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2007 Fiscal Year Final Research Report Summary

The role of BMP and Kit in melanocyte and melanoma cell differentiation

Research Project

Project/Area Number 18591261
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Dermatology
Research InstitutionSt.Marianna University School of Medicine

Principal Investigator

KAWAKAMI Tamihiro  St.Marianna University School of Medicine, Department of Dermatology, Associate professor (20297659)

Project Period (FY) 2006 – 2007
KeywordsNeural crest cells / Melanocyte / BMP / Kit / Primary culture of NCC / noggin / Melanoblast / Melanoma
Research Abstract

Genes encoding Kit and the Kit ligand (RI) play essential roles in the differentiation of melanoblasts. We previously established three immortal but distinct cell populations of mouse neural crest (NC) cells. NCCmelb4M5 cells do not express Kit and grow independently of KL; they have the potential to differentiate into NCCmelb4 cells, which are Kit-positive melanocyte precursors. NCCmelan5 cells show the characteristics of differentiated melanocytes. All three cell lines demonstrated BMP-receptor expression. BMP-4 upregulated Kit protein and mRNA expression in most immature NCCmelb4M5 cells. Noggin, a BMP-4 antagonist, dramatically decreased the Kit expression induced by BMP-4. Western blot analysis revealed that extrinsic BMP-4 leads to the phosphorylation of Smads in NCCmelb4M5 cells. Using transfected Kit-promoter reporter we showed BMP-4 could activate Kit promoter in transfected NCCmelb4M5 cells. We conclude that BMP-4 is active and is involved in the regulation of Kit expression on most immature melanocyte precursors. We further investigated the influence of BMP-4 in vitro using primary NC cells cultured from wild-type mice. Addition of BMP-4 to the medium increased the number of Kit-positive cells compared to diluent-treated controls. We have identified BMP-4 as an important factor for prenatal Kit-negative melanoblasts just prior to entering the Kit-dependent cycle of melanogenesis.

  • Research Products

    (17 results)

All 2008 2007

All Journal Article (14 results) (of which Peer Reviewed: 7 results) Presentation (2 results) Book (1 results)

  • [Journal Article] BMP-4 upregulates Kit expression in mouse melanoblasts prior to the Kit-dependent cycle of melanogenesis2008

    • Author(s)
      Kawakami T, et. al.
    • Journal Title

      Journal of Investigative Dermatology 128(5)

      Pages: 1220-1226

    • Description
      「研究成果報告書概要(和文)」より
    • Peer Reviewed
  • [Journal Article] Proteomic analysis of immature murine melanocytes at different stages of maturation:A crucial role for calreticulin2008

    • Author(s)
      Kawase A, et. al.
    • Journal Title

      Journal of Dermatological Science 49(1)

      Pages: 43-52

    • Description
      「研究成果報告書概要(和文)」より
    • Peer Reviewed
  • [Journal Article] High titer of serum antiphospholipid antibodies levels in adult Henoch-Schonlein purpura and cutaneous leukocytoclastic angiitis2008

    • Author(s)
      Kawakami T, et. al.
    • Journal Title

      Arthritis&Rheumtism 59(4)

      Pages: 561-567

    • Description
      「研究成果報告書概要(和文)」より
    • Peer Reviewed
  • [Journal Article] BMP-4 upregulates Kit expression in mouse melanoblasts prior to the Kit-dependent cycle of melanogenesis.2008

    • Author(s)
      Kawakami T
    • Journal Title

      J Invest Dermatol. 128(5)

      Pages: 1220-1226

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Proteomic analysis of immature murine melanocytes at different stages of maturation : A crucial role for calreticulin.2008

    • Author(s)
      Kawase A
    • Journal Title

      J Derrnatol Sci. 49(1)

      Pages: 43-52

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] High titer of serum antiphospholipid antibodies levels in adult Henoch-Schonlein purpura and cutaneous leukocytoclastic angiitis.2008

    • Author(s)
      Kawakami T
    • Journal Title

      Arthritis Rheum 59(4)

      Pages: 561-567

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Expression of matrilysin(matrix metalloproteinase-7)in primary cutaneous and metastatic melanoma2007

    • Author(s)
      Kawasaki K, et. al.
    • Journal Title

      British Journal of Dermatology 156(4)

      Pages: 613-619

    • Description
      「研究成果報告書概要(和文)」より
    • Peer Reviewed
  • [Journal Article] Sphingosine 1-phosphate accelerates wound healing in diabetic mice2007

    • Author(s)
      Kawanabe T, et. al.
    • Journal Title

      Journal of Dermatological Science 48(1)

      Pages: 53-60

    • Description
      「研究成果報告書概要(和文)」より
    • Peer Reviewed
  • [Journal Article] High titer of anti-phosphatidylserine-prothrombin complex antibodies in patients with cutaneous polyarteritis nodosa2007

    • Author(s)
      Kawakami T, et. al.
    • Journal Title

      Arthritis&Rheumtism 57(8)

      Pages: 1507-1513

    • Description
      「研究成果報告書概要(和文)」より
    • Peer Reviewed
  • [Journal Article] TGF-beta overexpression in cutaneous extramedullary hematopoiesis of a patient with myelodysplastic syndrome associated with myelofibrosis2007

    • Author(s)
      Kawakami T, et. al.
    • Journal Title

      Journal of The American Academy of Dermatology 58(4)

      Pages: 703-706

    • Description
      「研究成果報告書概要(和文)」より
    • Peer Reviewed
  • [Journal Article] Expression of matrilysin(matrix metalloproteinase-7) in primary cutaneous and metastatic melanoma.2007

    • Author(s)
      Kawasaki K
    • Journal Title

      Br J Dermatol. 156(4)

      Pages: 613-619

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Sphingosine 1-phosphate accelerates wound healing in diabetic mice.2007

    • Author(s)
      Kawanabe T
    • Journal Title

      J Dermatol Sci. 48(1)

      Pages: 53-60

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] High titer of anti-phosphatidylserine-prothrombin complex antibodies in patients with cutaneous polyarteritis nodosa.2007

    • Author(s)
      Kawakami T
    • Journal Title

      Arthritis Rheum. 57(8)

      Pages: 1507-1513

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Transforming growth factor-beta overexpression in cutaneous extramedullary hematopoiesis of a patient with myelodysplastic syndrome associated with myelofibrosis.2007

    • Author(s)
      Kawakami T
    • Journal Title

      J Am Acad Dermatol. 58(4)

      Pages: 703-706

    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] BMP-4 regulates melanocyte proliferation and differentiation in mouse neural crest cells just before they enter the Kit-dependent cycle of melanogenesis2007

    • Author(s)
      Kawakami T, et. al.
    • Organizer
      14th European Society for Pigment Cell Research
    • Place of Presentation
      Bari,Italy
    • Year and Date
      20071014-17
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] BMP-4 regulates melanocyte proliferation and differentiation in mouse neural crest cells just before they enter the Kit-dependent cycle of melanogenesis2007

    • Author(s)
      Kawakami T, et. al.
    • Organizer
      14th European Society for Pigment Cell Research
    • Place of Presentation
      Bari, Italy
    • Year and Date
      20071014-17
    • Description
      「研究成果報告書概要(欧文)」より
  • [Book] 皮膚科診療プラクティス20巻 Enviromental Dermatology外的刺激によるメラニン合成の変調2007

    • Author(s)
      川上 民裕
    • Total Pages
      24-30
    • Publisher
      文光堂
    • Description
      「研究成果報告書概要(和文)」より

URL: 

Published: 2010-02-04  

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