micro RNAs and maintenance of keratinocyte functions

2007 Fiscal Year Final Research Report Summary

• Project/Area Number: 18591266
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Dermatology
• Research Institution: Hyogo College of Medicine
• Principal Investigator: YAMANISHI Kiyofumi Hyogo College of Medicine, Faculty of Medicine, Professor (10182586)
• Project Period (FY): 2006 – 2007
• Keywords: skin / epidermis / keratinocytes / gene regulation / RNA / microarray / マイクロアレイ

Research Abstract

MicroRNAs (miRNA) are functional RNAs that regulate gene expression in groups. Recently, miRNAs have been supposed to be involved in pathophysiology of a variety of diseases. In the present study, we have focused on the role of miRNAs in keratinocyte growth and differentiation and on the regulation of downstream genes. When cultured human keratinocytes were incubated in a high Ca2+ condition, a screening using mircroarray revealed that the expression of several miRNAs including hsa-let7b and hsa-miR-29a was altered. The changes were confirmed by real-time quantitative PCR. The transfection of hsa-miR-29a into keratinocytes significantly suppressed the expression of several genes including transglutaminase 1 gene (TGM1). Lacking TGM in mice leads to the changes in the expression of the genes for cholesterol metabolism and a serine protease in the epidermis, and therefore, has-miR-29a regulates those genes upstream to maintain keratinocyte cellular functions.

Research Products

• [Journal Article] The effect of 14-membered ring macrolides on the production of interleukin-8 mediated by protease-activated receptor 2 in human keratinocytes (2008)
  • Author(s): Tatsuya Tsuda
  • Journal Title: Antimicrobial Agents and Chemotherapy 52 Pages: 1538-1541
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] The effect of 14-membered ring macrolides on the production of interleukin-8 mediated by protease-activated receptor 2 in human keratinocytes. (2008)
  • Author(s): Tsuda T, Ishikawa C, Konishi H, Hayashi Y, Nakagawa N, Matsuki M, Mizutani H, Yamanishi K.
  • Journal Title: Antimictob Agents Chemother 52 Pages: 1538-1541
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Contribution of interleukin-18 to atopic dermatitis-like skin inflammation induced by Staphylococcus aureus product in mice (2006)
  • Author(s): Makoto Terada
  • Journal Title: Proceedings of the National Academy of Science, USA 103 Pages: 8816-8821
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Human mast cell chymase cleaves pro-IL-18 and generates a novel and biologically active IL-18 fragment (2006)
  • Author(s): Yohichi Omoto
  • Journal Title: Journal of Immunology 177 Pages: 8315-8319
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Human mast cell chymase cleaves pro-IL-18 and generates a novel and biologically active IL-18 fragment. (2006)
  • Author(s): Omoto Y, Tokime K, Yamanaka K, Habe K, Morioka T, Kurokawa I, Tsutsui H, Yamanishi K, Nakanishi, K, Mizutani H.
  • Journal Title: J Immunol 177 Pages: 8315-8319
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Contribution of interleukin-18 to atopic dermatitis-like skin inflammation induced by Staphylococcus aureus product in mice. (2006)
  • Author(s): Terada M, Tsutsui H, Yasutomo I, Yasuda K, Mizutani H, Yamanishi K, Kubo M, Matsui K, Sano H, Nakanishi K.
  • Journal Title: Proc Natl Acad Sci USA 103 Pages: 8816-8821
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Functional genomic analysis of skin barrier defects with deranged molecular organization of stratum corneum intercellular lipids in mice lacking transglutaminase 1. (2007)
  • Author(s): Nakagawa N, Tsuda T, Ohta N, Matsuki M, Yagi N, Hatta I, Tsujimoto G, Yamanishi K.
  • Organizer: 13th European congress on Biotechnology
  • Place of Presentation: Barcelona, Spain
  • Year and Date: 20070916-19
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Functional genomic analysis of skin barrier defects with deranged molecular organization of stratum corneum intercellular lipids in mice lacking transglutaminase 1 (2007)
  • Author(s): Noboru Nakagawa
  • Organizer: 13th European Congress on Biotechnology
  • Place of Presentation: バルセロナ,スペイン
  • Year and Date: 2007-09-16
  • Description: 「研究成果報告書概要(和文)」より
• [Book] 今日の治療指針 (2007)
  • Author(s): 山西 清文
  • Total Pages: 849
  • Publisher: 医学書院
  • Description: 「研究成果報告書概要(和文)」より