2007 Fiscal Year Final Research Report Summary
Analysis of NF-κ B/Iκ B signaling pathway on Iκ Bζ deficient mice
| Project/Area Number |
18591256
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Dermatology
|
|---|
| Research Institution | Kyoto Prefectural University of Medicine |
|---|
Principal Investigator |
UEDA Eiichiro Kyoto Prefectural University of Medicine, Department of Dermatology, Assistant Professor (40360036)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
UETA Mayumi Kyoto Prefectural University of Medicine, Department of Dermatology, Resident (60398386)
|
|---|
| Project Period (FY) |
2006 – 2007
|
| Keywords | IκBζ / innate immunity / dermatitis |
|---|
| Research Abstract |
Purpose: IκBζ-/- mice were reported to be affected by allergic dermatitis. To analyze the pathophysiological role of IκBζ; and to address the functional relevance of Th2-mediated immune responses in the development of ocular surface inflammation and dermatitis by IκBζ-/- mice.
Methods: We established Balb/c background IκBζ-/- mice without individual differences, created IκBζ/Stat6 double-knock-out (WKO) mice unable to produce Th2 cytokine, and performed microscopic-, histological-, and immunochemical studies. In IκBζ-/- mice we examined the serum IgE levels by ELISA and used quantitative PCR to study the gene expression of IFN-γ, IL4, IL10, TNFα, IL6, IL17α, and CCL11 in eyelid tissue.
Results: IκBζ,-/- mice exhibited a severe inflammatory phenotype on the ocular surface and perioral skin. The inflammatory infiltrates in the perioral skin consisted primarily of CD4- and CD8-positive cells; in the conjunctiva we mainly detected CD4- and CD45R/B220-positive cells. In eyelid and perioral skin tissue the expression of IL-17a and of Th1 and Th2 cytokines, but not of CCL11, was augmented. IκBζ-/- and IκBζ-I+ mice did not differ significantly in their serum total IgE levels before- and 0-4- and 5-9 weeks after disease onset. IκBζ/Stat6 WKO mice elicited the same or a little more severe inflammation than that of IκBζ-/- mice.
Conclusion: IgE and Stat6 are not responsible for the immune-pathological response leading to the development of ocular surface and perioral skin inflammation in IκBζ-/- mice. IκBζ-/- mice might be a suitable model for Stevens-Johnson syndrome, but not atopic dermatitis.
|
-
-
[Journal Article] Human conjunctival epithelial cells express functional Toll-like receptor 52008
Author(s)
Kojima, K., Ueta, M., Hamuro, J., Hozono, Y., Kawasaki, S., Yokoi, N., Kinoshita S
-
Journal Title
Br J Ophthalmol 92
Pages: 411-6
Description
「研究成果報告書概要(欧文)」より
-
[Journal Article] Stat6-independent tissue inflammation occurs selectively on the ocular surface and perioral skin of I{kappa)B{zeta)-/-mice2008
Author(s)
Ueta, M., Hamuro, J., Ueda, E., Katoh, N., Yamamoto, M., Takeda, K., Akira, S., Kinoshita, S
-
Journal Title
Invest Ophthalmol Vis Sci (in press)
Description
「研究成果報告書概要(欧文)」より
-
-
-
-
-
[Journal Article] Polyclonality of Staphylococcus epidermidis residing on the healthy ocular surface2007
Author(s)
Ueta, M., Iida, T, Sakamoto, M., Sotozono, C, Takahashi, J, Kojima, K, Okada, K, Chen, X, Kinoshita, S, Honda, T
-
Journal Title
J Med Microbiol 56
Pages: 77-82
Description
「研究成果報告書概要(欧文)」より
-
[Journal Article] The Role of Platelets in Leukocyte Recruitment in Chronic Contact Hypersensitivity Induced by Repeated Elicitation2007
Author(s)
Tamagawa, R., Katoh, N., Ueda, E., Takenaka, H., Kita, M., Kishimoto, S
-
Journal Title
Am J Pathol 170
Pages: 2019-2029
Description
「研究成果報告書概要(欧文)」より
-
-
-
[Journal Article] Association of IL4R polymorphisms with Stevens-Johnson syndrome2007
Author(s)
Ueta, M., Sotozono, C., Inatomi, T., Kojima, K., Hamuro, J., Kinoshita, S
-
Journal Title
J Allergy Clin Immunol 120
Pages: 1457-9
Description
「研究成果報告書概要(欧文)」より
-
-
[Journal Article] Cytomegalovirus in cutaneous ulcers in a patients with adult T-cell Lymphoma2006
Author(s)
Saito, Y., Takenaka, H., Chin, T., Ueda, E., Katoh, N., Shimazaki, C., Inaba, R., Kishimoto, S
-
Journal Title
Acta Derm Venereol 86
Pages: 181-182
Description
「研究成果報告書概要(欧文)」より
-
[Journal Article] Pedunculated Lipofibroma (Hoffmann-Zurhelle) on the Palm2006
Author(s)
Hanada, K., Takenaka, H., Asai, J., Ueda, E., Katoh, N., Kishimoto, S
-
Journal Title
Acta DermVenereol 86
Pages: 559-560
Description
「研究成果報告書概要(欧文)」より
-
[Journal Article] Successful treatment of diabetic gangrene with topical application of a mixture of peripheral blood mononuclear cells and basic fibroblast growth factor2006
Author(s)
Asai, J., Takenaka, H., Ichihashi, K., Ueda, E., Katoh, N., Kishimoto, S
-
Journal Title
J Dermatol 33
Pages: 349-352
Description
「研究成果報告書概要(欧文)」より
-
-
[Journal Article] Human corneal epithelial cells respond to ocular-pathogenic, but not to nonpathogenic-flagellin2006
Author(s)
Hozono, Y., Ueta, M., Hamuro, J., Kojima, K., Kawasaki, S., Yamazaki, K., Kinoshita, S
-
Journal Title
Biochem Biophys Res Commun 347
Pages: 238-247
Description
「研究成果報告書概要(欧文)」より
