Karasawa S, Ueda S, Takeuchi Y.
  • Organizer: The 8th World Congress on Neurohypophysial Hormones
  • Place of Presentation: 北九州国際会議場
  • Year and Date: 20090904-20090908
• [Presentation] evaluation of anterograde synaptic transport of WGA-HRP in the rat (2009)
  • Author(s): 竹内義喜、三木崇範、横山俊史、松本由樹、割田克彦、汪治宇、上野貴士、藤田守
  • Organizer: 第114回日本解剖学会
  • Place of Presentation: 岡山理科大学
  • Year and Date: 20090328-20090330
• [Presentation] Early postnatal ethanol exposure alters the BDNF and neural interactions in the olfactory bulb. (2008)
  • Author(s): 松本由樹、三木崇範、谷賀、割田克彦、汪治宇、竹内義喜
  • Organizer: 第113回日本解剖学会
  • Place of Presentation: 大分大学
  • Year and Date: 20080327-20080329
• [Presentation] 母性剥奪症侯群(Maternal Deprivation Syndrome)の発達神経科学的アプローチ (2007)
  • Author(s): 三木崇範
  • Organizer: 第52回日本未熟児新生児学会
  • Place of Presentation: サンポート高松
  • Year and Date: 20071124-20071126
• [Presentation] Takeuchi Y Short-term ethanol exposure caused changes in neurotrophins and its receptors expression in mice cerebellum. (2007)
  • Author(s): ang ZY, Miki T, Yokoyama T, Matsumoto Y, Warita K, Sumitani K, Lu F
  • Organizer: 第62回日本解剖学会地方会
  • Place of Presentation: 川崎医科大学
  • Year and Date: 20071027-20071028
• [Presentation] Fos-immunoreactive neurons in the lateral central nucleus of amygdala activated by hypotension with special reference to the parabrachial nucleus in the rat (2007)
  • Author(s): akeuchi Y, Xie Q, Matsumoto Y, Warita K, Miki T.
  • Organizer: Italian Society of Neuroscience, Palazzo della Ragione e Palazzo della Gran Guardia
  • Place of Presentation: Verona, Italy
  • Year and Date: 20070927-20090930
• [Presentation] Funa K A nobel enhancer of the PDGF beta-receptor gene activated uring the differentiation of neuroblastoma through GATA factor (2007)
  • Author(s): Matsumoto Y, Kaneko M, Yang W, Watt F, Warita K, Miki T, Takeuchi Y
  • Organizer: Italian Society of Neuroscience, Palazzo della Ragione e Palazzo della Gran Guardia
  • Place of Presentation: Verona, Italy
  • Year and Date: 20070927-20070930
• [Presentation] 中枢神経発達時期におけるイソフルラン曝露の影響:嗅球における神経栄養因子の変化 (2007)
  • Author(s): 三木崇範、横山俊史、植木正明、汪治宇、割田克彦、松本由樹、前川信博、竹内義喜
  • Organizer: 第47回日本先天異常学会学術集会
  • Place of Presentation: 名古屋国際会議場
  • Year and Date: 20070707-20070709
• [Presentation] Neuroprotective Effects of Rare Sugar against Ethanol Toxicity; An Electron Microscopic Study in Mice Hippocampus. (2006)
  • Author(s): Takeuchi Y, Yokoyama T, Miki T, Wang ZY, Yang W, Ueno M.
  • Organizer: Rare Sugar Congress 2006 in Kagawa, The 3rd Symposium of International Society of Rare Sugars
  • Place of Presentation: かがわ国際会議場
  • Year and Date: 20061121-20061124
• [Presentation] Activity of a novel PDGF b-receptor enhancer during the cel1 cycle and upon differentiation of neuroblastoma (2006)
  • Author(s): Yang W, Kaneko M, Matsumoto Y, Wang ZY, Yokoyama T, Miki T, Watt F, Funa K, Takeuchi Y.
  • Organizer: differentiation of neuroblastoma.第61回日本解剖学会地方会
  • Place of Presentation: 広島大学
  • Year and Date: 20061111-20061112
• [Presentation] 母子間相互作用の発達神経科学解析;母性剥奪による脳発達への影響 (2006)
  • Author(s): 三木崇範、横山俊史、日下隆、汪治宇、松本由樹、楊衛文、伊藤進、竹内義喜
  • Organizer: 第6回日本赤ちゃん学会
  • Place of Presentation: 福井商工会議所
  • Year and Date: 20061111-20061112
• [Presentation] 組織定量解析法(ステレオロジー)を用いた中枢神経系の機能解析 (2006)
  • Author(s): 三木崇範
  • Organizer: 第46回日本先天異常学会学術集会
  • Place of Presentation: 山形テルサ
  • Year and Date: 20060629-20060630
• [Presentation] Activity of a novel PDGF beta-receptor enhancer during the cell cycle and upon differentiation of neuroblastoma (2006)
  • Author(s): Kaneko M, Yang W, Matsumoto Y, Watt F, Funa K.
  • Organizer: The 2nd IFOM-IEO, IFOM-IEO Campus
  • Place of Presentation: Milano, Italy
  • Year and Date: 20060505-20060508
• [Presentation] 長期アルコール摂取によるラット海馬のミエリン構成たんぱく質mRNAの発現量変化 (2006)
  • Author(s): 三木崇範、横山俊史、李京烈、岡本華代、山岡一平、襖田和敏、今川智敬、竹内義喜
  • Organizer: 第111回日本解剖学会
  • Place of Presentation: 北里大学
  • Year and Date: 20060329-20060331
• [Book] Effects of electric or magnetic brain stimulation on cortical and subcortical neuron functions in rats. (2007)
  • Author(s): Touge T, Gonzalez D, Miki T, Hiramine C, Takeuchi H.
  • Total Pages: 515-516
  • Publisher: In Complex Medical Engineering, Springer